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Differences in estimates of cisplatin-induced cell kill in vitro between colorimetric and cell count/colony assays

Henriksson, Eva LU ; Kjellén, Elisabeth LU ; Wahlberg, Peter LU ; Wennerberg, Johan LU orcid and Kjellström, Johan LU (2006) In In Vitro Cellular & Developmental Biology - Animal 42(10). p.320-323
Abstract
The aim of this study was to evaluate some bioassays that are different in principle: cell counting, colony forming assay, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT), sulforhodamine B (SRB), crystal violet, and alamarBlue, with respect to their ability to measure cisplatin-induced cell death of in vitro-cultivated squamous cell carcinoma of the head and neck (SCCHN). Cisplatin was applied in concentrations of 1.0, 5.0, 10.0, 50.0, and 100 mu M. The cells were incubated for 1 h, and the cell survival was measured 5 d after treatment. We found the colorimetric assays and cell counting to be comparable. The colony forming assay indicated a higher degree of cell kill compared with the other techniques. Measurement of... (More)
The aim of this study was to evaluate some bioassays that are different in principle: cell counting, colony forming assay, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT), sulforhodamine B (SRB), crystal violet, and alamarBlue, with respect to their ability to measure cisplatin-induced cell death of in vitro-cultivated squamous cell carcinoma of the head and neck (SCCHN). Cisplatin was applied in concentrations of 1.0, 5.0, 10.0, 50.0, and 100 mu M. The cells were incubated for 1 h, and the cell survival was measured 5 d after treatment. We found the colorimetric assays and cell counting to be comparable. The colony forming assay indicated a higher degree of cell kill compared with the other techniques. Measurement of cell survival after treatment with cisplatin can be done by use of any of the above tested assays. However, the majority of SCCHN cell lines available do not form colonies easily, or at all. Therefore, comparing the chemosensitivity between such cell lines is limited to alternative assays. In this respect, any of the tested colorimetric assays can be used. However, they seem to underestimate cell kill. Cell counting is also an alternative. This technique, however, is time consuming and operator dependent, as in the ease of manual counting, or relatively expensive when counting is performed electronically, compared with the colorimetric assays. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
crystal violet, alamarBlue, in vitro, MTT, SRB
in
In Vitro Cellular & Developmental Biology - Animal
volume
42
issue
10
pages
320 - 323
publisher
Springer
external identifiers
  • wos:000243666400008
  • scopus:33846860220
ISSN
1071-2690
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Otorhinolaryngology (Lund) (013044000), Oncology, MV (013035000), Reconstructive Surgery (013240300)
id
4dd0a66a-b4aa-42a1-bbbb-7ae8c630ca7a (old id 676631)
date added to LUP
2016-04-01 16:18:47
date last changed
2021-10-06 03:41:29
@article{4dd0a66a-b4aa-42a1-bbbb-7ae8c630ca7a,
  abstract     = {The aim of this study was to evaluate some bioassays that are different in principle: cell counting, colony forming assay, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT), sulforhodamine B (SRB), crystal violet, and alamarBlue, with respect to their ability to measure cisplatin-induced cell death of in vitro-cultivated squamous cell carcinoma of the head and neck (SCCHN). Cisplatin was applied in concentrations of 1.0, 5.0, 10.0, 50.0, and 100 mu M. The cells were incubated for 1 h, and the cell survival was measured 5 d after treatment. We found the colorimetric assays and cell counting to be comparable. The colony forming assay indicated a higher degree of cell kill compared with the other techniques. Measurement of cell survival after treatment with cisplatin can be done by use of any of the above tested assays. However, the majority of SCCHN cell lines available do not form colonies easily, or at all. Therefore, comparing the chemosensitivity between such cell lines is limited to alternative assays. In this respect, any of the tested colorimetric assays can be used. However, they seem to underestimate cell kill. Cell counting is also an alternative. This technique, however, is time consuming and operator dependent, as in the ease of manual counting, or relatively expensive when counting is performed electronically, compared with the colorimetric assays.},
  author       = {Henriksson, Eva and Kjellén, Elisabeth and Wahlberg, Peter and Wennerberg, Johan and Kjellström, Johan},
  issn         = {1071-2690},
  language     = {eng},
  number       = {10},
  pages        = {320--323},
  publisher    = {Springer},
  series       = {In Vitro Cellular & Developmental Biology - Animal},
  title        = {Differences in estimates of cisplatin-induced cell kill in vitro between colorimetric and cell count/colony assays},
  volume       = {42},
  year         = {2006},
}