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An experimental and theoretical study of the morphine binding capacity and kinetics of an engineered opioid receptor

Kriz, Kirstin LU ; Debeljak, Natasa; Wärnmark, Ioana LU and Kriz, Dario (2007) In Biosensors & Bioelectronics 22(6). p.1168-1171
Abstract
Electrochemical real-time monitoring of ligand binding to an engineered opioid receptor specific for morphine is reported. In the particular systems studied, 90% of the binding was found to be completed after only 85-120 s. Thus, the binding kinetics has proven to be more rapid than previously believed. The observed association rate constant for the morphine binding reaction was calculated to be 215 M-1 s(-1). A theoretical analysis of the experimental binding data suggested that the binding sites of the engineered opioid receptor could best be described by a model having two populations of binding sites: K-D = 40 mu M (13 mu mol/g) and K-D = 205 mu M (29 mu mol/g). Furthermore, a theoretical model was developed in order to explain the... (More)
Electrochemical real-time monitoring of ligand binding to an engineered opioid receptor specific for morphine is reported. In the particular systems studied, 90% of the binding was found to be completed after only 85-120 s. Thus, the binding kinetics has proven to be more rapid than previously believed. The observed association rate constant for the morphine binding reaction was calculated to be 215 M-1 s(-1). A theoretical analysis of the experimental binding data suggested that the binding sites of the engineered opioid receptor could best be described by a model having two populations of binding sites: K-D = 40 mu M (13 mu mol/g) and K-D = 205 mu M (29 mu mol/g). Furthermore, a theoretical model was developed in order to explain the observed binding of the engineered opioid receptor. This model suggested that the binding sites on the polymer surface are up to 5.1 A deep and they allow 100% of the ligand (morphine) to anchor itself into the site. The predicted theoretical maximum binding capacity for the reported receptor is calculated to be approximately 2 mmol/g polymer (based on an increase of cavity density). (c) 2006 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
binding kinetics, MIP, morphine
in
Biosensors & Bioelectronics
volume
22
issue
6
pages
1168 - 1171
publisher
Elsevier
external identifiers
  • wos:000243541800058
  • scopus:33845450783
ISSN
1873-4235
DOI
10.1016/j.bios.2006.09.024
language
English
LU publication?
yes
id
81be28da-c203-48c2-ba26-ad5e48fc6795 (old id 677556)
date added to LUP
2007-12-12 09:50:08
date last changed
2017-01-01 07:04:03
@article{81be28da-c203-48c2-ba26-ad5e48fc6795,
  abstract     = {Electrochemical real-time monitoring of ligand binding to an engineered opioid receptor specific for morphine is reported. In the particular systems studied, 90% of the binding was found to be completed after only 85-120 s. Thus, the binding kinetics has proven to be more rapid than previously believed. The observed association rate constant for the morphine binding reaction was calculated to be 215 M-1 s(-1). A theoretical analysis of the experimental binding data suggested that the binding sites of the engineered opioid receptor could best be described by a model having two populations of binding sites: K-D = 40 mu M (13 mu mol/g) and K-D = 205 mu M (29 mu mol/g). Furthermore, a theoretical model was developed in order to explain the observed binding of the engineered opioid receptor. This model suggested that the binding sites on the polymer surface are up to 5.1 A deep and they allow 100% of the ligand (morphine) to anchor itself into the site. The predicted theoretical maximum binding capacity for the reported receptor is calculated to be approximately 2 mmol/g polymer (based on an increase of cavity density). (c) 2006 Elsevier B.V. All rights reserved.},
  author       = {Kriz, Kirstin and Debeljak, Natasa and Wärnmark, Ioana and Kriz, Dario},
  issn         = {1873-4235},
  keyword      = {binding kinetics,MIP,morphine},
  language     = {eng},
  number       = {6},
  pages        = {1168--1171},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {An experimental and theoretical study of the morphine binding capacity and kinetics of an engineered opioid receptor},
  url          = {http://dx.doi.org/10.1016/j.bios.2006.09.024},
  volume       = {22},
  year         = {2007},
}