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Effect of matrix elasticity on affinity binding and release of bioparticles. Elution of bound cells by temperature-induced shrinkage of the smart macroporous hydrogel

Galaev, Igor LU ; Dainiak, Maria LU ; Plieva, Fatima LU and Mattiasson, Bo LU (2007) In Langmuir 23(1). p.35-40
Abstract
The first step of bacterial or viral invasion is affinity and presumably multisite binding of bioparticles to an elastic matrix like a living tissue. We have demonstrated that model bioparticles such as inclusion bodies (spheres of about 1 mu m in size) Escherichia coli cells (rods 1 x 3 mu m), yeast cells (8 mu m spheres), and synthetic microgel particles (0.4 mu m spheres) are binding via different affinity interactions (IgG antibody-protein A, sugar-lectin, and metal ion-chelate) to a macroporous hydrogel (MH) matrix bearing appropriate ligands. The elastic deformation of the MH results in the detachment of affinity bound bioparticles. The particle detachment on elastic deformation is believed to be due to multipoint attachment of the... (More)
The first step of bacterial or viral invasion is affinity and presumably multisite binding of bioparticles to an elastic matrix like a living tissue. We have demonstrated that model bioparticles such as inclusion bodies (spheres of about 1 mu m in size) Escherichia coli cells (rods 1 x 3 mu m), yeast cells (8 mu m spheres), and synthetic microgel particles (0.4 mu m spheres) are binding via different affinity interactions (IgG antibody-protein A, sugar-lectin, and metal ion-chelate) to a macroporous hydrogel (MH) matrix bearing appropriate ligands. The elastic deformation of the MH results in the detachment of affinity bound bioparticles. The particle detachment on elastic deformation is believed to be due to multipoint attachment of the particles to affinity matrix and the disturbance of the distance between affinity ligands when the matrix is deformed. No release of affinity bound protein occurred on elastic deformation. The efficiency of the particle release by the elastic deformation depends on the density of the ligands at the particle surface as well as on the elasticity of the matrix for relatively large particles. The release of the particles occurred irrespectively of whether the deformation was caused by external forces (mechanical deformation) or internal forces (the shrinkage of thermosensitive macroporous poly-N-isopropylacrylamide hydrogel on increase in temperature). (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
23
issue
1
pages
35 - 40
publisher
The American Chemical Society
external identifiers
  • wos:000243086600008
  • scopus:33846384305
ISSN
0743-7463
DOI
10.1021/la061462e
language
English
LU publication?
yes
id
d4fe33cf-020e-4e96-b5f9-cb89dddcf46a (old id 679995)
date added to LUP
2007-12-07 11:51:06
date last changed
2017-02-19 03:23:52
@article{d4fe33cf-020e-4e96-b5f9-cb89dddcf46a,
  abstract     = {The first step of bacterial or viral invasion is affinity and presumably multisite binding of bioparticles to an elastic matrix like a living tissue. We have demonstrated that model bioparticles such as inclusion bodies (spheres of about 1 mu m in size) Escherichia coli cells (rods 1 x 3 mu m), yeast cells (8 mu m spheres), and synthetic microgel particles (0.4 mu m spheres) are binding via different affinity interactions (IgG antibody-protein A, sugar-lectin, and metal ion-chelate) to a macroporous hydrogel (MH) matrix bearing appropriate ligands. The elastic deformation of the MH results in the detachment of affinity bound bioparticles. The particle detachment on elastic deformation is believed to be due to multipoint attachment of the particles to affinity matrix and the disturbance of the distance between affinity ligands when the matrix is deformed. No release of affinity bound protein occurred on elastic deformation. The efficiency of the particle release by the elastic deformation depends on the density of the ligands at the particle surface as well as on the elasticity of the matrix for relatively large particles. The release of the particles occurred irrespectively of whether the deformation was caused by external forces (mechanical deformation) or internal forces (the shrinkage of thermosensitive macroporous poly-N-isopropylacrylamide hydrogel on increase in temperature).},
  author       = {Galaev, Igor and Dainiak, Maria and Plieva, Fatima and Mattiasson, Bo},
  issn         = {0743-7463},
  language     = {eng},
  number       = {1},
  pages        = {35--40},
  publisher    = {The American Chemical Society},
  series       = {Langmuir},
  title        = {Effect of matrix elasticity on affinity binding and release of bioparticles. Elution of bound cells by temperature-induced shrinkage of the smart macroporous hydrogel},
  url          = {http://dx.doi.org/10.1021/la061462e},
  volume       = {23},
  year         = {2007},
}