Advanced

Improved positioning and detectability of microparticles in droplet microfluidics using two-dimensional acoustophoresis

Ohlin, M.; Fornell, A. LU ; Bruus, H. and Tenje, M. LU (2017) Micromechanics and Microsystems Europe Conference, 2016 In Journal of Micromechanics and Microengineering 27(8).
Abstract

We have fabricated a silicon-glass two-phase droplet microfluidic system capable of generating sub 100 μm-sized, ø = (74 ± 2) μm, spherical droplets at rates of up to hundreds of hertz. By implementing a two-dimensional (2D) acoustophoresis particle-positioning method, we show a fourfold improvement in both vertical and lateral particle positioning inside the droplets compared to unactuated operation. The efficiency of the system has been optimized by incorporating aluminum matching layers in the transducer design permitting biocompatible operational temperatures (<37 °C). Furthermore, by using acoustic actuation, (99.8 ± 0.4)% of all encapsulated microparticles can be detected compared to only (79.0 ± 5.1)% for unactuated operation.... (More)

We have fabricated a silicon-glass two-phase droplet microfluidic system capable of generating sub 100 μm-sized, ø = (74 ± 2) μm, spherical droplets at rates of up to hundreds of hertz. By implementing a two-dimensional (2D) acoustophoresis particle-positioning method, we show a fourfold improvement in both vertical and lateral particle positioning inside the droplets compared to unactuated operation. The efficiency of the system has been optimized by incorporating aluminum matching layers in the transducer design permitting biocompatible operational temperatures (<37 °C). Furthermore, by using acoustic actuation, (99.8 ± 0.4)% of all encapsulated microparticles can be detected compared to only (79.0 ± 5.1)% for unactuated operation. In our experiments we observed a strong ordering of the microparticles in distinct patterns within the droplet when using 2D acoustophoresis; to explain the origin of these patterns we simulated numerically the fluid flow inside the droplets and compared with the experimental findings.

(Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
acoustophoresis, droplet microfluidics, microparticle detection, microparticle manipulation, ultrasonic standing wave
in
Journal of Micromechanics and Microengineering
volume
27
issue
8
publisher
IOP Publishing
conference name
Micromechanics and Microsystems Europe Conference, 2016
external identifiers
  • scopus:85026419839
  • wos:000415698300001
ISSN
0960-1317
DOI
10.1088/1361-6439/aa7967
language
English
LU publication?
yes
id
67aaab15-4b63-4fb8-a5a0-234795793011
date added to LUP
2017-08-31 14:04:36
date last changed
2018-02-25 04:19:04
@article{67aaab15-4b63-4fb8-a5a0-234795793011,
  abstract     = {<p>We have fabricated a silicon-glass two-phase droplet microfluidic system capable of generating sub 100 μm-sized, ø = (74 ± 2) μm, spherical droplets at rates of up to hundreds of hertz. By implementing a two-dimensional (2D) acoustophoresis particle-positioning method, we show a fourfold improvement in both vertical and lateral particle positioning inside the droplets compared to unactuated operation. The efficiency of the system has been optimized by incorporating aluminum matching layers in the transducer design permitting biocompatible operational temperatures (&lt;37 °C). Furthermore, by using acoustic actuation, (99.8 ± 0.4)% of all encapsulated microparticles can be detected compared to only (79.0 ± 5.1)% for unactuated operation. In our experiments we observed a strong ordering of the microparticles in distinct patterns within the droplet when using 2D acoustophoresis; to explain the origin of these patterns we simulated numerically the fluid flow inside the droplets and compared with the experimental findings.</p>},
  articleno    = {084002},
  author       = {Ohlin, M. and Fornell, A. and Bruus, H. and Tenje, M.},
  issn         = {0960-1317},
  keyword      = {acoustophoresis,droplet microfluidics,microparticle detection,microparticle manipulation,ultrasonic standing wave},
  language     = {eng},
  month        = {07},
  number       = {8},
  publisher    = {IOP Publishing},
  series       = {Journal of Micromechanics and Microengineering},
  title        = {Improved positioning and detectability of microparticles in droplet microfluidics using two-dimensional acoustophoresis},
  url          = {http://dx.doi.org/10.1088/1361-6439/aa7967},
  volume       = {27},
  year         = {2017},
}