Improved Lectin-Mediated Immobilization of Human Red Blood Cells in Superporous Agarose Beads.
(2003) In Journal of Chromatography. B 784(1). p.203-208- Abstract
- A new type of agarose bead, superporous agarose, was used as a gel support for immobilization of human red blood cells (RBCs) mediated by wheat germ lectin. The number of immobilized cells was similar to that obtained with commercial wheat germ lectin–agarose but the cell stability appeared to be superior. This allowed improved frontal affinity chromatographic analyses of cytochalasin B (CB)-binding to the glucose transporter GLUT1 which established a ratio of one CB-binding site per GLUT1 dimer for both plain RBCs or those treated with different poly amino acids. The measured dissociation constants, 70±14 nM for CB and 12±3 mM for glucose binding to GLUT1, are similar to those reported earlier.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/128607
- author
- Gottschalk, I ; Gustavsson, Per-Erik LU ; Ersson, B and Lundahl, P
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Wheat germ agglutinin, Cytochalasin B, GLUT1
- in
- Journal of Chromatography. B
- volume
- 784
- issue
- 1
- pages
- 203 - 208
- publisher
- Elsevier
- external identifiers
-
- wos:000180412800023
- pmid:12504199
- scopus:0347928849
- ISSN
- 1873-376X
- DOI
- 10.1016/S1570-0232(02)00758-4
- language
- English
- LU publication?
- yes
- id
- 67b75e41-70f9-42e6-9886-370c048ed213 (old id 128607)
- date added to LUP
- 2016-04-01 11:36:55
- date last changed
- 2022-01-26 07:37:35
@article{67b75e41-70f9-42e6-9886-370c048ed213, abstract = {{A new type of agarose bead, superporous agarose, was used as a gel support for immobilization of human red blood cells (RBCs) mediated by wheat germ lectin. The number of immobilized cells was similar to that obtained with commercial wheat germ lectin–agarose but the cell stability appeared to be superior. This allowed improved frontal affinity chromatographic analyses of cytochalasin B (CB)-binding to the glucose transporter GLUT1 which established a ratio of one CB-binding site per GLUT1 dimer for both plain RBCs or those treated with different poly amino acids. The measured dissociation constants, 70±14 nM for CB and 12±3 mM for glucose binding to GLUT1, are similar to those reported earlier.}}, author = {{Gottschalk, I and Gustavsson, Per-Erik and Ersson, B and Lundahl, P}}, issn = {{1873-376X}}, keywords = {{Wheat germ agglutinin; Cytochalasin B; GLUT1}}, language = {{eng}}, number = {{1}}, pages = {{203--208}}, publisher = {{Elsevier}}, series = {{Journal of Chromatography. B}}, title = {{Improved Lectin-Mediated Immobilization of Human Red Blood Cells in Superporous Agarose Beads.}}, url = {{http://dx.doi.org/10.1016/S1570-0232(02)00758-4}}, doi = {{10.1016/S1570-0232(02)00758-4}}, volume = {{784}}, year = {{2003}}, }