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Deuteriation of sugar protons simplify NMR assignments and structure determination of large oligonucleotide by the 1 H-NMR window approach

Chattopadhyaya, J. ; Yamakage, S.-I. ; Maltseva, T.V. ; Nilson, F. P. LU and Földesi, A. (1993) In Nucleic Acids Research 21(22). p.5005-5011
Abstract
The concept of the 1H-NMR window has been developed and examined through a comparative study of NOESY spectra of a self-complementary Dickerson's dodecamer (I) [S'd^C^CGWA^T^T^Ci4GisCi6G) 23'] ) a self-complementary 20-mer (II) [(5d(1 C2G3C4G5C6G7C8G9A1 ˚A11T12T13C14G15C16G17C- 18G19C20G)23'] in which the core part consists of the same Dickerson's dodecamer sequence with the flanking CGCG residues at both 3' and 5'-ends, and the partly-deuteriated (shown by underlined CGCG residues at both 3' and 5'-ends) analogous duplex (III) [5d(1C2G3C4G5C6G7C8G9A10A11T12T13C14G15C16G17C- 18Q19C 2˚G)23'] in which the core 5C to 16G part (i.e.AH-NMR window) consists of the natural Dickerson's dodecamer sequence. A comparison of their NOESY spectra... (More)
The concept of the 1H-NMR window has been developed and examined through a comparative study of NOESY spectra of a self-complementary Dickerson's dodecamer (I) [S'd^C^CGWA^T^T^Ci4GisCi6G) 23'] ) a self-complementary 20-mer (II) [(5d(1 C2G3C4G5C6G7C8G9A1 ˚A11T12T13C14G15C16G17C- 18G19C20G)23'] in which the core part consists of the same Dickerson's dodecamer sequence with the flanking CGCG residues at both 3' and 5'-ends, and the partly-deuteriated (shown by underlined CGCG residues at both 3' and 5'-ends) analogous duplex (III) [5d(1C2G3C4G5C6G7C8G9A10A11T12T13C14G15C16G17C- 18Q19C 2˚G)23'] in which the core 5C to 16G part (i.e.AH-NMR window) consists of the natural Dickerson's dodecamer sequence. A comparison of their NOESY spectra clearly demonstrates that the severe overlap of proton resonances in the larger DNA duplex (II) has been successfully reduced in the partly-deuterated duplex (III) as a result of specific incorporations of the sugar-deuteriated nucleotide residues in the latter [stereospecific ≫97 atom \% 2H enrichment at H2', H2” and H3' sites, - 8 5 atom \% 2H enrichment at H4' and - 2 0 atom \% 2H enrichment at H1' (see refs. 10 and 11) in the 20-mer duplex (III)]. These simplifications of the resonance overlap by the deuteriation approach have enabled unequivocal chemical shift assignments and extraction of the quantitative NOE data in the 1HNMR window part of duplex (III). A comparison of the 12-nucleotide long 1H-NMR window in (III) with that of the 12-mer duplex (I) also shows the scope of studying the changes in conformation and dynamics of the core 12-mer region in (III) which result from the increase of molecular weight due to the DNA chain extension. It is noteworthy that such a study is clearly impossible for the natural 20-mer (II) because of the inherent problem of the overlap of resonances. (Less)
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publishing date
type
Contribution to journal
publication status
published
subject
in
Nucleic Acids Research
volume
21
issue
22
pages
7 pages
publisher
Oxford University Press
external identifiers
  • scopus:0027520470
ISSN
0305-1048
DOI
10.1093/nar/21.22.5005
language
English
LU publication?
no
id
67c3b0ea-2355-43cb-9d0c-8006ef6cc5dc
date added to LUP
2019-09-28 17:09:28
date last changed
2021-01-03 09:13:13
@article{67c3b0ea-2355-43cb-9d0c-8006ef6cc5dc,
  abstract     = {{The concept of the 1H-NMR window has been developed and examined through a comparative study of NOESY spectra of a self-complementary Dickerson's dodecamer (I) [S'd^C^CGWA^T^T^Ci4GisCi6G) 23'] ) a self-complementary 20-mer (II) [(5d(1 C2G3C4G5C6G7C8G9A1 ˚A11T12T13C14G15C16G17C- 18G19C20G)23'] in which the core part consists of the same Dickerson's dodecamer sequence with the flanking CGCG residues at both 3' and 5'-ends, and the partly-deuteriated (shown by underlined CGCG residues at both 3' and 5'-ends) analogous duplex (III) [5d(1C2G3C4G5C6G7C8G9A10A11T12T13C14G15C16G17C- 18Q19C 2˚G)23'] in which the core 5C to 16G part (i.e.AH-NMR window) consists of the natural Dickerson's dodecamer sequence. A comparison of their NOESY spectra clearly demonstrates that the severe overlap of proton resonances in the larger DNA duplex (II) has been successfully reduced in the partly-deuterated duplex (III) as a result of specific incorporations of the sugar-deuteriated nucleotide residues in the latter [stereospecific ≫97 atom \% 2H enrichment at H2', H2” and H3' sites, - 8 5 atom \% 2H enrichment at H4' and - 2 0 atom \% 2H enrichment at H1' (see refs. 10 and 11) in the 20-mer duplex (III)]. These simplifications of the resonance overlap by the deuteriation approach have enabled unequivocal chemical shift assignments and extraction of the quantitative NOE data in the 1HNMR window part of duplex (III). A comparison of the 12-nucleotide long 1H-NMR window in (III) with that of the 12-mer duplex (I) also shows the scope of studying the changes in conformation and dynamics of the core 12-mer region in (III) which result from the increase of molecular weight due to the DNA chain extension. It is noteworthy that such a study is clearly impossible for the natural 20-mer (II) because of the inherent problem of the overlap of resonances.}},
  author       = {{Chattopadhyaya, J. and Yamakage, S.-I. and Maltseva, T.V. and Nilson, F. P. and Földesi, A.}},
  issn         = {{0305-1048}},
  language     = {{eng}},
  month        = {{11}},
  number       = {{22}},
  pages        = {{5005--5011}},
  publisher    = {{Oxford University Press}},
  series       = {{Nucleic Acids Research}},
  title        = {{Deuteriation of sugar protons simplify NMR assignments and structure determination of large oligonucleotide by the 1 H-NMR window approach}},
  url          = {{http://dx.doi.org/10.1093/nar/21.22.5005}},
  doi          = {{10.1093/nar/21.22.5005}},
  volume       = {{21}},
  year         = {{1993}},
}