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Vicia—Micronucleus Test Application for Saline Irrigation Water Risk Assessment

Souguir, Dalila ; Berndtsson, Ronny LU orcid ; Mzahma, Sourour ; Filali, Hanen and Hachicha, Mohamed (2022) In Plants 11(3).
Abstract

In view of climate change, increasing soil salinity is expected worldwide. It is therefore important to improve prediction ability of plant salinity effects. For this purpose, brackish/saline irrigation water from two areas in central and coastal Tunisia was sampled. The water samples were classified as C3 (EC: 2.01–2.24 dS m−1) and C4 (EC: 3.46–7.00 dS m−1), indicating that the water was questionable and not suitable for irrigation, respectively. The water samples were tested for their genotoxic potential and growth effects on Vicia faba seedlings. Results showed a decrease in mitotic index (MI) and, consequently, growth parameters concomitant to the appearance of micronucleus (MCN) and chromosome aberrations when... (More)

In view of climate change, increasing soil salinity is expected worldwide. It is therefore important to improve prediction ability of plant salinity effects. For this purpose, brackish/saline irrigation water from two areas in central and coastal Tunisia was sampled. The water samples were classified as C3 (EC: 2.01–2.24 dS m−1) and C4 (EC: 3.46–7.00 dS m−1), indicating that the water was questionable and not suitable for irrigation, respectively. The water samples were tested for their genotoxic potential and growth effects on Vicia faba seedlings. Results showed a decrease in mitotic index (MI) and, consequently, growth parameters concomitant to the appearance of micronucleus (MCN) and chromosome aberrations when the water salinity increased. Salt ion concentration had striking influence on genome stability and growth parameters. Pearson correlation underlined the negative connection between most ions in the water inappropriate for irrigation (C4) and MI as well as growth parameters. MI was strongly influenced by Mg2+, Na+, Cl−, and to a less degree Ca2+, K+, and SO42−. Growth parameters were moderately to weakly affected by K+ and Ca2+, respectively. Regarding MCN, a very strong positive correlation was found for MCN and K+. Despite its short-term application, the Vicia-MCN Test showed a real ability to predict toxicity induced by salt ions con-firming that is has a relevant role in hazard identification and risk assessment of salinity effects.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cell division, Micronucleus, Plant aberration, Plant growth, Salinity, Vicia faba
in
Plants
volume
11
issue
3
article number
462
publisher
MDPI AG
external identifiers
  • pmid:35161444
  • scopus:85124236623
ISSN
2223-7747
DOI
10.3390/plants11030462
language
English
LU publication?
yes
additional info
Funding Information: Acknowledgments: This work is supported by the Federated Research Project ASHYDRO (PRF2019-D1P6) financed by the Ministry of High Education and Scientific Research, Tunisia. Authors thank all colleagues and the technical staff at the Laboratory of Non-Conventional Water Valorization –INRGREF for water sampling and analysis. The authors acknowledge funding from the European Union Horizon 2020 program FASTER project, grant agreement No [810812] and the MECW (Middle East in the Contemporary World) project at the Centre for Advanced Middle Eastern Studies, Lund University. Funding Information: Funding: The study was funded by the European Union Horizon 2020 program FASTER project, grant agreement No 810812 and the MECW (Middle East in the Contemporary World) project at the Centre for Advanced Middle Eastern Studies, Lund University. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
id
67eea436-c0dc-4d8f-9d63-45c34f51fe1f
date added to LUP
2022-11-11 14:14:32
date last changed
2024-04-04 14:56:52
@article{67eea436-c0dc-4d8f-9d63-45c34f51fe1f,
  abstract     = {{<p>In view of climate change, increasing soil salinity is expected worldwide. It is therefore important to improve prediction ability of plant salinity effects. For this purpose, brackish/saline irrigation water from two areas in central and coastal Tunisia was sampled. The water samples were classified as C3 (EC: 2.01–2.24 dS m<sup>−1</sup>) and C4 (EC: 3.46–7.00 dS m<sup>−1</sup>), indicating that the water was questionable and not suitable for irrigation, respectively. The water samples were tested for their genotoxic potential and growth effects on Vicia faba seedlings. Results showed a decrease in mitotic index (MI) and, consequently, growth parameters concomitant to the appearance of micronucleus (MCN) and chromosome aberrations when the water salinity increased. Salt ion concentration had striking influence on genome stability and growth parameters. Pearson correlation underlined the negative connection between most ions in the water inappropriate for irrigation (C4) and MI as well as growth parameters. MI was strongly influenced by Mg<sup>2+</sup>, Na<sup>+</sup>, Cl<sup>−</sup>, and to a less degree Ca<sup>2+</sup>, K<sup>+</sup>, and SO4<sup>2−</sup>. Growth parameters were moderately to weakly affected by K<sup>+</sup> and Ca<sup>2+</sup>, respectively. Regarding MCN, a very strong positive correlation was found for MCN and K<sup>+</sup>. Despite its short-term application, the Vicia-MCN Test showed a real ability to predict toxicity induced by salt ions con-firming that is has a relevant role in hazard identification and risk assessment of salinity effects.</p>}},
  author       = {{Souguir, Dalila and Berndtsson, Ronny and Mzahma, Sourour and Filali, Hanen and Hachicha, Mohamed}},
  issn         = {{2223-7747}},
  keywords     = {{Cell division; Micronucleus; Plant aberration; Plant growth; Salinity; Vicia faba}},
  language     = {{eng}},
  month        = {{02}},
  number       = {{3}},
  publisher    = {{MDPI AG}},
  series       = {{Plants}},
  title        = {{Vicia—Micronucleus Test Application for Saline Irrigation Water Risk Assessment}},
  url          = {{http://dx.doi.org/10.3390/plants11030462}},
  doi          = {{10.3390/plants11030462}},
  volume       = {{11}},
  year         = {{2022}},
}