Advanced

Zinc export results in adaptive zinc tolerance in the ectomycorrhizal basidiomycete Suillus bovinus

Ruytinx, Joske; Nguyen, Hoai; Van Hees, May; Op De Beeck, Michiel LU ; Vangronsveld, Jaco; Carleer, Robert; Colpaert, Jan V and Adriaensen, Kristin (2013) In Metallomics 5(9). p.33-1225
Abstract

On Zn-polluted soils, populations of the ectomycorrhizal basidiomycete Suillus bovinus exhibit an elevated Zn tolerance when compared to populations on non-polluted sites. To elucidate the mechanism of Zn tolerance, the time-course of Zn uptake was studied in isolates with contrasting Zn tolerance. Unidirectional fluxes and subcellular compartmentation of Zn were investigated through radiotracer flux analyses. Fluorescence imaging was used to support the subcellular Zn compartmentation. After 2 h of exposure to 200 μM Zn, significantly more Zn was accumulated in Zn-sensitive isolates compared to tolerant isolates, despite similar short-term uptake kinetics and similar extracellular Zn sequestration in cell walls. In Zn-sensitive... (More)

On Zn-polluted soils, populations of the ectomycorrhizal basidiomycete Suillus bovinus exhibit an elevated Zn tolerance when compared to populations on non-polluted sites. To elucidate the mechanism of Zn tolerance, the time-course of Zn uptake was studied in isolates with contrasting Zn tolerance. Unidirectional fluxes and subcellular compartmentation of Zn were investigated through radiotracer flux analyses. Fluorescence imaging was used to support the subcellular Zn compartmentation. After 2 h of exposure to 200 μM Zn, significantly more Zn was accumulated in Zn-sensitive isolates compared to tolerant isolates, despite similar short-term uptake kinetics and similar extracellular Zn sequestration in cell walls. In Zn-sensitive isolates twice as much Zn accumulated in the cytoplasm and 12 times more Zn in the vacuole. (65)Zn efflux analyses revealed a considerably faster Zn export in the Zn-tolerant isolate. The adaptive Zn tolerance in S. bovinus is therefore achieved by a preferential removal of Zn out of the cytoplasm, back into the apoplast, instead of the usual transfer of Zn into the vacuole. Zn exclusion in the fungal symbiont eventually contributes to a lower Zn influx in host plants.

(Less)
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Adaptation, Physiological, Basidiomycota/metabolism, Biological Transport/drug effects, Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology, Cell Wall/metabolism, Cytoplasm/metabolism, Kinetics, Mycelium/metabolism, Mycorrhizae/metabolism, Proton Ionophores/pharmacology, Soil Pollutants/metabolism, Vacuoles/metabolism, Zinc/metabolism
in
Metallomics
volume
5
issue
9
pages
9 pages
publisher
Royal Society of Chemistry
external identifiers
  • scopus:84882970218
ISSN
1756-5901
DOI
10.1039/c3mt00061c
language
English
LU publication?
no
id
67f30b2b-a9d5-40f3-b9a1-08422ef691d2
date added to LUP
2019-03-05 15:50:24
date last changed
2019-06-25 03:50:14
@article{67f30b2b-a9d5-40f3-b9a1-08422ef691d2,
  abstract     = {<p>On Zn-polluted soils, populations of the ectomycorrhizal basidiomycete Suillus bovinus exhibit an elevated Zn tolerance when compared to populations on non-polluted sites. To elucidate the mechanism of Zn tolerance, the time-course of Zn uptake was studied in isolates with contrasting Zn tolerance. Unidirectional fluxes and subcellular compartmentation of Zn were investigated through radiotracer flux analyses. Fluorescence imaging was used to support the subcellular Zn compartmentation. After 2 h of exposure to 200 μM Zn, significantly more Zn was accumulated in Zn-sensitive isolates compared to tolerant isolates, despite similar short-term uptake kinetics and similar extracellular Zn sequestration in cell walls. In Zn-sensitive isolates twice as much Zn accumulated in the cytoplasm and 12 times more Zn in the vacuole. (65)Zn efflux analyses revealed a considerably faster Zn export in the Zn-tolerant isolate. The adaptive Zn tolerance in S. bovinus is therefore achieved by a preferential removal of Zn out of the cytoplasm, back into the apoplast, instead of the usual transfer of Zn into the vacuole. Zn exclusion in the fungal symbiont eventually contributes to a lower Zn influx in host plants. </p>},
  author       = {Ruytinx, Joske and Nguyen, Hoai and Van Hees, May and Op De Beeck, Michiel and Vangronsveld, Jaco and Carleer, Robert and Colpaert, Jan V and Adriaensen, Kristin},
  issn         = {1756-5901},
  keyword      = {Adaptation, Physiological,Basidiomycota/metabolism,Biological Transport/drug effects,Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology,Cell Wall/metabolism,Cytoplasm/metabolism,Kinetics,Mycelium/metabolism,Mycorrhizae/metabolism,Proton Ionophores/pharmacology,Soil Pollutants/metabolism,Vacuoles/metabolism,Zinc/metabolism},
  language     = {eng},
  number       = {9},
  pages        = {33--1225},
  publisher    = {Royal Society of Chemistry},
  series       = {Metallomics},
  title        = {Zinc export results in adaptive zinc tolerance in the ectomycorrhizal basidiomycete Suillus bovinus},
  url          = {http://dx.doi.org/10.1039/c3mt00061c},
  volume       = {5},
  year         = {2013},
}