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Molecular characterization of marine cyanobacteria from the Indian subcontinent deduced from sequence analysis of the phycocyanin operon (cpcB-IGS-cpcA) and 16S-23S ITS region

Premanandh, Jagadeesan ; Priya, Balakrishnan ; Teneva, Ivanka ; Dzhambazov, Balik ; Prabaharan, Dharmar and Uma, Lakshmanan (2006) In Journal of Microbiology 44(6). p.607-616
Abstract
Molecular characterization of ten marine cyanobacterial isolates belonging to the order Oscillatoriales was carried out using the phycocyanin locus (cpcBA-IGS) and the 16S-23S internally transcribed spacer region. DNA sequences from the phycocyanin operon discriminated ten genotypes, which corresponded to seven morphotypes identified by traditional microscopic analysis. The cpcB coding region revealed 17% nucleotide variation, while cpcA exhibited 29% variation across the studied species. Phylogenetic analyses support the conclusion that the Phormidium and Leptolyngbya genera are not monophyletic. The nucleotide variations were heterogeneously distributed with no or minimal informative nucleotides. Our results suggest that the... (More)
Molecular characterization of ten marine cyanobacterial isolates belonging to the order Oscillatoriales was carried out using the phycocyanin locus (cpcBA-IGS) and the 16S-23S internally transcribed spacer region. DNA sequences from the phycocyanin operon discriminated ten genotypes, which corresponded to seven morphotypes identified by traditional microscopic analysis. The cpcB coding region revealed 17% nucleotide variation, while cpcA exhibited 29% variation across the studied species. Phylogenetic analyses support the conclusion that the Phormidium and Leptolyngbya genera are not monophyletic. The nucleotide variations were heterogeneously distributed with no or minimal informative nucleotides. Our results suggest that the discriminatory power of the phycocyanin region varies across the cyanobacterial species and strains. The DNA sequence analysis of the 16S-23S internally transcribed spacer region also supports the polyphyletic nature of the studied oscillatorian cyanobacteria. This study demonstrated that morphologically very similar strains might differ genotypically. Thus, molecular approaches comprising different gene regions in combination with morphological criteria may provide better taxonomical resolution of the order Oscillatoriales. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
phycocyanin operon, Phormidium, Leptolyngbya, ITS, IGS, cyanobacteria, genetic diversity, taxonomy, phylogeny
in
Journal of Microbiology
volume
44
issue
6
pages
607 - 616
publisher
The Microbiological Society of Korea
external identifiers
  • wos:000243214600004
  • scopus:33846942323
ISSN
1225-8873
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Medical Inflammation Research (013212019)
id
7bf50359-b47c-482a-802f-b4eaa987c6b4 (old id 680079)
alternative location
http://www.msk.or.kr/jsp/view_old_journalD.jsp?paperSeq=2467
date added to LUP
2016-04-01 12:09:18
date last changed
2021-08-25 02:14:40
@article{7bf50359-b47c-482a-802f-b4eaa987c6b4,
  abstract     = {Molecular characterization of ten marine cyanobacterial isolates belonging to the order Oscillatoriales was carried out using the phycocyanin locus (cpcBA-IGS) and the 16S-23S internally transcribed spacer region. DNA sequences from the phycocyanin operon discriminated ten genotypes, which corresponded to seven morphotypes identified by traditional microscopic analysis. The cpcB coding region revealed 17% nucleotide variation, while cpcA exhibited 29% variation across the studied species. Phylogenetic analyses support the conclusion that the Phormidium and Leptolyngbya genera are not monophyletic. The nucleotide variations were heterogeneously distributed with no or minimal informative nucleotides. Our results suggest that the discriminatory power of the phycocyanin region varies across the cyanobacterial species and strains. The DNA sequence analysis of the 16S-23S internally transcribed spacer region also supports the polyphyletic nature of the studied oscillatorian cyanobacteria. This study demonstrated that morphologically very similar strains might differ genotypically. Thus, molecular approaches comprising different gene regions in combination with morphological criteria may provide better taxonomical resolution of the order Oscillatoriales.},
  author       = {Premanandh, Jagadeesan and Priya, Balakrishnan and Teneva, Ivanka and Dzhambazov, Balik and Prabaharan, Dharmar and Uma, Lakshmanan},
  issn         = {1225-8873},
  language     = {eng},
  number       = {6},
  pages        = {607--616},
  publisher    = {The Microbiological Society of Korea},
  series       = {Journal of Microbiology},
  title        = {Molecular characterization of marine cyanobacteria from the Indian subcontinent deduced from sequence analysis of the phycocyanin operon (cpcB-IGS-cpcA) and 16S-23S ITS region},
  url          = {http://www.msk.or.kr/jsp/view_old_journalD.jsp?paperSeq=2467},
  volume       = {44},
  year         = {2006},
}