Molecular characterization of marine cyanobacteria from the Indian subcontinent deduced from sequence analysis of the phycocyanin operon (cpcB-IGS-cpcA) and 16S-23S ITS region
(2006) In Journal of Microbiology 44(6). p.607-616- Abstract
- Molecular characterization of ten marine cyanobacterial isolates belonging to the order Oscillatoriales was carried out using the phycocyanin locus (cpcBA-IGS) and the 16S-23S internally transcribed spacer region. DNA sequences from the phycocyanin operon discriminated ten genotypes, which corresponded to seven morphotypes identified by traditional microscopic analysis. The cpcB coding region revealed 17% nucleotide variation, while cpcA exhibited 29% variation across the studied species. Phylogenetic analyses support the conclusion that the Phormidium and Leptolyngbya genera are not monophyletic. The nucleotide variations were heterogeneously distributed with no or minimal informative nucleotides. Our results suggest that the... (More)
- Molecular characterization of ten marine cyanobacterial isolates belonging to the order Oscillatoriales was carried out using the phycocyanin locus (cpcBA-IGS) and the 16S-23S internally transcribed spacer region. DNA sequences from the phycocyanin operon discriminated ten genotypes, which corresponded to seven morphotypes identified by traditional microscopic analysis. The cpcB coding region revealed 17% nucleotide variation, while cpcA exhibited 29% variation across the studied species. Phylogenetic analyses support the conclusion that the Phormidium and Leptolyngbya genera are not monophyletic. The nucleotide variations were heterogeneously distributed with no or minimal informative nucleotides. Our results suggest that the discriminatory power of the phycocyanin region varies across the cyanobacterial species and strains. The DNA sequence analysis of the 16S-23S internally transcribed spacer region also supports the polyphyletic nature of the studied oscillatorian cyanobacteria. This study demonstrated that morphologically very similar strains might differ genotypically. Thus, molecular approaches comprising different gene regions in combination with morphological criteria may provide better taxonomical resolution of the order Oscillatoriales. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/680079
- author
- Premanandh, Jagadeesan ; Priya, Balakrishnan ; Teneva, Ivanka ; Dzhambazov, Balik LU ; Prabaharan, Dharmar and Uma, Lakshmanan
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- phycocyanin operon, Phormidium, Leptolyngbya, ITS, IGS, cyanobacteria, genetic diversity, taxonomy, phylogeny
- in
- Journal of Microbiology
- volume
- 44
- issue
- 6
- pages
- 607 - 616
- publisher
- The Microbiological Society of Korea
- external identifiers
-
- wos:000243214600004
- scopus:33846942323
- ISSN
- 1225-8873
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Medical Inflammation Research (013212019)
- id
- 7bf50359-b47c-482a-802f-b4eaa987c6b4 (old id 680079)
- alternative location
- http://www.msk.or.kr/jsp/view_old_journalD.jsp?paperSeq=2467
- date added to LUP
- 2016-04-01 12:09:18
- date last changed
- 2022-01-26 23:36:18
@article{7bf50359-b47c-482a-802f-b4eaa987c6b4,
abstract = {{Molecular characterization of ten marine cyanobacterial isolates belonging to the order Oscillatoriales was carried out using the phycocyanin locus (cpcBA-IGS) and the 16S-23S internally transcribed spacer region. DNA sequences from the phycocyanin operon discriminated ten genotypes, which corresponded to seven morphotypes identified by traditional microscopic analysis. The cpcB coding region revealed 17% nucleotide variation, while cpcA exhibited 29% variation across the studied species. Phylogenetic analyses support the conclusion that the Phormidium and Leptolyngbya genera are not monophyletic. The nucleotide variations were heterogeneously distributed with no or minimal informative nucleotides. Our results suggest that the discriminatory power of the phycocyanin region varies across the cyanobacterial species and strains. The DNA sequence analysis of the 16S-23S internally transcribed spacer region also supports the polyphyletic nature of the studied oscillatorian cyanobacteria. This study demonstrated that morphologically very similar strains might differ genotypically. Thus, molecular approaches comprising different gene regions in combination with morphological criteria may provide better taxonomical resolution of the order Oscillatoriales.}},
author = {{Premanandh, Jagadeesan and Priya, Balakrishnan and Teneva, Ivanka and Dzhambazov, Balik and Prabaharan, Dharmar and Uma, Lakshmanan}},
issn = {{1225-8873}},
keywords = {{phycocyanin operon; Phormidium; Leptolyngbya; ITS; IGS; cyanobacteria; genetic diversity; taxonomy; phylogeny}},
language = {{eng}},
number = {{6}},
pages = {{607--616}},
publisher = {{The Microbiological Society of Korea}},
series = {{Journal of Microbiology}},
title = {{Molecular characterization of marine cyanobacteria from the Indian subcontinent deduced from sequence analysis of the phycocyanin operon (cpcB-IGS-cpcA) and 16S-23S ITS region}},
url = {{http://www.msk.or.kr/jsp/view_old_journalD.jsp?paperSeq=2467}},
volume = {{44}},
year = {{2006}},
}