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Variation in the interaction between alleles of HvAPETALA2 and microRNA172 determines the density of grains on the barley inflorescence

Houston, K.; McKim, S. M.; Comadran, J.; Bonar, N.; Druka, I.; Uzrek, N.; Cirillo, E.; Guzy-Wrobelska, J.; Collins, N. C. and Halpin, C., et al. (2013) In Proceedings of the National Academy of Sciences 110(41). p.16675-16680
Abstract
Within the cereal grasses, variation in inflorescence architecture results in a conspicuous morphological diversity that in crop species influences the yield of cereal grains. Although significant progress has been made in identifying some of the genes underlying this variation in maize and rice, in the temperate cereals, a group that includes wheat, barley, and rye, only the dosage-dependent and highly pleiotropic Q locus in hexaploid wheat has been molecularly characterized. Here we show that the characteristic variation in the density of grains along the inflorescence, or spike, of modern cultivated barley (Hordeum vulgare) is largely the consequence of a perturbed interaction between microRNA172 and its corresponding binding site in... (More)
Within the cereal grasses, variation in inflorescence architecture results in a conspicuous morphological diversity that in crop species influences the yield of cereal grains. Although significant progress has been made in identifying some of the genes underlying this variation in maize and rice, in the temperate cereals, a group that includes wheat, barley, and rye, only the dosage-dependent and highly pleiotropic Q locus in hexaploid wheat has been molecularly characterized. Here we show that the characteristic variation in the density of grains along the inflorescence, or spike, of modern cultivated barley (Hordeum vulgare) is largely the consequence of a perturbed interaction between microRNA172 and its corresponding binding site in the mRNA of an APELATA2 (AP2)-like transcription factor, HvAP2. We used genome-wide association and biparental mapping to identify HvAP2. By comparing inflorescence development and HvAP2 transcript abundance in an extreme dense-spike mutant and its nearly isogenic WT line, we show that HvAP2 turnover driven by microRNA 172 regulates the length of a critical developmental window that is required for elongation of the inflorescence internodes. Our data indicate that this heterochronic change, an altered timing of developmental events caused by specific temporal variation in the efficiency of HvAP2 turnover, leads to the striking differences in the size and shape of the barley spike. (Less)
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Principal Component Analysis, Molecular Sequence Data, Scanning, Electron, Microscopy, MicroRNAs/genetics/*metabolism, Hordeum/*genetics/physiology, Genome-Wide Association Study, Flowers/genetics/*physiology/ultrastructure, Base Sequence, DNA Primers/genetics, Real-Time Polymerase Chain Reaction, Seeds/*physiology, Sequence Alignment, Sequence Analysis, DNA, Transcription Factors/genetics/*metabolism, genome wide association scan, heterochrony
in
Proceedings of the National Academy of Sciences
volume
110
issue
41
pages
16675 - 16680
publisher
National Acad Sciences
external identifiers
  • scopus:84885341442
ISSN
1091-6490
DOI
10.1073/pnas.1311681110
language
English
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no
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68031d25-bb6f-47f6-b02e-e5fa37650e0e (old id 8001665)
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http://www.ncbi.nlm.nih.gov/pubmed/24065816
date added to LUP
2015-09-30 14:26:46
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2019-06-16 03:20:44
@article{68031d25-bb6f-47f6-b02e-e5fa37650e0e,
  abstract     = {Within the cereal grasses, variation in inflorescence architecture results in a conspicuous morphological diversity that in crop species influences the yield of cereal grains. Although significant progress has been made in identifying some of the genes underlying this variation in maize and rice, in the temperate cereals, a group that includes wheat, barley, and rye, only the dosage-dependent and highly pleiotropic Q locus in hexaploid wheat has been molecularly characterized. Here we show that the characteristic variation in the density of grains along the inflorescence, or spike, of modern cultivated barley (Hordeum vulgare) is largely the consequence of a perturbed interaction between microRNA172 and its corresponding binding site in the mRNA of an APELATA2 (AP2)-like transcription factor, HvAP2. We used genome-wide association and biparental mapping to identify HvAP2. By comparing inflorescence development and HvAP2 transcript abundance in an extreme dense-spike mutant and its nearly isogenic WT line, we show that HvAP2 turnover driven by microRNA 172 regulates the length of a critical developmental window that is required for elongation of the inflorescence internodes. Our data indicate that this heterochronic change, an altered timing of developmental events caused by specific temporal variation in the efficiency of HvAP2 turnover, leads to the striking differences in the size and shape of the barley spike.},
  author       = {Houston, K. and McKim, S. M. and Comadran, J. and Bonar, N. and Druka, I. and Uzrek, N. and Cirillo, E. and Guzy-Wrobelska, J. and Collins, N. C. and Halpin, C. and Hansson, Mats and Dockter, C. and Druka, A. and Waugh, R.},
  issn         = {1091-6490},
  keyword      = {Principal Component Analysis,Molecular Sequence Data,Scanning,Electron,Microscopy,MicroRNAs/genetics/*metabolism,Hordeum/*genetics/physiology,Genome-Wide Association Study,Flowers/genetics/*physiology/ultrastructure,Base Sequence,DNA Primers/genetics,Real-Time Polymerase Chain Reaction,Seeds/*physiology,Sequence Alignment,Sequence Analysis,DNA,Transcription Factors/genetics/*metabolism,genome wide association scan,heterochrony},
  language     = {eng},
  number       = {41},
  pages        = {16675--16680},
  publisher    = {National Acad Sciences},
  series       = {Proceedings of the National Academy of Sciences},
  title        = {Variation in the interaction between alleles of HvAPETALA2 and microRNA172 determines the density of grains on the barley inflorescence},
  url          = {http://dx.doi.org/10.1073/pnas.1311681110},
  volume       = {110},
  year         = {2013},
}