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Pyrroloquinoline quinone glucose dehydrogenase adopted in thermometric analysis for enhancement of glucose determination

Xie, Weihong; Bülow, Leif LU and Xie, Bin LU (2018) In Journal of Thermal Analysis and Calorimetry p.1-7
Abstract

A broad measurement range of glucose is often required in clinical analysis, especially for diabetic patients where glucose levels can be very high. Pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) has previously been used in electrochemical quantification of glucose with an extended linear range. However, in real sample determination of glucose, interferences from electroactive substances in blood are unavoidable. Calorimetric biosensors, e.g., the Enzyme Thermistor, are insensitive to either directly electroactive or optical interferences often present in real clinical samples. This paper describes a novel analytical strategy where the intrinsic advantages of PQQGDH are combined with the Enzyme Thermistor as biosensor using... (More)

A broad measurement range of glucose is often required in clinical analysis, especially for diabetic patients where glucose levels can be very high. Pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) has previously been used in electrochemical quantification of glucose with an extended linear range. However, in real sample determination of glucose, interferences from electroactive substances in blood are unavoidable. Calorimetric biosensors, e.g., the Enzyme Thermistor, are insensitive to either directly electroactive or optical interferences often present in real clinical samples. This paper describes a novel analytical strategy where the intrinsic advantages of PQQGDH are combined with the Enzyme Thermistor as biosensor using calorimetric detection as general measurement principle. When compared with the most frequently used enzyme glucose oxidase, PQQGDH has a higher catalytic efficiency and is insensitive to the availability of oxygen. The use of calorimetry in this context resulted in a broad linear range of glucose measurements, from 0.009 to 100 mM, an excellent specificity and insignificant side effects of compounds present in blood at high concentrations, such as lactate and urea.

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author
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
Clinical analysis, Enzyme Thermistor (ET), Glucose, Pyrroloquinoline quinone glucose dehydrogenase, Thermal biosensor
in
Journal of Thermal Analysis and Calorimetry
pages
7 pages
publisher
Akademiai Kiado
external identifiers
  • scopus:85046026700
ISSN
1388-6150
DOI
10.1007/s10973-018-7273-0
language
English
LU publication?
yes
id
68358aa0-b7d4-4e60-8297-5b626df1cdab
date added to LUP
2018-05-14 13:33:55
date last changed
2018-06-12 15:30:30
@article{68358aa0-b7d4-4e60-8297-5b626df1cdab,
  abstract     = {<p>A broad measurement range of glucose is often required in clinical analysis, especially for diabetic patients where glucose levels can be very high. Pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) has previously been used in electrochemical quantification of glucose with an extended linear range. However, in real sample determination of glucose, interferences from electroactive substances in blood are unavoidable. Calorimetric biosensors, e.g., the Enzyme Thermistor, are insensitive to either directly electroactive or optical interferences often present in real clinical samples. This paper describes a novel analytical strategy where the intrinsic advantages of PQQGDH are combined with the Enzyme Thermistor as biosensor using calorimetric detection as general measurement principle. When compared with the most frequently used enzyme glucose oxidase, PQQGDH has a higher catalytic efficiency and is insensitive to the availability of oxygen. The use of calorimetry in this context resulted in a broad linear range of glucose measurements, from 0.009 to 100 mM, an excellent specificity and insignificant side effects of compounds present in blood at high concentrations, such as lactate and urea.</p>},
  author       = {Xie, Weihong and Bülow, Leif and Xie, Bin},
  issn         = {1388-6150},
  keyword      = {Clinical analysis,Enzyme Thermistor (ET),Glucose,Pyrroloquinoline quinone glucose dehydrogenase,Thermal biosensor},
  language     = {eng},
  month        = {04},
  pages        = {1--7},
  publisher    = {Akademiai Kiado},
  series       = {Journal of Thermal Analysis and Calorimetry},
  title        = {Pyrroloquinoline quinone glucose dehydrogenase adopted in thermometric analysis for enhancement of glucose determination},
  url          = {http://dx.doi.org/10.1007/s10973-018-7273-0},
  year         = {2018},
}