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Improved preparation technique of cervical carcinoma for flow cytometric DNA analysis with tissue disintegration in hydrochloric acid

Fernö, Mårten LU ; Långström-Einarsson, Eva LU ; Willen, R ; Baldetorp, Bo LU and Killander, Dick LU (1990) In In Vivo 4(4). p.225-229
Abstract
An one-step procedure using a nuclear isolation medium containing propidium iodide has been found to be a suitable preparation technique for flow cytometric DNA analysis in breast cancer samples. In the case of cervical squamous carcinoma, a pretreatment with HCl seems to be a methodological improvement. One advantage with the HCl modification is that some "false" near-diploid cell populations are abolished. These "false" G0/G1 peaks may represent diploid nuclei with a different stainability for propidium iodide compared to normal diploid nuclei. The HCl treatment has, furthermore, the advantage of increasing the elution of nuclei (mean factor of 4.0), especially non-diploid nuclei from higher differentiated squamous carcinomas.
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
In Vivo
volume
4
issue
4
pages
225 - 229
publisher
International Institute of Anticancer Research
external identifiers
  • pmid:2103364
  • scopus:0025200599
ISSN
0258-851X
language
English
LU publication?
yes
id
68523bba-6fe1-4bbf-8cab-e6e2d8e6688d (old id 1105006)
date added to LUP
2016-04-01 15:36:25
date last changed
2025-10-14 10:43:28
@article{68523bba-6fe1-4bbf-8cab-e6e2d8e6688d,
  abstract     = {{An one-step procedure using a nuclear isolation medium containing propidium iodide has been found to be a suitable preparation technique for flow cytometric DNA analysis in breast cancer samples. In the case of cervical squamous carcinoma, a pretreatment with HCl seems to be a methodological improvement. One advantage with the HCl modification is that some "false" near-diploid cell populations are abolished. These "false" G0/G1 peaks may represent diploid nuclei with a different stainability for propidium iodide compared to normal diploid nuclei. The HCl treatment has, furthermore, the advantage of increasing the elution of nuclei (mean factor of 4.0), especially non-diploid nuclei from higher differentiated squamous carcinomas.}},
  author       = {{Fernö, Mårten and Långström-Einarsson, Eva and Willen, R and Baldetorp, Bo and Killander, Dick}},
  issn         = {{0258-851X}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{225--229}},
  publisher    = {{International Institute of Anticancer Research}},
  series       = {{In Vivo}},
  title        = {{Improved preparation technique of cervical carcinoma for flow cytometric DNA analysis with tissue disintegration in hydrochloric acid}},
  volume       = {{4}},
  year         = {{1990}},
}