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Are there generic mechanisms governing interactions between nanoparticles and cells? Epitope mapping the outer layer of the protein-material interface

Lynch, Iseult LU (2007) In Physica A: Statistical Mechanics and its Applications 373. p.511-520
Abstract
In this paper we discuss the possibility of a general paradigm for cell-biomaterial and cell-nanoparticle interactions. The basis of the paradigm is that the nature of the biomaterial or nanoparticle surface is not the important parameter, but rather the nature of the outermost layer of adsorbed proteins as well as long-lived misfolded proteins shed from the surfaces. If the adsorbed protein is irreversibly adsorbed onto the surface it may be sufficiently disrupted so that a variety of peptide units (here termed "cryptic epitopes") not usually expressed in nature at the surface of the protein become exposed. Similarly, where there is a slow exchange time with the surface, surface-induced perturbations may lead to long-lived misfolded... (More)
In this paper we discuss the possibility of a general paradigm for cell-biomaterial and cell-nanoparticle interactions. The basis of the paradigm is that the nature of the biomaterial or nanoparticle surface is not the important parameter, but rather the nature of the outermost layer of adsorbed proteins as well as long-lived misfolded proteins shed from the surfaces. If the adsorbed protein is irreversibly adsorbed onto the surface it may be sufficiently disrupted so that a variety of peptide units (here termed "cryptic epitopes") not usually expressed in nature at the surface of the protein become exposed. Similarly, where there is a slow exchange time with the surface, surface-induced perturbations may lead to long-lived misfolded proteins being shed from the surface and continuing to express altered surface peptide sequences. In cases where the proteins have lost most of their tertiary structure, anomalous peptide sequences and geometries that are not displayed at the surface by the native protein may in fact be presented after surface adsorption of a protein. Such anomalous surface expressions could contain novel epitopes that trigger various signalling pathways or even diseases. Thus, future approaches to understanding cell-biomaterial and cell-nanoparticle interactions should focus on characterising the outer layer of the adsorbed proteins, or "epitope mapping" as well as examining the possibility of formation of essentially "new" proteins as a result of desorption of conformationally or geometrically altered proteins. (c) 2006 Published by Elsevier B.V. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
nanoparticles, surface protein layer, epitope mapping, protein adsorption, biomaterial-cell interaction
in
Physica A: Statistical Mechanics and its Applications
volume
373
pages
511 - 520
publisher
Elsevier
external identifiers
  • wos:000242316000044
  • scopus:33750440502
ISSN
0378-4371
DOI
10.1016/j.physa.2006.06.008
language
English
LU publication?
yes
id
ef44d152-88c7-43ac-9fd2-007ccaa2670d (old id 685430)
date added to LUP
2007-12-14 14:29:33
date last changed
2017-11-19 04:13:52
@article{ef44d152-88c7-43ac-9fd2-007ccaa2670d,
  abstract     = {In this paper we discuss the possibility of a general paradigm for cell-biomaterial and cell-nanoparticle interactions. The basis of the paradigm is that the nature of the biomaterial or nanoparticle surface is not the important parameter, but rather the nature of the outermost layer of adsorbed proteins as well as long-lived misfolded proteins shed from the surfaces. If the adsorbed protein is irreversibly adsorbed onto the surface it may be sufficiently disrupted so that a variety of peptide units (here termed "cryptic epitopes") not usually expressed in nature at the surface of the protein become exposed. Similarly, where there is a slow exchange time with the surface, surface-induced perturbations may lead to long-lived misfolded proteins being shed from the surface and continuing to express altered surface peptide sequences. In cases where the proteins have lost most of their tertiary structure, anomalous peptide sequences and geometries that are not displayed at the surface by the native protein may in fact be presented after surface adsorption of a protein. Such anomalous surface expressions could contain novel epitopes that trigger various signalling pathways or even diseases. Thus, future approaches to understanding cell-biomaterial and cell-nanoparticle interactions should focus on characterising the outer layer of the adsorbed proteins, or "epitope mapping" as well as examining the possibility of formation of essentially "new" proteins as a result of desorption of conformationally or geometrically altered proteins. (c) 2006 Published by Elsevier B.V.},
  author       = {Lynch, Iseult},
  issn         = {0378-4371},
  keyword      = {nanoparticles,surface protein layer,epitope mapping,protein adsorption,biomaterial-cell interaction},
  language     = {eng},
  pages        = {511--520},
  publisher    = {Elsevier},
  series       = {Physica A: Statistical Mechanics and its Applications},
  title        = {Are there generic mechanisms governing interactions between nanoparticles and cells? Epitope mapping the outer layer of the protein-material interface},
  url          = {http://dx.doi.org/10.1016/j.physa.2006.06.008},
  volume       = {373},
  year         = {2007},
}