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Expression of MMP-10 in lung cancer

Zhang, Xiaoying; Zhu, Shaowei; Luo, Guanghua; Zheng, Lu; Wei, Jiang; Zhu, Jiang; Mu, Qingfeng and Xu, Ning LU (2007) In Anticancer Research 27(4C). p.2791-2795
Abstract
Background: Matrix metalloproteinase (MMP)-mediated degradation of the extracellular matrix is a key point in tumor development and expansion. MMP-10 is one of the most important and well-characterized members of the MMP family. In the present study, we examined MMP-10 mRNA and protein levels in non-small cell lung cancer (NSCLC). Patients and Methods: Three endogenous reference genes including GAPDH, beta-actin and 18S rRNA, and MMP-10 mRNA levels were determined using real-time RT-PCR. Immunohistochemical staining was applied to examine MMP-10 protein levels. Both tumor and adjacent normal lung tissues were collected from 32 NSCLC patients. The mRNA levels of GAPDH, beta-actin and 18S rRNA exhibited great differences in tumor tissues and... (More)
Background: Matrix metalloproteinase (MMP)-mediated degradation of the extracellular matrix is a key point in tumor development and expansion. MMP-10 is one of the most important and well-characterized members of the MMP family. In the present study, we examined MMP-10 mRNA and protein levels in non-small cell lung cancer (NSCLC). Patients and Methods: Three endogenous reference genes including GAPDH, beta-actin and 18S rRNA, and MMP-10 mRNA levels were determined using real-time RT-PCR. Immunohistochemical staining was applied to examine MMP-10 protein levels. Both tumor and adjacent normal lung tissues were collected from 32 NSCLC patients. The mRNA levels of GAPDH, beta-actin and 18S rRNA exhibited great differences in tumor tissues and in the adjacent normal tissues. The ratio of mRNA levels in the tumor tissues compared to the adjacent normal tissues followed the pattern GAPDH > beta-actin > 18S rRNA. Thereafter, we chose 18S rRNA as the reference gene for MMP-10 mRNA level determinations. MMP-10 mRNA levels in tumor tissues were significantly lower than those in the adjacent normal tissues (p = 0.0423). However, the MMP- 10 protein levels were higher in the tumor tissues than in the adjacent normal tissues (p=0.0055). The MMP-10 mRNA level was positively-correlated to the MMP-10 protein level in tumor tissues (r=0.4672, p=0.0161), but this correlation was not seen in the adjacent normal tissues (r=-0.0030, p=0.9891). Conclusion: There were no statistical differences in MMP-10 mRNA levels and protein levels in relation to patient's gender, age, tumor stages, tumor size, lymph node metastasis or tumor histological type. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
real-time RT-PCR, endogenous reference gene, MMP-10, NSCLC, immunohistochemistry
in
Anticancer Research
volume
27
issue
4C
pages
2791 - 2795
publisher
International Institute of Cancer Research
external identifiers
  • wos:000248546100034
  • scopus:34547784153
ISSN
1791-7530
language
English
LU publication?
yes
id
179e4c31-b78d-41b3-9f85-af01fab8c280 (old id 686802)
date added to LUP
2007-12-19 11:28:22
date last changed
2017-08-20 03:28:34
@article{179e4c31-b78d-41b3-9f85-af01fab8c280,
  abstract     = {Background: Matrix metalloproteinase (MMP)-mediated degradation of the extracellular matrix is a key point in tumor development and expansion. MMP-10 is one of the most important and well-characterized members of the MMP family. In the present study, we examined MMP-10 mRNA and protein levels in non-small cell lung cancer (NSCLC). Patients and Methods: Three endogenous reference genes including GAPDH, beta-actin and 18S rRNA, and MMP-10 mRNA levels were determined using real-time RT-PCR. Immunohistochemical staining was applied to examine MMP-10 protein levels. Both tumor and adjacent normal lung tissues were collected from 32 NSCLC patients. The mRNA levels of GAPDH, beta-actin and 18S rRNA exhibited great differences in tumor tissues and in the adjacent normal tissues. The ratio of mRNA levels in the tumor tissues compared to the adjacent normal tissues followed the pattern GAPDH > beta-actin > 18S rRNA. Thereafter, we chose 18S rRNA as the reference gene for MMP-10 mRNA level determinations. MMP-10 mRNA levels in tumor tissues were significantly lower than those in the adjacent normal tissues (p = 0.0423). However, the MMP- 10 protein levels were higher in the tumor tissues than in the adjacent normal tissues (p=0.0055). The MMP-10 mRNA level was positively-correlated to the MMP-10 protein level in tumor tissues (r=0.4672, p=0.0161), but this correlation was not seen in the adjacent normal tissues (r=-0.0030, p=0.9891). Conclusion: There were no statistical differences in MMP-10 mRNA levels and protein levels in relation to patient's gender, age, tumor stages, tumor size, lymph node metastasis or tumor histological type.},
  author       = {Zhang, Xiaoying and Zhu, Shaowei and Luo, Guanghua and Zheng, Lu and Wei, Jiang and Zhu, Jiang and Mu, Qingfeng and Xu, Ning},
  issn         = {1791-7530},
  keyword      = {real-time RT-PCR,endogenous reference gene,MMP-10,NSCLC,immunohistochemistry},
  language     = {eng},
  number       = {4C},
  pages        = {2791--2795},
  publisher    = {International Institute of Cancer Research},
  series       = {Anticancer Research},
  title        = {Expression of MMP-10 in lung cancer},
  volume       = {27},
  year         = {2007},
}