Anti-D (WinRho SD™) treatment of children with chronic autoimmune thrombocytopenic purpura stimulates transient cytokine/chemokine production
(2002) In American Journal of Hematology 69(3). p.225-227- Abstract
Intravenous anti-D is often used in the treatment of autoimmune thrombocytopenic purpura (AITP), but little is known about its mechanisms of action. To investigate anti-D's potential in vivo mechanism(s) of action, a small group (N = 7) of children with chronic AITP was studied. The children initially received either 25 or 50 μg/kg of WinRho-SD in a four-cycle cross-over trial, and peripheral blood samples from the first and third cycles were assessed for cytokine levels at pre-treatment, 3 hr, 1 day, and 8 days post-treatment. Results showed that platelet counts significantly increased in all the children by day 8 post-treatment. Analysis of serum by ELISA showed that there was a significant but transient rise in both pro- and... (More)
Intravenous anti-D is often used in the treatment of autoimmune thrombocytopenic purpura (AITP), but little is known about its mechanisms of action. To investigate anti-D's potential in vivo mechanism(s) of action, a small group (N = 7) of children with chronic AITP was studied. The children initially received either 25 or 50 μg/kg of WinRho-SD in a four-cycle cross-over trial, and peripheral blood samples from the first and third cycles were assessed for cytokine levels at pre-treatment, 3 hr, 1 day, and 8 days post-treatment. Results showed that platelet counts significantly increased in all the children by day 8 post-treatment. Analysis of serum by ELISA showed that there was a significant but transient rise in both pro- and anti-inflammatory cytokine/chemokine levels (e.g., IL1RA, IL6, GM-CSF, MCP-1α, TNF-α and MCP-1) by 3 hr post-treatment in both cycles which returned to baseline levels by 8 days post-treatment. These results suggest that anti-D administration may initially activate the RES in the form of cytokine/chemokine secretion, which is subsequently followed by an increase in platelet counts. It is possible that the induced cytokine/chemokine storm may have an effect on several physiological processes such as those mediating either adverse effects or potentially RES phagocytic activity.
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- author
- Semple, J. W. LU ; Allen, D. ; Rutherford, M. ; Woloski, M. ; David, M. ; Wakefield, C. ; Butchart, S. ; Freedman, J. and Blanchette, V.
- publishing date
- 2002-03-09
- type
- Contribution to journal
- publication status
- published
- keywords
- Anti-D, Autoimmune thrombocytopenia, Cytokines
- in
- American Journal of Hematology
- volume
- 69
- issue
- 3
- pages
- 225 - 227
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:11891813
- scopus:0036182846
- ISSN
- 0361-8609
- DOI
- 10.1002/ajh.10065
- language
- English
- LU publication?
- no
- id
- 688051d9-d350-4751-bb78-5df3f648fb80
- date added to LUP
- 2019-12-03 10:25:39
- date last changed
- 2024-05-30 07:42:07
@article{688051d9-d350-4751-bb78-5df3f648fb80,
abstract = {{<p>Intravenous anti-D is often used in the treatment of autoimmune thrombocytopenic purpura (AITP), but little is known about its mechanisms of action. To investigate anti-D's potential in vivo mechanism(s) of action, a small group (N = 7) of children with chronic AITP was studied. The children initially received either 25 or 50 μg/kg of WinRho-SD in a four-cycle cross-over trial, and peripheral blood samples from the first and third cycles were assessed for cytokine levels at pre-treatment, 3 hr, 1 day, and 8 days post-treatment. Results showed that platelet counts significantly increased in all the children by day 8 post-treatment. Analysis of serum by ELISA showed that there was a significant but transient rise in both pro- and anti-inflammatory cytokine/chemokine levels (e.g., IL1RA, IL6, GM-CSF, MCP-1α, TNF-α and MCP-1) by 3 hr post-treatment in both cycles which returned to baseline levels by 8 days post-treatment. These results suggest that anti-D administration may initially activate the RES in the form of cytokine/chemokine secretion, which is subsequently followed by an increase in platelet counts. It is possible that the induced cytokine/chemokine storm may have an effect on several physiological processes such as those mediating either adverse effects or potentially RES phagocytic activity.</p>}},
author = {{Semple, J. W. and Allen, D. and Rutherford, M. and Woloski, M. and David, M. and Wakefield, C. and Butchart, S. and Freedman, J. and Blanchette, V.}},
issn = {{0361-8609}},
keywords = {{Anti-D; Autoimmune thrombocytopenia; Cytokines}},
language = {{eng}},
month = {{03}},
number = {{3}},
pages = {{225--227}},
publisher = {{John Wiley & Sons Inc.}},
series = {{American Journal of Hematology}},
title = {{Anti-D (WinRho SD™) treatment of children with chronic autoimmune thrombocytopenic purpura stimulates transient cytokine/chemokine production}},
url = {{http://dx.doi.org/10.1002/ajh.10065}},
doi = {{10.1002/ajh.10065}},
volume = {{69}},
year = {{2002}},
}