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Characterization of major enzymes and genes involved in flavonoid and proanthocyanidin biosynthesis during fruit development in strawberry (Fragaria x ananassa)

Almeida, Joao LU ; D'Amico, Eleonora; Preuss, Anja; Carbone, Fabrizio; de Vos, C. H. Ric; Deiml, Bettina; Mourgues, Fabienne; Perrotta, Gaetano; Fischer, Thilo C. and Bovy, Arnaud G., et al. (2007) In Archives of Biochemistry and Biophysics 465(1). p.61-71
Abstract
The biosynthesis of flavonoids and proanthocyanidins was studied in cultivated strawberry (Fragaria x ananassa) by combining biochemical and molecular approaches. Chemical analyses showed that ripe strawberries accumulate high amounts of pelargonidin-derived anthocyanins, and a larger pool of 3',4'-hydroxylated proanthocyanidins. Activities and properties of major recombinant enzymes were demonstrated by means of in vitro assays, with special emphasis on specificity for the biologically relevant 4'- and 3',4'-hydroxylated compounds. Only leucoanthocyanidin reductase showed a strict specificity for the 3',4'-hydroxylated leucocyanidin, while other enzymes accepted either hydroxylated substrate with different relative activity rates. The... (More)
The biosynthesis of flavonoids and proanthocyanidins was studied in cultivated strawberry (Fragaria x ananassa) by combining biochemical and molecular approaches. Chemical analyses showed that ripe strawberries accumulate high amounts of pelargonidin-derived anthocyanins, and a larger pool of 3',4'-hydroxylated proanthocyanidins. Activities and properties of major recombinant enzymes were demonstrated by means of in vitro assays, with special emphasis on specificity for the biologically relevant 4'- and 3',4'-hydroxylated compounds. Only leucoanthocyanidin reductase showed a strict specificity for the 3',4'-hydroxylated leucocyanidin, while other enzymes accepted either hydroxylated substrate with different relative activity rates. The structure of late flavonoid pathway genes, leading to the synthesis of major compounds in ripe fruits, was elucidated. Complex developmental and spatial expression patterns were shown for phenylpropanoid and flavonoid genes in fruits throughout ripening as well as in leaves, petals and roots. Presented results elucidate key steps in the biosynthesis of strawberry flavonoid end products. (c) 2007 Elsevier Inc. All rights reserved. (Less)
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keywords
developmental gene expression, proanthocyanidins, recombinant enzyme activity, strawberry, preference, substrate, flavonoids, fruit ripening
in
Archives of Biochemistry and Biophysics
volume
465
issue
1
pages
61 - 71
publisher
Academic Press
external identifiers
  • wos:000249176400008
  • scopus:34548076817
ISSN
0003-9861
DOI
10.1016/j.abb.2007.04.040
language
English
LU publication?
yes
id
8d5ad169-9a7a-4f9f-99a9-08027816d8ba (old id 688424)
date added to LUP
2007-12-04 18:12:24
date last changed
2017-11-12 03:27:24
@article{8d5ad169-9a7a-4f9f-99a9-08027816d8ba,
  abstract     = {The biosynthesis of flavonoids and proanthocyanidins was studied in cultivated strawberry (Fragaria x ananassa) by combining biochemical and molecular approaches. Chemical analyses showed that ripe strawberries accumulate high amounts of pelargonidin-derived anthocyanins, and a larger pool of 3',4'-hydroxylated proanthocyanidins. Activities and properties of major recombinant enzymes were demonstrated by means of in vitro assays, with special emphasis on specificity for the biologically relevant 4'- and 3',4'-hydroxylated compounds. Only leucoanthocyanidin reductase showed a strict specificity for the 3',4'-hydroxylated leucocyanidin, while other enzymes accepted either hydroxylated substrate with different relative activity rates. The structure of late flavonoid pathway genes, leading to the synthesis of major compounds in ripe fruits, was elucidated. Complex developmental and spatial expression patterns were shown for phenylpropanoid and flavonoid genes in fruits throughout ripening as well as in leaves, petals and roots. Presented results elucidate key steps in the biosynthesis of strawberry flavonoid end products. (c) 2007 Elsevier Inc. All rights reserved.},
  author       = {Almeida, Joao and D'Amico, Eleonora and Preuss, Anja and Carbone, Fabrizio and de Vos, C. H. Ric and Deiml, Bettina and Mourgues, Fabienne and Perrotta, Gaetano and Fischer, Thilo C. and Bovy, Arnaud G. and Martens, Stefan and Rosati, Carlo},
  issn         = {0003-9861},
  keyword      = {developmental gene expression,proanthocyanidins,recombinant enzyme activity,strawberry,preference,substrate,flavonoids,fruit ripening},
  language     = {eng},
  number       = {1},
  pages        = {61--71},
  publisher    = {Academic Press},
  series       = {Archives of Biochemistry and Biophysics},
  title        = {Characterization of major enzymes and genes involved in flavonoid and proanthocyanidin biosynthesis during fruit development in strawberry (Fragaria x ananassa)},
  url          = {http://dx.doi.org/10.1016/j.abb.2007.04.040},
  volume       = {465},
  year         = {2007},
}