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Capacitive biosensor for detection of endotoxin

Limbut, Warakorn LU ; Hedström, Martin LU ; Thavarungkul, Panote; Kanatharana, Proespichaya and Mattiasson, Bo LU (2007) In Analytical and Bioanalytical Chemistry 389(2). p.517-525
Abstract
A capacitive biosensor for the detection of bacterial endotoxin has been developed. Endotoxin-neutralizing protein derived from American horseshoe crab was immobilized to a self-assembled thiol layer on a biosensor transducer (Au). Upon injection of a sample containing endotoxin, a decrease in the observed capacitive signal was registered. Endotoxin could be determined under optimum conditions with a detection limit of 1.0 x 10(-13) M and linearity ranging from 1.0 x 10(-13) to 1.0 x 10(-10) M. Good agreement was achieved when applying endotoxin preparations purified from an Escherichia coli cultivation to the capacitive biosensor system, utilizing the conventional method for quantitative endotoxin determination, the Limulus amebocyte... (More)
A capacitive biosensor for the detection of bacterial endotoxin has been developed. Endotoxin-neutralizing protein derived from American horseshoe crab was immobilized to a self-assembled thiol layer on a biosensor transducer (Au). Upon injection of a sample containing endotoxin, a decrease in the observed capacitive signal was registered. Endotoxin could be determined under optimum conditions with a detection limit of 1.0 x 10(-13) M and linearity ranging from 1.0 x 10(-13) to 1.0 x 10(-10) M. Good agreement was achieved when applying endotoxin preparations purified from an Escherichia coli cultivation to the capacitive biosensor system, utilizing the conventional method for quantitative endotoxin determination, the Limulus amebocyte lysate test as a reference. The capacitive biosensor method was statistically tested with the Wilcoxon signed rank test, which proved the system is acceptable for the quantitative analysis of bacterial endotoxin (P < 0.05). (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
monolayer, self-assembled monolayer, Limulus, capacitive biosensor, endotoxin
in
Analytical and Bioanalytical Chemistry
volume
389
issue
2
pages
517 - 525
publisher
Springer
external identifiers
  • wos:000249008200019
  • scopus:34548257739
ISSN
1618-2642
DOI
10.1007/s00216-007-1443-4
language
English
LU publication?
yes
id
d1acf08f-775f-416b-a0fe-8f78c688e9af (old id 688482)
date added to LUP
2007-12-14 14:52:04
date last changed
2017-11-05 03:49:53
@article{d1acf08f-775f-416b-a0fe-8f78c688e9af,
  abstract     = {A capacitive biosensor for the detection of bacterial endotoxin has been developed. Endotoxin-neutralizing protein derived from American horseshoe crab was immobilized to a self-assembled thiol layer on a biosensor transducer (Au). Upon injection of a sample containing endotoxin, a decrease in the observed capacitive signal was registered. Endotoxin could be determined under optimum conditions with a detection limit of 1.0 x 10(-13) M and linearity ranging from 1.0 x 10(-13) to 1.0 x 10(-10) M. Good agreement was achieved when applying endotoxin preparations purified from an Escherichia coli cultivation to the capacitive biosensor system, utilizing the conventional method for quantitative endotoxin determination, the Limulus amebocyte lysate test as a reference. The capacitive biosensor method was statistically tested with the Wilcoxon signed rank test, which proved the system is acceptable for the quantitative analysis of bacterial endotoxin (P &lt; 0.05).},
  author       = {Limbut, Warakorn and Hedström, Martin and Thavarungkul, Panote and Kanatharana, Proespichaya and Mattiasson, Bo},
  issn         = {1618-2642},
  keyword      = {monolayer,self-assembled monolayer,Limulus,capacitive biosensor,endotoxin},
  language     = {eng},
  number       = {2},
  pages        = {517--525},
  publisher    = {Springer},
  series       = {Analytical and Bioanalytical Chemistry},
  title        = {Capacitive biosensor for detection of endotoxin},
  url          = {http://dx.doi.org/10.1007/s00216-007-1443-4},
  volume       = {389},
  year         = {2007},
}