Capacitive biosensor for detection of endotoxin
(2007) In Analytical and Bioanalytical Chemistry 389(2). p.517-525- Abstract
- A capacitive biosensor for the detection of bacterial endotoxin has been developed. Endotoxin-neutralizing protein derived from American horseshoe crab was immobilized to a self-assembled thiol layer on a biosensor transducer (Au). Upon injection of a sample containing endotoxin, a decrease in the observed capacitive signal was registered. Endotoxin could be determined under optimum conditions with a detection limit of 1.0 x 10(-13) M and linearity ranging from 1.0 x 10(-13) to 1.0 x 10(-10) M. Good agreement was achieved when applying endotoxin preparations purified from an Escherichia coli cultivation to the capacitive biosensor system, utilizing the conventional method for quantitative endotoxin determination, the Limulus amebocyte... (More)
- A capacitive biosensor for the detection of bacterial endotoxin has been developed. Endotoxin-neutralizing protein derived from American horseshoe crab was immobilized to a self-assembled thiol layer on a biosensor transducer (Au). Upon injection of a sample containing endotoxin, a decrease in the observed capacitive signal was registered. Endotoxin could be determined under optimum conditions with a detection limit of 1.0 x 10(-13) M and linearity ranging from 1.0 x 10(-13) to 1.0 x 10(-10) M. Good agreement was achieved when applying endotoxin preparations purified from an Escherichia coli cultivation to the capacitive biosensor system, utilizing the conventional method for quantitative endotoxin determination, the Limulus amebocyte lysate test as a reference. The capacitive biosensor method was statistically tested with the Wilcoxon signed rank test, which proved the system is acceptable for the quantitative analysis of bacterial endotoxin (P < 0.05). (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/688482
- author
- Limbut, Warakorn LU ; Hedström, Martin LU ; Thavarungkul, Panote ; Kanatharana, Proespichaya and Mattiasson, Bo LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- monolayer, self-assembled monolayer, Limulus, capacitive biosensor, endotoxin
- in
- Analytical and Bioanalytical Chemistry
- volume
- 389
- issue
- 2
- pages
- 517 - 525
- publisher
- Springer
- external identifiers
-
- wos:000249008200019
- scopus:34548257739
- ISSN
- 1618-2642
- DOI
- 10.1007/s00216-007-1443-4
- language
- English
- LU publication?
- yes
- id
- d1acf08f-775f-416b-a0fe-8f78c688e9af (old id 688482)
- date added to LUP
- 2016-04-01 12:38:30
- date last changed
- 2022-03-29 03:41:23
@article{d1acf08f-775f-416b-a0fe-8f78c688e9af,
abstract = {{A capacitive biosensor for the detection of bacterial endotoxin has been developed. Endotoxin-neutralizing protein derived from American horseshoe crab was immobilized to a self-assembled thiol layer on a biosensor transducer (Au). Upon injection of a sample containing endotoxin, a decrease in the observed capacitive signal was registered. Endotoxin could be determined under optimum conditions with a detection limit of 1.0 x 10(-13) M and linearity ranging from 1.0 x 10(-13) to 1.0 x 10(-10) M. Good agreement was achieved when applying endotoxin preparations purified from an Escherichia coli cultivation to the capacitive biosensor system, utilizing the conventional method for quantitative endotoxin determination, the Limulus amebocyte lysate test as a reference. The capacitive biosensor method was statistically tested with the Wilcoxon signed rank test, which proved the system is acceptable for the quantitative analysis of bacterial endotoxin (P < 0.05).}},
author = {{Limbut, Warakorn and Hedström, Martin and Thavarungkul, Panote and Kanatharana, Proespichaya and Mattiasson, Bo}},
issn = {{1618-2642}},
keywords = {{monolayer; self-assembled monolayer; Limulus; capacitive biosensor; endotoxin}},
language = {{eng}},
number = {{2}},
pages = {{517--525}},
publisher = {{Springer}},
series = {{Analytical and Bioanalytical Chemistry}},
title = {{Capacitive biosensor for detection of endotoxin}},
url = {{http://dx.doi.org/10.1007/s00216-007-1443-4}},
doi = {{10.1007/s00216-007-1443-4}},
volume = {{389}},
year = {{2007}},
}