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Non-pylori Helicobacteraceae in the upper digestive tract of asymptomatic venezuelan subjects: Detection of helicobacter cetorum-like and candidatus Wolinella africanus-like DNA

Garcia-Amado, M. Alexandra; Abu Al-Soud, Waleed LU ; Borges-Landaez, Pedro; Contreras, Monica; Cedeno, Samandhy; Baez-Ramirez, Estalina; Dominguez-Bello, M. Gloria; Wadström, Torkel LU and Gueneau, Pulcherie (2007) In Helicobacter 12(5). p.553-558
Abstract
Background: The spectrum of human non-pylori Helicobacter infections is expanding, with species such as H. heilmannii and H. felis occasionally being associated with gastritis. However, the existence of non-pylori Helicobacter colonization in asymptomatic subjects has not been evaluated. The aim of this study was to investigate whether Helicobacter species other than pylori are present in the upper digestive tract of asymptomatic human subjects. Materials and methods: A Helicobacteraceae-specific semi-nested polymerase chain reaction (PCR) assay was used to detect Helicobacter-like organisms in the upper digestive tract of 91 Venezuelan volunteers (aged 18-68 years, 41 females, 50 males). Species were identified by denaturing gradient gel... (More)
Background: The spectrum of human non-pylori Helicobacter infections is expanding, with species such as H. heilmannii and H. felis occasionally being associated with gastritis. However, the existence of non-pylori Helicobacter colonization in asymptomatic subjects has not been evaluated. The aim of this study was to investigate whether Helicobacter species other than pylori are present in the upper digestive tract of asymptomatic human subjects. Materials and methods: A Helicobacteraceae-specific semi-nested polymerase chain reaction (PCR) assay was used to detect Helicobacter-like organisms in the upper digestive tract of 91 Venezuelan volunteers (aged 18-68 years, 41 females, 50 males). Species were identified by denaturing gradient gel electrophoresis analysis and sequencing of the PCR products. Results: We detected DNA sharing 99-100% sequence identity in over 300-400 bp with the 16S rRNA genes of H. pylori, H. cetorum, and Candidatus Wolinella africanus in 76%, 16%, and 15% of the subjects, respectively. Multiple colonization was documented in 10% of the subjects: H. cetorum and Candidatus W. africanus (4%), H. pylori and Candidatus W. africanus (4%), and H. pylori and H. cetorum (2%). Conclusions: Our results suggest that non-pylori Helicobacteraceae colonization is relatively common in the Venezuelan asymptomatic population. This is the first report documenting the presence of H. cetorum DNA in the human upper digestive tract, and the second report of the recently discovered Candidatus W. africanus. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
PCR, Helicobacteraceae, prevalence
in
Helicobacter
volume
12
issue
5
pages
553 - 558
publisher
Wiley-Blackwell
external identifiers
  • wos:000248996100011
  • scopus:34548163134
ISSN
1083-4389
DOI
10.1111/j.1523-5378.2007.00526.x
language
English
LU publication?
yes
id
3e7a9ab6-b949-4b65-97df-f650398a6e44 (old id 689027)
date added to LUP
2007-12-11 10:28:11
date last changed
2017-10-01 03:38:22
@article{3e7a9ab6-b949-4b65-97df-f650398a6e44,
  abstract     = {Background: The spectrum of human non-pylori Helicobacter infections is expanding, with species such as H. heilmannii and H. felis occasionally being associated with gastritis. However, the existence of non-pylori Helicobacter colonization in asymptomatic subjects has not been evaluated. The aim of this study was to investigate whether Helicobacter species other than pylori are present in the upper digestive tract of asymptomatic human subjects. Materials and methods: A Helicobacteraceae-specific semi-nested polymerase chain reaction (PCR) assay was used to detect Helicobacter-like organisms in the upper digestive tract of 91 Venezuelan volunteers (aged 18-68 years, 41 females, 50 males). Species were identified by denaturing gradient gel electrophoresis analysis and sequencing of the PCR products. Results: We detected DNA sharing 99-100% sequence identity in over 300-400 bp with the 16S rRNA genes of H. pylori, H. cetorum, and Candidatus Wolinella africanus in 76%, 16%, and 15% of the subjects, respectively. Multiple colonization was documented in 10% of the subjects: H. cetorum and Candidatus W. africanus (4%), H. pylori and Candidatus W. africanus (4%), and H. pylori and H. cetorum (2%). Conclusions: Our results suggest that non-pylori Helicobacteraceae colonization is relatively common in the Venezuelan asymptomatic population. This is the first report documenting the presence of H. cetorum DNA in the human upper digestive tract, and the second report of the recently discovered Candidatus W. africanus.},
  author       = {Garcia-Amado, M. Alexandra and Abu Al-Soud, Waleed and Borges-Landaez, Pedro and Contreras, Monica and Cedeno, Samandhy and Baez-Ramirez, Estalina and Dominguez-Bello, M. Gloria and Wadström, Torkel and Gueneau, Pulcherie},
  issn         = {1083-4389},
  keyword      = {PCR,Helicobacteraceae,prevalence},
  language     = {eng},
  number       = {5},
  pages        = {553--558},
  publisher    = {Wiley-Blackwell},
  series       = {Helicobacter},
  title        = {Non-pylori Helicobacteraceae in the upper digestive tract of asymptomatic venezuelan subjects: Detection of helicobacter cetorum-like and candidatus Wolinella africanus-like DNA},
  url          = {http://dx.doi.org/10.1111/j.1523-5378.2007.00526.x},
  volume       = {12},
  year         = {2007},
}