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Monoclonal antibody production using a new supermacroporous cryogel bioreactor

Nilsang, Suthasinee LU ; Nandakumar, Kutty Selva; Galaev, Igor LU ; Rakshit, Sudip Kumar; Hohndahl, Rikard; Mattiasson, Bo LU and Kumar, Ashok LU (2007) In Biotechnology Progress 23(4). p.932-939
Abstract
A supermacroporous cryogel bioreactor has been developed to culture hybridoma cells for long-term continuous production of monoclonal antibodies (mAb). Hybridoma clone M2139, secreting antibodies against J1 epitope (GERGAAGIAGPK; amino acids, 551-564) of collagen type II, are immobilized in the porous bed matrix of a cryogel column (10 mL bed volume). The cells got attached to the matrix within 48 h after inoculation and grew as a confluent sheet inside the cryogel matrix. Cells were in the lag phase for 15 days and secreted mAb into the circulation medium. Glucose consumption and lactic acid production were also monitored, and during the exponential phase (similar to 20 days), the hybridoma cell line consumed 0.75 mM day(-1) glucose,... (More)
A supermacroporous cryogel bioreactor has been developed to culture hybridoma cells for long-term continuous production of monoclonal antibodies (mAb). Hybridoma clone M2139, secreting antibodies against J1 epitope (GERGAAGIAGPK; amino acids, 551-564) of collagen type II, are immobilized in the porous bed matrix of a cryogel column (10 mL bed volume). The cells got attached to the matrix within 48 h after inoculation and grew as a confluent sheet inside the cryogel matrix. Cells were in the lag phase for 15 days and secreted mAb into the circulation medium. Glucose consumption and lactic acid production were also monitored, and during the exponential phase (similar to 20 days), the hybridoma cell line consumed 0.75 mM day(-1) glucose, produced 2.48 mM day(-1) lactic acid, and produced 6.5 mu g mL(-1) day(-1) mAb during the exponential phase. The mAb concentration reached 130 mu g mL(-1) after continuous run of the cryogel column for 36 days. The yield of the mAb after purification was 67.5 mg L-1, which was three times greater than the mAb yield obtained from T-flask batch cultivation. Even after the exchange of medium reservoir, cells in the cryogel column were still active and had relatively stable mAb production for an extended period of time. The bioreactor was operated continuously for 55 days without any contamination. The results from ELISA as well as arthritis experiments demonstrate that the antibodies secreted by cells grown on the cryogel column did not differ from antibodies purified from the cells grown in commercial CL-1000 culture flasks. Thus, supermacroporous cryogels can be useful as a supporting material for productive hybridoma cell culture. Cells were found to be viable inside the porous matrix of the cryogel during the study period and secreted antibodies continuously. The antibodies thus obtained from the cryogel reactor were found to be functionally active in vivo, as demonstrated by their capacity to induce arthritis in mice. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biotechnology Progress
volume
23
issue
4
pages
932 - 939
publisher
The American Chemical Society
external identifiers
  • wos:000248689400022
  • scopus:34547876521
ISSN
1520-6033
DOI
10.1021/bp0700399
language
English
LU publication?
yes
id
780ded7a-d9da-4dbd-9acc-4d06c730044a (old id 689623)
date added to LUP
2007-12-18 15:23:34
date last changed
2017-11-19 04:09:19
@article{780ded7a-d9da-4dbd-9acc-4d06c730044a,
  abstract     = {A supermacroporous cryogel bioreactor has been developed to culture hybridoma cells for long-term continuous production of monoclonal antibodies (mAb). Hybridoma clone M2139, secreting antibodies against J1 epitope (GERGAAGIAGPK; amino acids, 551-564) of collagen type II, are immobilized in the porous bed matrix of a cryogel column (10 mL bed volume). The cells got attached to the matrix within 48 h after inoculation and grew as a confluent sheet inside the cryogel matrix. Cells were in the lag phase for 15 days and secreted mAb into the circulation medium. Glucose consumption and lactic acid production were also monitored, and during the exponential phase (similar to 20 days), the hybridoma cell line consumed 0.75 mM day(-1) glucose, produced 2.48 mM day(-1) lactic acid, and produced 6.5 mu g mL(-1) day(-1) mAb during the exponential phase. The mAb concentration reached 130 mu g mL(-1) after continuous run of the cryogel column for 36 days. The yield of the mAb after purification was 67.5 mg L-1, which was three times greater than the mAb yield obtained from T-flask batch cultivation. Even after the exchange of medium reservoir, cells in the cryogel column were still active and had relatively stable mAb production for an extended period of time. The bioreactor was operated continuously for 55 days without any contamination. The results from ELISA as well as arthritis experiments demonstrate that the antibodies secreted by cells grown on the cryogel column did not differ from antibodies purified from the cells grown in commercial CL-1000 culture flasks. Thus, supermacroporous cryogels can be useful as a supporting material for productive hybridoma cell culture. Cells were found to be viable inside the porous matrix of the cryogel during the study period and secreted antibodies continuously. The antibodies thus obtained from the cryogel reactor were found to be functionally active in vivo, as demonstrated by their capacity to induce arthritis in mice.},
  author       = {Nilsang, Suthasinee and Nandakumar, Kutty Selva and Galaev, Igor and Rakshit, Sudip Kumar and Hohndahl, Rikard and Mattiasson, Bo and Kumar, Ashok},
  issn         = {1520-6033},
  language     = {eng},
  number       = {4},
  pages        = {932--939},
  publisher    = {The American Chemical Society},
  series       = {Biotechnology Progress},
  title        = {Monoclonal antibody production using a new supermacroporous cryogel bioreactor},
  url          = {http://dx.doi.org/10.1021/bp0700399},
  volume       = {23},
  year         = {2007},
}