Glyphosate-based herbicide induces long-lasting impairment in neuronal and glial differentiation
(2022) In Environmental Toxicology 37(8). p.2044-2057- Abstract
Glyphosate-based herbicides (GBH) are among the most sold pesticides in the world. There are several formulations based on the active ingredient glyphosate (GLY) used along with other chemicals to improve the absorption and penetration in plants. The final composition of commercial GBH may modify GLY toxicological profile, potentially enhancing its neurotoxic properties. The developing nervous system is particularly susceptible to insults occurring during the early phases of development, and exposure to chemicals in this period may lead to persistent impairments on neurogenesis and differentiation. The aim of this study was to evaluate the long-lasting effects of a sub-cytotoxic concentration, 2.5 parts per million of GBH and GLY, on... (More)
Glyphosate-based herbicides (GBH) are among the most sold pesticides in the world. There are several formulations based on the active ingredient glyphosate (GLY) used along with other chemicals to improve the absorption and penetration in plants. The final composition of commercial GBH may modify GLY toxicological profile, potentially enhancing its neurotoxic properties. The developing nervous system is particularly susceptible to insults occurring during the early phases of development, and exposure to chemicals in this period may lead to persistent impairments on neurogenesis and differentiation. The aim of this study was to evaluate the long-lasting effects of a sub-cytotoxic concentration, 2.5 parts per million of GBH and GLY, on the differentiation of human neuroepithelial stem cells (NES) derived from induced pluripotent stem cells (iPSC). We treated NES cells with each compound and evaluated the effects on key cellular processes, such as proliferation and differentiation in daughter cells never directly exposed to the toxicants. We found that GBH induced a more immature neuronal profile associated to increased PAX6, NESTIN and DCX expression, and a shift in the differentiation process toward glial cell fate at the expense of mature neurons, as shown by an increase in the glial markers GFAP, GLT1, GLAST and a decrease in MAP2. Such alterations were associated to dysregulation of key genes critically involved in neurogenesis, including PAX6, HES1, HES5, and DDK1. Altogether, the data indicate that subtoxic concentrations of GBH, but not of GLY, induce long-lasting impairments on the differentiation potential of NES cells.
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- author
- Reis, Luã ; Raciti, Marilena ; Rodriguez, Patricia González ; Joseph, Bertrand ; Al Rayyes, Ibrahim ; Uhlén, Per ; Falk, Anna LU ; da Cunha Lima, Suzana Telles and Ceccatelli, Sandra
- publishing date
- 2022-08
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Glycine/analogs & derivatives, Herbicides/toxicity, Humans, Neurogenesis, Neurons
- in
- Environmental Toxicology
- volume
- 37
- issue
- 8
- pages
- 2044 - 2057
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:85128986119
- pmid:35485992
- ISSN
- 1520-4081
- DOI
- 10.1002/tox.23549
- language
- English
- LU publication?
- no
- additional info
- © 2022 The Authors. Environmental Toxicology published by Wiley Periodicals LLC.
- id
- 68a33c34-c9f7-46a9-a6ed-da1bd5998b9f
- date added to LUP
- 2022-09-20 14:10:55
- date last changed
- 2024-06-15 01:00:00
@article{68a33c34-c9f7-46a9-a6ed-da1bd5998b9f,
abstract = {{<p>Glyphosate-based herbicides (GBH) are among the most sold pesticides in the world. There are several formulations based on the active ingredient glyphosate (GLY) used along with other chemicals to improve the absorption and penetration in plants. The final composition of commercial GBH may modify GLY toxicological profile, potentially enhancing its neurotoxic properties. The developing nervous system is particularly susceptible to insults occurring during the early phases of development, and exposure to chemicals in this period may lead to persistent impairments on neurogenesis and differentiation. The aim of this study was to evaluate the long-lasting effects of a sub-cytotoxic concentration, 2.5 parts per million of GBH and GLY, on the differentiation of human neuroepithelial stem cells (NES) derived from induced pluripotent stem cells (iPSC). We treated NES cells with each compound and evaluated the effects on key cellular processes, such as proliferation and differentiation in daughter cells never directly exposed to the toxicants. We found that GBH induced a more immature neuronal profile associated to increased PAX6, NESTIN and DCX expression, and a shift in the differentiation process toward glial cell fate at the expense of mature neurons, as shown by an increase in the glial markers GFAP, GLT1, GLAST and a decrease in MAP2. Such alterations were associated to dysregulation of key genes critically involved in neurogenesis, including PAX6, HES1, HES5, and DDK1. Altogether, the data indicate that subtoxic concentrations of GBH, but not of GLY, induce long-lasting impairments on the differentiation potential of NES cells.</p>}},
author = {{Reis, Luã and Raciti, Marilena and Rodriguez, Patricia González and Joseph, Bertrand and Al Rayyes, Ibrahim and Uhlén, Per and Falk, Anna and da Cunha Lima, Suzana Telles and Ceccatelli, Sandra}},
issn = {{1520-4081}},
keywords = {{Glycine/analogs & derivatives; Herbicides/toxicity; Humans; Neurogenesis; Neurons}},
language = {{eng}},
number = {{8}},
pages = {{2044--2057}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Environmental Toxicology}},
title = {{Glyphosate-based herbicide induces long-lasting impairment in neuronal and glial differentiation}},
url = {{http://dx.doi.org/10.1002/tox.23549}},
doi = {{10.1002/tox.23549}},
volume = {{37}},
year = {{2022}},
}