Isolated mouse islets as a model for studying insulin release
Lernmark, Åke LU
(1971)
In Acta Diabetologica Latina
8(1).
p.649-679
- Abstract
An in vitro system with microdissected mouse islets was employed for studying insulin release. Islets from obese-hyperglycemic mice were considered particularly useful in view of their high content of adequately functioning β-cells. After freeze-drying and weighing each of the incubated islets it was possible to express the rate of insulin release per islet dry weight. Insulin released from a single incubated islet could be measured radioimmunologically using crystalline mouse insulin as standard. The effects of various factors were examined, including diffusion of released insulin, islet size, shaking, temperature, pH, osmotic strength, oxygen tension, and treatment with enzymes. Provided that the basal rate was kept low, reproducible... (More)
An in vitro system with microdissected mouse islets was employed for studying insulin release. Islets from obese-hyperglycemic mice were considered particularly useful in view of their high content of adequately functioning β-cells. After freeze-drying and weighing each of the incubated islets it was possible to express the rate of insulin release per islet dry weight. Insulin released from a single incubated islet could be measured radioimmunologically using crystalline mouse insulin as standard. The effects of various factors were examined, including diffusion of released insulin, islet size, shaking, temperature, pH, osmotic strength, oxygen tension, and treatment with enzymes. Provided that the basal rate was kept low, reproducible values could be obtained for both the early and the late phases of the glucose-stimulated insulin release. Exposure of the microdissected islets to collagenase tended to increase the basal rate of insulin release but did not significantly affect the total insulin response to glucose nor the concomitant rise in the islet level of fructose-1,6-diphosphate. The present in vitro system offers a useful model for pharmacological screening on the insulin-releasing capacity of various compounds. It is also important that the rate of insulin release from the individual islet can be directly related to its metabolism.
(Less)
- author
- Lernmark, Åke
LU
- publishing date
- 1971-01-01
- type
- Contribution to journal
- publication status
- published
- keywords
- Collagenase, Glucose, Insulin release, Islet size, Mice, Microdissected pancreatic islets, Osmotic strength, Oxygen tension, Radioimmunoassay of mouse insulin, Temperature
- in
- Acta Diabetologica Latina
- volume
- 8
- issue
- 1
- pages
- 649 - 679
- publisher
- Springer
- external identifiers
-
- scopus:34250464536
- pmid:4942530
- ISSN
- 0001-5563
- DOI
- 10.1007/BF01550894
- language
- English
- LU publication?
- no
- id
- 68b15b98-2b35-4df9-94b5-323cbf3a431d
- date added to LUP
- 2019-09-18 12:25:08
- date last changed
- 2024-03-13 08:30:50
@article{68b15b98-2b35-4df9-94b5-323cbf3a431d,
abstract = {{<p>An in vitro system with microdissected mouse islets was employed for studying insulin release. Islets from obese-hyperglycemic mice were considered particularly useful in view of their high content of adequately functioning β-cells. After freeze-drying and weighing each of the incubated islets it was possible to express the rate of insulin release per islet dry weight. Insulin released from a single incubated islet could be measured radioimmunologically using crystalline mouse insulin as standard. The effects of various factors were examined, including diffusion of released insulin, islet size, shaking, temperature, pH, osmotic strength, oxygen tension, and treatment with enzymes. Provided that the basal rate was kept low, reproducible values could be obtained for both the early and the late phases of the glucose-stimulated insulin release. Exposure of the microdissected islets to collagenase tended to increase the basal rate of insulin release but did not significantly affect the total insulin response to glucose nor the concomitant rise in the islet level of fructose-1,6-diphosphate. The present in vitro system offers a useful model for pharmacological screening on the insulin-releasing capacity of various compounds. It is also important that the rate of insulin release from the individual islet can be directly related to its metabolism.</p>}},
author = {{Lernmark, Åke}},
issn = {{0001-5563}},
keywords = {{Collagenase; Glucose; Insulin release; Islet size; Mice; Microdissected pancreatic islets; Osmotic strength; Oxygen tension; Radioimmunoassay of mouse insulin; Temperature}},
language = {{eng}},
month = {{01}},
number = {{1}},
pages = {{649--679}},
publisher = {{Springer}},
series = {{Acta Diabetologica Latina}},
title = {{Isolated mouse islets as a model for studying insulin release}},
url = {{http://dx.doi.org/10.1007/BF01550894}},
doi = {{10.1007/BF01550894}},
volume = {{8}},
year = {{1971}},
}