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An ELISA for apolipoprotein M reveals a strong correlation to total cholesterol in human plasma

Axler, Olof LU ; Ahnström, Josefin LU and Dahlbäck, Björn LU (2007) In Journal of Lipid Research 48(8). p.1772-1780
Abstract
Apolipoprotein M (apoM) is a 188 amino acid, 25 kDa protein belonging to the lipocalin protein superfamily. Although predominantly associated with high density lipoprotein, apoM is found in all major lipoprotein classes. To facilitate clinical studies of apoM, we have developed a sandwich ELISA for the measurement of apoM in human plasma. This method has been used to investigate normal apoM variation and to establish reference values for healthy individuals through the measurement of 598 samples from the Nordic Reference Interval Project Bio-bank and Database ( NOBIDA) biobank. For women 18-49 years old, the reference interval for apoM was 0.58-1.18 mu mol/l, whereas for women 501 years and for men, the reference range was 0.61-1.30 mu... (More)
Apolipoprotein M (apoM) is a 188 amino acid, 25 kDa protein belonging to the lipocalin protein superfamily. Although predominantly associated with high density lipoprotein, apoM is found in all major lipoprotein classes. To facilitate clinical studies of apoM, we have developed a sandwich ELISA for the measurement of apoM in human plasma. This method has been used to investigate normal apoM variation and to establish reference values for healthy individuals through the measurement of 598 samples from the Nordic Reference Interval Project Bio-bank and Database ( NOBIDA) biobank. For women 18-49 years old, the reference interval for apoM was 0.58-1.18 mu mol/l, whereas for women 501 years and for men, the reference range was 0.61-1.30 mu mol/l. Correlation studies of apoM with 26 common clinical chemical analytes from the NOBIDA database revealed a marked positive correlation with plasma total cholesterol (r = 0.52) and LDL and HDL cholesterol ( r = 0.43 and 0.36, respectively). There was no statistically significant correlation with HDL/total cholesterol ratio or body mass index. In conclusion, a sandwich ELISA for the measurement of apoM in human plasma shows that apoM concentration is strongly correlated to total cholesterol in healthy individuals. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
enzyme-linked immunosorbent assay, interval project, lipocalin, nordic reference, and database, reference interval project bio-bank, nordic, biobank, reference range, reference interval, high density lipoprotein, low density lipoprotein
in
Journal of Lipid Research
volume
48
issue
8
pages
1772 - 1780
publisher
American Society for Biochemistry and Molecular Biology
external identifiers
  • wos:000248044200012
  • scopus:34548176847
ISSN
1539-7262
DOI
10.1194/jlr.M700113-JLR200
language
English
LU publication?
yes
id
56f33d01-60ff-428d-8336-680273c041b8 (old id 691677)
date added to LUP
2007-12-07 11:46:27
date last changed
2017-11-05 03:41:21
@article{56f33d01-60ff-428d-8336-680273c041b8,
  abstract     = {Apolipoprotein M (apoM) is a 188 amino acid, 25 kDa protein belonging to the lipocalin protein superfamily. Although predominantly associated with high density lipoprotein, apoM is found in all major lipoprotein classes. To facilitate clinical studies of apoM, we have developed a sandwich ELISA for the measurement of apoM in human plasma. This method has been used to investigate normal apoM variation and to establish reference values for healthy individuals through the measurement of 598 samples from the Nordic Reference Interval Project Bio-bank and Database ( NOBIDA) biobank. For women 18-49 years old, the reference interval for apoM was 0.58-1.18 mu mol/l, whereas for women 501 years and for men, the reference range was 0.61-1.30 mu mol/l. Correlation studies of apoM with 26 common clinical chemical analytes from the NOBIDA database revealed a marked positive correlation with plasma total cholesterol (r = 0.52) and LDL and HDL cholesterol ( r = 0.43 and 0.36, respectively). There was no statistically significant correlation with HDL/total cholesterol ratio or body mass index. In conclusion, a sandwich ELISA for the measurement of apoM in human plasma shows that apoM concentration is strongly correlated to total cholesterol in healthy individuals.},
  author       = {Axler, Olof and Ahnström, Josefin and Dahlbäck, Björn},
  issn         = {1539-7262},
  keyword      = {enzyme-linked immunosorbent assay,interval project,lipocalin,nordic reference,and database,reference interval project bio-bank,nordic,biobank,reference range,reference interval,high density lipoprotein,low density lipoprotein},
  language     = {eng},
  number       = {8},
  pages        = {1772--1780},
  publisher    = {American Society for Biochemistry and Molecular Biology},
  series       = {Journal of Lipid Research},
  title        = {An ELISA for apolipoprotein M reveals a strong correlation to total cholesterol in human plasma},
  url          = {http://dx.doi.org/10.1194/jlr.M700113-JLR200},
  volume       = {48},
  year         = {2007},
}