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Amperometric monitoring of redox activity in intact, permeabilised and lyophilised cells of the yeast Hansenula polymorpha

Khupova, Maria; Kuznetsov, Boris; Gonchar, Mykhailo; Ruzgas, Tautgrdas and Shleev, Sergey LU (2007) In Electrochemistry Communications 9(7). p.1480-1485
Abstract
An effect of permeabilisation and lyophilisation of the yeast cells Hansenula polymorpha on their electrochemical behaviour in the presence of mediators, substrates (formaldehyde, glucose, methanol, ethanol), and cofactors (NAD(+), NADP(+), NADH, NADPH, glutathione) has been studied. Two amperometric techniques differing in the cell immobilisation methods were applied. The cells of a wild strain (356) and mutant strains (C-105 and KCA 33) of the yeast, grown in the presence of glucose or methanol, were used in the experiments. The intact cells revealed the highest reduction rates of mediators, 2,6-dichlorphenolindophenol (DCIP) and 2,4-benzoquinone (BQ) as measured by amperometry. The addition of formaldehyde significantly enhanced the... (More)
An effect of permeabilisation and lyophilisation of the yeast cells Hansenula polymorpha on their electrochemical behaviour in the presence of mediators, substrates (formaldehyde, glucose, methanol, ethanol), and cofactors (NAD(+), NADP(+), NADH, NADPH, glutathione) has been studied. Two amperometric techniques differing in the cell immobilisation methods were applied. The cells of a wild strain (356) and mutant strains (C-105 and KCA 33) of the yeast, grown in the presence of glucose or methanol, were used in the experiments. The intact cells revealed the highest reduction rates of mediators, 2,6-dichlorphenolindophenol (DCIP) and 2,4-benzoquinone (BQ) as measured by amperometry. The addition of formaldehyde significantly enhanced the response, if the cells were grown in the presence of glucose. The permeabilised cells showed the lowest current level in the presence of DCIP and BQ and no response to the addition of formaldehyde and NAD(+). However, the addition of NADH gave significant current surge. All these phenomena imply that the permeabilised cells lost cofactors and the activity of dehydrogenases producing NADH, but they remained the activity of NADH-ubiquinone oxidoreductase and of some components of the electron transport chain. The electrochemical behaviour of the lyophilised cells shows they are heterogeneous. The partial degradation of the outer membrane of the cells after their lyophilisation was electrochemically confirmed. (c) 2007 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
mediators, amperometry, cells, permeabilised and lyophilised, yeast Hansenula polymorpha, intact
in
Electrochemistry Communications
volume
9
issue
7
pages
1480 - 1485
publisher
Elsevier
external identifiers
  • wos:000248089400011
  • scopus:34250184971
ISSN
1388-2481
DOI
10.1016/j.elecom.2007.02.011
language
English
LU publication?
yes
id
d690d7ae-5850-4773-8a43-0ff9974e9c55 (old id 692691)
date added to LUP
2007-12-13 16:38:03
date last changed
2017-01-01 04:26:29
@article{d690d7ae-5850-4773-8a43-0ff9974e9c55,
  abstract     = {An effect of permeabilisation and lyophilisation of the yeast cells Hansenula polymorpha on their electrochemical behaviour in the presence of mediators, substrates (formaldehyde, glucose, methanol, ethanol), and cofactors (NAD(+), NADP(+), NADH, NADPH, glutathione) has been studied. Two amperometric techniques differing in the cell immobilisation methods were applied. The cells of a wild strain (356) and mutant strains (C-105 and KCA 33) of the yeast, grown in the presence of glucose or methanol, were used in the experiments. The intact cells revealed the highest reduction rates of mediators, 2,6-dichlorphenolindophenol (DCIP) and 2,4-benzoquinone (BQ) as measured by amperometry. The addition of formaldehyde significantly enhanced the response, if the cells were grown in the presence of glucose. The permeabilised cells showed the lowest current level in the presence of DCIP and BQ and no response to the addition of formaldehyde and NAD(+). However, the addition of NADH gave significant current surge. All these phenomena imply that the permeabilised cells lost cofactors and the activity of dehydrogenases producing NADH, but they remained the activity of NADH-ubiquinone oxidoreductase and of some components of the electron transport chain. The electrochemical behaviour of the lyophilised cells shows they are heterogeneous. The partial degradation of the outer membrane of the cells after their lyophilisation was electrochemically confirmed. (c) 2007 Elsevier B.V. All rights reserved.},
  author       = {Khupova, Maria and Kuznetsov, Boris and Gonchar, Mykhailo and Ruzgas, Tautgrdas and Shleev, Sergey},
  issn         = {1388-2481},
  keyword      = {mediators,amperometry,cells,permeabilised and lyophilised,yeast Hansenula polymorpha,intact},
  language     = {eng},
  number       = {7},
  pages        = {1480--1485},
  publisher    = {Elsevier},
  series       = {Electrochemistry Communications},
  title        = {Amperometric monitoring of redox activity in intact, permeabilised and lyophilised cells of the yeast Hansenula polymorpha},
  url          = {http://dx.doi.org/10.1016/j.elecom.2007.02.011},
  volume       = {9},
  year         = {2007},
}