Development of a microbial biosensor based on carbon nanotube (CNT) modified electrodes
(2007) In Electrochemistry Communications 9(7). p.1810-1815- Abstract
- Pseudomonas putida DSM 50026 cells were used as the biological component and the measurement was based on the respiratory activity of the cells estimated from electrochemical measurements. The cells were immobilised on carbon nanotube (CNT) modified carbon paste electrodes (CPE) by means of a redox osmium polymer, viz. poly(1-vinylimidazole)(12)-[Os-(4,4'-dimethyl-2,2'-dipyridyl)(2)Cl-2](2+ /+). The osmium polymer efficiently shuttles electrons between redox enzymes located in the cell wall of the cells and promotes a stable binding to the electrode surface. The effect of varying the amounts of CNT and osmium polymer on the response to glucose was investigated to find the optimum composition of the sensor. The effects of pH and temperature... (More)
- Pseudomonas putida DSM 50026 cells were used as the biological component and the measurement was based on the respiratory activity of the cells estimated from electrochemical measurements. The cells were immobilised on carbon nanotube (CNT) modified carbon paste electrodes (CPE) by means of a redox osmium polymer, viz. poly(1-vinylimidazole)(12)-[Os-(4,4'-dimethyl-2,2'-dipyridyl)(2)Cl-2](2+ /+). The osmium polymer efficiently shuttles electrons between redox enzymes located in the cell wall of the cells and promotes a stable binding to the electrode surface. The effect of varying the amounts of CNT and osmium polymer on the response to glucose was investigated to find the optimum composition of the sensor. The effects of pH and temperature were also examined. After the optimisation studies, the system was characterised by using glucose as substrate. Moreover, the microbial biosensor was also prepared by using phenol adapted bacteria and then, calibrated to phenol. After that, it was applied for phenol detection in an artificial waste water sample. (C) 2007 Published by Elsevier B.V. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/692697
- author
- Timur, Suna ; Anik, Ulku ; Odaci, Dilek and Gorton, Lo LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Pseudomonas, carbon nanotube (CNT), biosensor, osmium redox polymer, putida
- in
- Electrochemistry Communications
- volume
- 9
- issue
- 7
- pages
- 1810 - 1815
- publisher
- Elsevier
- external identifiers
-
- wos:000248089400073
- scopus:34250353544
- ISSN
- 1388-2481
- DOI
- 10.1016/j.elecom.2007.04.012
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- d9a92516-2892-448a-a359-bd4dc4cf2b95 (old id 692697)
- date added to LUP
- 2016-04-01 12:07:54
- date last changed
- 2022-04-21 02:53:12
@article{d9a92516-2892-448a-a359-bd4dc4cf2b95, abstract = {{Pseudomonas putida DSM 50026 cells were used as the biological component and the measurement was based on the respiratory activity of the cells estimated from electrochemical measurements. The cells were immobilised on carbon nanotube (CNT) modified carbon paste electrodes (CPE) by means of a redox osmium polymer, viz. poly(1-vinylimidazole)(12)-[Os-(4,4'-dimethyl-2,2'-dipyridyl)(2)Cl-2](2+ /+). The osmium polymer efficiently shuttles electrons between redox enzymes located in the cell wall of the cells and promotes a stable binding to the electrode surface. The effect of varying the amounts of CNT and osmium polymer on the response to glucose was investigated to find the optimum composition of the sensor. The effects of pH and temperature were also examined. After the optimisation studies, the system was characterised by using glucose as substrate. Moreover, the microbial biosensor was also prepared by using phenol adapted bacteria and then, calibrated to phenol. After that, it was applied for phenol detection in an artificial waste water sample. (C) 2007 Published by Elsevier B.V.}}, author = {{Timur, Suna and Anik, Ulku and Odaci, Dilek and Gorton, Lo}}, issn = {{1388-2481}}, keywords = {{Pseudomonas; carbon nanotube (CNT); biosensor; osmium redox polymer; putida}}, language = {{eng}}, number = {{7}}, pages = {{1810--1815}}, publisher = {{Elsevier}}, series = {{Electrochemistry Communications}}, title = {{Development of a microbial biosensor based on carbon nanotube (CNT) modified electrodes}}, url = {{http://dx.doi.org/10.1016/j.elecom.2007.04.012}}, doi = {{10.1016/j.elecom.2007.04.012}}, volume = {{9}}, year = {{2007}}, }