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Development of a microbial biosensor based on carbon nanotube (CNT) modified electrodes

Timur, Suna; Anik, Ulku; Odaci, Dilek and Gorton, Lo LU (2007) In Electrochemistry Communications 9(7). p.1810-1815
Abstract
Pseudomonas putida DSM 50026 cells were used as the biological component and the measurement was based on the respiratory activity of the cells estimated from electrochemical measurements. The cells were immobilised on carbon nanotube (CNT) modified carbon paste electrodes (CPE) by means of a redox osmium polymer, viz. poly(1-vinylimidazole)(12)-[Os-(4,4'-dimethyl-2,2'-dipyridyl)(2)Cl-2](2+ /+). The osmium polymer efficiently shuttles electrons between redox enzymes located in the cell wall of the cells and promotes a stable binding to the electrode surface. The effect of varying the amounts of CNT and osmium polymer on the response to glucose was investigated to find the optimum composition of the sensor. The effects of pH and temperature... (More)
Pseudomonas putida DSM 50026 cells were used as the biological component and the measurement was based on the respiratory activity of the cells estimated from electrochemical measurements. The cells were immobilised on carbon nanotube (CNT) modified carbon paste electrodes (CPE) by means of a redox osmium polymer, viz. poly(1-vinylimidazole)(12)-[Os-(4,4'-dimethyl-2,2'-dipyridyl)(2)Cl-2](2+ /+). The osmium polymer efficiently shuttles electrons between redox enzymes located in the cell wall of the cells and promotes a stable binding to the electrode surface. The effect of varying the amounts of CNT and osmium polymer on the response to glucose was investigated to find the optimum composition of the sensor. The effects of pH and temperature were also examined. After the optimisation studies, the system was characterised by using glucose as substrate. Moreover, the microbial biosensor was also prepared by using phenol adapted bacteria and then, calibrated to phenol. After that, it was applied for phenol detection in an artificial waste water sample. (C) 2007 Published by Elsevier B.V. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Pseudomonas, carbon nanotube (CNT), biosensor, osmium redox polymer, putida
in
Electrochemistry Communications
volume
9
issue
7
pages
1810 - 1815
publisher
Elsevier
external identifiers
  • wos:000248089400073
  • scopus:34250353544
ISSN
1388-2481
DOI
10.1016/j.elecom.2007.04.012
language
English
LU publication?
yes
id
d9a92516-2892-448a-a359-bd4dc4cf2b95 (old id 692697)
date added to LUP
2008-01-03 10:45:38
date last changed
2017-09-10 03:42:06
@article{d9a92516-2892-448a-a359-bd4dc4cf2b95,
  abstract     = {Pseudomonas putida DSM 50026 cells were used as the biological component and the measurement was based on the respiratory activity of the cells estimated from electrochemical measurements. The cells were immobilised on carbon nanotube (CNT) modified carbon paste electrodes (CPE) by means of a redox osmium polymer, viz. poly(1-vinylimidazole)(12)-[Os-(4,4'-dimethyl-2,2'-dipyridyl)(2)Cl-2](2+ /+). The osmium polymer efficiently shuttles electrons between redox enzymes located in the cell wall of the cells and promotes a stable binding to the electrode surface. The effect of varying the amounts of CNT and osmium polymer on the response to glucose was investigated to find the optimum composition of the sensor. The effects of pH and temperature were also examined. After the optimisation studies, the system was characterised by using glucose as substrate. Moreover, the microbial biosensor was also prepared by using phenol adapted bacteria and then, calibrated to phenol. After that, it was applied for phenol detection in an artificial waste water sample. (C) 2007 Published by Elsevier B.V.},
  author       = {Timur, Suna and Anik, Ulku and Odaci, Dilek and Gorton, Lo},
  issn         = {1388-2481},
  keyword      = {Pseudomonas,carbon nanotube (CNT),biosensor,osmium redox polymer,putida},
  language     = {eng},
  number       = {7},
  pages        = {1810--1815},
  publisher    = {Elsevier},
  series       = {Electrochemistry Communications},
  title        = {Development of a microbial biosensor based on carbon nanotube (CNT) modified electrodes},
  url          = {http://dx.doi.org/10.1016/j.elecom.2007.04.012},
  volume       = {9},
  year         = {2007},
}