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The collagen structure of the tympanic membrane - Collagen types I, II, and III in the healthy tympanic membrane, during healing of a perforation, and during infection

Stenfeldt, Karin LU orcid ; Johansson, C and Hellstrom, S (2006) In Archives of Otolaryngology - Head and Neck Surgery 132(3). p.293-298
Abstract
Objective: To analyze the distribution of 3 collagen types in healthy tympanic membranes, during healing of a perforation, and during infection. Design: Immunohistochemical study of collagen types I, II, and III in the tympanic membranes of healthy rats as well as during healing of a perforation and in the presence of infection with Streptococcus pneumoniae at various time points. Setting: Laboratory research center at a university hospital. Results: Type II collagen was a main constituent of the lamina propria of the pars tensa, whereas type I collagen was found mainly in the pars flaccida. Collagen types I and III were found at the insertion to the malleus handle and in the loose connective tissue surrounding the main collagen layer of... (More)
Objective: To analyze the distribution of 3 collagen types in healthy tympanic membranes, during healing of a perforation, and during infection. Design: Immunohistochemical study of collagen types I, II, and III in the tympanic membranes of healthy rats as well as during healing of a perforation and in the presence of infection with Streptococcus pneumoniae at various time points. Setting: Laboratory research center at a university hospital. Results: Type II collagen was a main constituent of the lamina propria of the pars tensa, whereas type I collagen was found mainly in the pars flaccida. Collagen types I and III were found at the insertion to the malleus handle and in the loose connective tissue surrounding the main collagen layer of the pars tensa. After myringotomy, collagen types I and III were found at the perforation border and around dilated blood vessels early in the healing phase. During infection, the collagen layer was thickened and stained strongly for type II collagen. Collagen types I and III were found in the edematous connective tissue around the main collagen layer and around dilated blood vessels. Three months after perforation or infection, all 3 collagens were present in the lamina propria of the tympanic membrane. Extensive amounts of all 3 collagen types were present in the scar tissue in the tympanic membranes of rats that had undergone myringotomy during the presence of acute otitis media. Conclusions: The lamina propria of the pars tensa is mainly made up of type II collagen, whereas type I collagen is found in the pars flaccida. Thus the fibrous structure of the pars tensa and flaccida is composed of diverse collagen types, which reflects the different physiological properties of these tissues. Collagen types I and III are present in the acute healing phase after myringotomy and infection, and the collagen content of the tympanic membrane is modified during the inflammatory and healing process. (Less)
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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Archives of Otolaryngology - Head and Neck Surgery
volume
132
issue
3
pages
293 - 298
publisher
American Medical Association
external identifiers
  • wos:000236120100008
  • pmid:16549750
  • scopus:33645242679
ISSN
1538-361X
language
English
LU publication?
yes
id
b42746b4-90c4-4ff3-88a4-9e181404218e (old id 693500)
alternative location
http://archotol.ama-assn.org/cgi/content/full/132/3/293
date added to LUP
2016-04-01 12:04:55
date last changed
2020-11-03 03:05:31
@article{b42746b4-90c4-4ff3-88a4-9e181404218e,
  abstract     = {Objective: To analyze the distribution of 3 collagen types in healthy tympanic membranes, during healing of a perforation, and during infection. Design: Immunohistochemical study of collagen types I, II, and III in the tympanic membranes of healthy rats as well as during healing of a perforation and in the presence of infection with Streptococcus pneumoniae at various time points. Setting: Laboratory research center at a university hospital. Results: Type II collagen was a main constituent of the lamina propria of the pars tensa, whereas type I collagen was found mainly in the pars flaccida. Collagen types I and III were found at the insertion to the malleus handle and in the loose connective tissue surrounding the main collagen layer of the pars tensa. After myringotomy, collagen types I and III were found at the perforation border and around dilated blood vessels early in the healing phase. During infection, the collagen layer was thickened and stained strongly for type II collagen. Collagen types I and III were found in the edematous connective tissue around the main collagen layer and around dilated blood vessels. Three months after perforation or infection, all 3 collagens were present in the lamina propria of the tympanic membrane. Extensive amounts of all 3 collagen types were present in the scar tissue in the tympanic membranes of rats that had undergone myringotomy during the presence of acute otitis media. Conclusions: The lamina propria of the pars tensa is mainly made up of type II collagen, whereas type I collagen is found in the pars flaccida. Thus the fibrous structure of the pars tensa and flaccida is composed of diverse collagen types, which reflects the different physiological properties of these tissues. Collagen types I and III are present in the acute healing phase after myringotomy and infection, and the collagen content of the tympanic membrane is modified during the inflammatory and healing process.},
  author       = {Stenfeldt, Karin and Johansson, C and Hellstrom, S},
  issn         = {1538-361X},
  language     = {eng},
  number       = {3},
  pages        = {293--298},
  publisher    = {American Medical Association},
  series       = {Archives of Otolaryngology - Head and Neck Surgery},
  title        = {The collagen structure of the tympanic membrane - Collagen types I, II, and III in the healthy tympanic membrane, during healing of a perforation, and during infection},
  url          = {http://archotol.ama-assn.org/cgi/content/full/132/3/293},
  volume       = {132},
  year         = {2006},
}