Epidemiological MIC cut-off values for tigecycline calculated from Etest MIC values using normalized resistance interpretation
(2006) In Journal of Antimicrobial Chemotherapy 57(3). p.498-505- Abstract
- Objectives: To apply the normalized resistance interpretation (NRI) method to Etest MIC results which have higher precision than conventional log(2) dilution MIC tests due to the inclusion of intermediate values. If successful, NRI might provide an objective tool for the definition of epidemiological MIC cut-off values. Methods: MICs of tigecycline and other antimicrobial agents were determined for 4771 clinical isolates comprising five Gram-positive and 13 Gram-negative species or species groups using the Etest. Histograms of MIC values were constructed for each species and NRI calculations were applied to them. An upper MIC limit of 2.5 SD above the theoretical mean of the normalized distribution was used for setting the epidemiological... (More)
- Objectives: To apply the normalized resistance interpretation (NRI) method to Etest MIC results which have higher precision than conventional log(2) dilution MIC tests due to the inclusion of intermediate values. If successful, NRI might provide an objective tool for the definition of epidemiological MIC cut-off values. Methods: MICs of tigecycline and other antimicrobial agents were determined for 4771 clinical isolates comprising five Gram-positive and 13 Gram-negative species or species groups using the Etest. Histograms of MIC values were constructed for each species and NRI calculations were applied to them. An upper MIC limit of 2.5 SD above the theoretical mean of the normalized distribution was used for setting the epidemiological cut-off values. Results: Calculated cut-off values for wild-type strains were between 0.11 and 0.96 mg/L for Gram-positive species, and between 0.44 and 8.3 mg/L for Gram-negative species, except for Pseudomonas aeruginosa, which had a cut-off value of 450 mg/L, consistent with earlier reports on the lack of activity of tigecycline against this species. Conclusions: NRI offers an objective method for the analysis of MICs produced using Etests and the determination of epidemiological MIC cut-off values. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/693624
- author
- Kronvall, G ; Karlsson, I ; Walder, Mats LU ; Sorberg, M and Nilsson, LE
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- drug resistance, wild-type MIC distributions, clinical microbiology, antimicrobial susceptibility tests, MIC breakpoints
- in
- Journal of Antimicrobial Chemotherapy
- volume
- 57
- issue
- 3
- pages
- 498 - 505
- publisher
- Oxford University Press
- external identifiers
-
- wos:000235282500014
- pmid:16410264
- scopus:32644438347
- ISSN
- 1460-2091
- DOI
- 10.1093/jac/dki489
- language
- English
- LU publication?
- yes
- id
- 8ccadaaf-b6f6-40af-940a-065dc9416dec (old id 693624)
- date added to LUP
- 2016-04-01 12:30:23
- date last changed
- 2022-04-21 08:21:35
@article{8ccadaaf-b6f6-40af-940a-065dc9416dec,
abstract = {{Objectives: To apply the normalized resistance interpretation (NRI) method to Etest MIC results which have higher precision than conventional log(2) dilution MIC tests due to the inclusion of intermediate values. If successful, NRI might provide an objective tool for the definition of epidemiological MIC cut-off values. Methods: MICs of tigecycline and other antimicrobial agents were determined for 4771 clinical isolates comprising five Gram-positive and 13 Gram-negative species or species groups using the Etest. Histograms of MIC values were constructed for each species and NRI calculations were applied to them. An upper MIC limit of 2.5 SD above the theoretical mean of the normalized distribution was used for setting the epidemiological cut-off values. Results: Calculated cut-off values for wild-type strains were between 0.11 and 0.96 mg/L for Gram-positive species, and between 0.44 and 8.3 mg/L for Gram-negative species, except for Pseudomonas aeruginosa, which had a cut-off value of 450 mg/L, consistent with earlier reports on the lack of activity of tigecycline against this species. Conclusions: NRI offers an objective method for the analysis of MICs produced using Etests and the determination of epidemiological MIC cut-off values.}},
author = {{Kronvall, G and Karlsson, I and Walder, Mats and Sorberg, M and Nilsson, LE}},
issn = {{1460-2091}},
keywords = {{drug resistance; wild-type MIC distributions; clinical microbiology; antimicrobial susceptibility tests; MIC breakpoints}},
language = {{eng}},
number = {{3}},
pages = {{498--505}},
publisher = {{Oxford University Press}},
series = {{Journal of Antimicrobial Chemotherapy}},
title = {{Epidemiological MIC cut-off values for tigecycline calculated from Etest MIC values using normalized resistance interpretation}},
url = {{http://dx.doi.org/10.1093/jac/dki489}},
doi = {{10.1093/jac/dki489}},
volume = {{57}},
year = {{2006}},
}