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Stimulation and inhibition of insulin release by an amino-reactive probe of plasma membrane

Hellman, Bo ; Idahl, Lars Åke ; Lernmark, Åke LU orcid ; Sehlin, Janove and Täljedal, Inge Bert (1973) In Journal of Membrane Biology 14(1). p.135-142
Abstract

4-Acetamido-4′-isothiocyanostilbene-2,2′-disulphonic acid (SITS), an amino-reacting probe of plasma membranes, stimulated the release of insulin from micro-dissected pancreatic islets of ob/ob-mice. This effect of SITS was inhibited by adrenaline or by calcium deficiency. SITS did not inhibit the insulin-releasing action of glucose or leucine but rather potentiated the effect of glucose. In contrast, SITS markedly depressed the insulin secretory response to chloromercuribenzene-p-sulphonic acid. It is suggested that by reacting with the plasma membranes SITS may induce secretagogic ionic fluxes in the β-cells. In addition, SITS apparently inhibits the secretagogic recognition of chloromercuribenzene-p-sulphonic acid, presumably by... (More)

4-Acetamido-4′-isothiocyanostilbene-2,2′-disulphonic acid (SITS), an amino-reacting probe of plasma membranes, stimulated the release of insulin from micro-dissected pancreatic islets of ob/ob-mice. This effect of SITS was inhibited by adrenaline or by calcium deficiency. SITS did not inhibit the insulin-releasing action of glucose or leucine but rather potentiated the effect of glucose. In contrast, SITS markedly depressed the insulin secretory response to chloromercuribenzene-p-sulphonic acid. It is suggested that by reacting with the plasma membranes SITS may induce secretagogic ionic fluxes in the β-cells. In addition, SITS apparently inhibits the secretagogic recognition of chloromercuribenzene-p-sulphonic acid, presumably by preventing the organic mercurial from reacting with certain membrane thiol groups.

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author
; ; ; and
publishing date
type
Contribution to journal
publication status
published
in
Journal of Membrane Biology
volume
14
issue
1
pages
135 - 142
publisher
Springer
external identifiers
  • scopus:0015748472
  • pmid:4590247
ISSN
0022-2631
DOI
10.1007/BF01868074
language
English
LU publication?
no
id
6950c68a-4702-4aef-92a2-5ee5cc917570
date added to LUP
2019-09-18 12:21:17
date last changed
2024-03-13 08:01:08
@article{6950c68a-4702-4aef-92a2-5ee5cc917570,
  abstract     = {{<p>4-Acetamido-4′-isothiocyanostilbene-2,2′-disulphonic acid (SITS), an amino-reacting probe of plasma membranes, stimulated the release of insulin from micro-dissected pancreatic islets of ob/ob-mice. This effect of SITS was inhibited by adrenaline or by calcium deficiency. SITS did not inhibit the insulin-releasing action of glucose or leucine but rather potentiated the effect of glucose. In contrast, SITS markedly depressed the insulin secretory response to chloromercuribenzene-p-sulphonic acid. It is suggested that by reacting with the plasma membranes SITS may induce secretagogic ionic fluxes in the β-cells. In addition, SITS apparently inhibits the secretagogic recognition of chloromercuribenzene-p-sulphonic acid, presumably by preventing the organic mercurial from reacting with certain membrane thiol groups.</p>}},
  author       = {{Hellman, Bo and Idahl, Lars Åke and Lernmark, Åke and Sehlin, Janove and Täljedal, Inge Bert}},
  issn         = {{0022-2631}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{1}},
  pages        = {{135--142}},
  publisher    = {{Springer}},
  series       = {{Journal of Membrane Biology}},
  title        = {{Stimulation and inhibition of insulin release by an amino-reactive probe of plasma membrane}},
  url          = {{http://dx.doi.org/10.1007/BF01868074}},
  doi          = {{10.1007/BF01868074}},
  volume       = {{14}},
  year         = {{1973}},
}