Stimulation and inhibition of insulin release by an amino-reactive probe of plasma membrane
(1973) In Journal of Membrane Biology 14(1). p.135-142- Abstract
4-Acetamido-4′-isothiocyanostilbene-2,2′-disulphonic acid (SITS), an amino-reacting probe of plasma membranes, stimulated the release of insulin from micro-dissected pancreatic islets of ob/ob-mice. This effect of SITS was inhibited by adrenaline or by calcium deficiency. SITS did not inhibit the insulin-releasing action of glucose or leucine but rather potentiated the effect of glucose. In contrast, SITS markedly depressed the insulin secretory response to chloromercuribenzene-p-sulphonic acid. It is suggested that by reacting with the plasma membranes SITS may induce secretagogic ionic fluxes in the β-cells. In addition, SITS apparently inhibits the secretagogic recognition of chloromercuribenzene-p-sulphonic acid, presumably by... (More)
4-Acetamido-4′-isothiocyanostilbene-2,2′-disulphonic acid (SITS), an amino-reacting probe of plasma membranes, stimulated the release of insulin from micro-dissected pancreatic islets of ob/ob-mice. This effect of SITS was inhibited by adrenaline or by calcium deficiency. SITS did not inhibit the insulin-releasing action of glucose or leucine but rather potentiated the effect of glucose. In contrast, SITS markedly depressed the insulin secretory response to chloromercuribenzene-p-sulphonic acid. It is suggested that by reacting with the plasma membranes SITS may induce secretagogic ionic fluxes in the β-cells. In addition, SITS apparently inhibits the secretagogic recognition of chloromercuribenzene-p-sulphonic acid, presumably by preventing the organic mercurial from reacting with certain membrane thiol groups.
(Less)
- author
- Hellman, Bo ; Idahl, Lars Åke ; Lernmark, Åke LU ; Sehlin, Janove and Täljedal, Inge Bert
- publishing date
- 1973-12-01
- type
- Contribution to journal
- publication status
- published
- in
- Journal of Membrane Biology
- volume
- 14
- issue
- 1
- pages
- 135 - 142
- publisher
- Springer
- external identifiers
-
- pmid:4590247
- scopus:0015748472
- ISSN
- 0022-2631
- DOI
- 10.1007/BF01868074
- language
- English
- LU publication?
- no
- id
- 6950c68a-4702-4aef-92a2-5ee5cc917570
- date added to LUP
- 2019-09-18 12:21:17
- date last changed
- 2024-03-13 08:01:08
@article{6950c68a-4702-4aef-92a2-5ee5cc917570, abstract = {{<p>4-Acetamido-4′-isothiocyanostilbene-2,2′-disulphonic acid (SITS), an amino-reacting probe of plasma membranes, stimulated the release of insulin from micro-dissected pancreatic islets of ob/ob-mice. This effect of SITS was inhibited by adrenaline or by calcium deficiency. SITS did not inhibit the insulin-releasing action of glucose or leucine but rather potentiated the effect of glucose. In contrast, SITS markedly depressed the insulin secretory response to chloromercuribenzene-p-sulphonic acid. It is suggested that by reacting with the plasma membranes SITS may induce secretagogic ionic fluxes in the β-cells. In addition, SITS apparently inhibits the secretagogic recognition of chloromercuribenzene-p-sulphonic acid, presumably by preventing the organic mercurial from reacting with certain membrane thiol groups.</p>}}, author = {{Hellman, Bo and Idahl, Lars Åke and Lernmark, Åke and Sehlin, Janove and Täljedal, Inge Bert}}, issn = {{0022-2631}}, language = {{eng}}, month = {{12}}, number = {{1}}, pages = {{135--142}}, publisher = {{Springer}}, series = {{Journal of Membrane Biology}}, title = {{Stimulation and inhibition of insulin release by an amino-reactive probe of plasma membrane}}, url = {{http://dx.doi.org/10.1007/BF01868074}}, doi = {{10.1007/BF01868074}}, volume = {{14}}, year = {{1973}}, }