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Peptide Derivatized Lamellar Aggregates as Target-Specific MRI Contrast Agents

Tesauro, Diego; Accardo, Antonella; Gianolio, Eliana; Luigi, Paduano; Teixera, Jos; Schillén, Karin LU ; Aime, Silvio and Morelli, Giancarlo (2007) In ChemBioChem 8(8). p.950-955
Abstract
The relaxivity behaviour and the structural characterization of new supramolecular aggregates (bilayer structures and micelles) obtained by combining two different amphiphilic monomers are reported. One monomer, (C18)2DTPAGlu-Gd, contains a very stable gadolinium complex, and the other, DSPE-PEG2000-CCK8, contains the bioactive CCK8 peptide. Samples that contained only DSPE-PEG2000-CCK8, or up to 50 % (C18)2DTPAGlu-Gd, aggregated as double-layer structures (lamellar aggregates) with a thickness of80-100 Å, as evaluated by SANS measurement and Cryo-TEM imaging. A transition to micelle formation was observed when the amount of (C18)2DTPAGlu-Gd in the aggregate was increased. These were rod-like micelles 40 Å in radius and >200 Å in... (More)
The relaxivity behaviour and the structural characterization of new supramolecular aggregates (bilayer structures and micelles) obtained by combining two different amphiphilic monomers are reported. One monomer, (C18)2DTPAGlu-Gd, contains a very stable gadolinium complex, and the other, DSPE-PEG2000-CCK8, contains the bioactive CCK8 peptide. Samples that contained only DSPE-PEG2000-CCK8, or up to 50 % (C18)2DTPAGlu-Gd, aggregated as double-layer structures (lamellar aggregates) with a thickness of80-100 Å, as evaluated by SANS measurement and Cryo-TEM imaging. A transition to micelle formation was observed when the amount of (C18)2DTPAGlu-Gd in the aggregate was increased. These were rod-like micelles 40 Å in radius and >200 Å in length. The proton relaxivities for both lamellar aggregates and rod-like micelles were the same (17.2 mM-1 s-1), although the values were the results of different combinations of local and global contributions. The in vitro target selectivity of aggregates that contained the CCK-8 peptide was demonstrated by using nuclear medicine techniques. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
complexes, imaging agents, SANS, peptides, lamellar aggregates
in
ChemBioChem
volume
8
issue
8
pages
950 - 955
publisher
John Wiley & Sons
external identifiers
  • wos:000246981000016
  • scopus:34447512737
ISSN
1439-4227
DOI
10.1002/cbic.200700077
language
English
LU publication?
yes
id
035b2bf3-3799-4994-98de-fc59c5f515da (old id 697177)
date added to LUP
2007-12-19 14:22:48
date last changed
2017-03-05 03:39:11
@article{035b2bf3-3799-4994-98de-fc59c5f515da,
  abstract     = {The relaxivity behaviour and the structural characterization of new supramolecular aggregates (bilayer structures and micelles) obtained by combining two different amphiphilic monomers are reported. One monomer, (C18)2DTPAGlu-Gd, contains a very stable gadolinium complex, and the other, DSPE-PEG2000-CCK8, contains the bioactive CCK8 peptide. Samples that contained only DSPE-PEG2000-CCK8, or up to 50 % (C18)2DTPAGlu-Gd, aggregated as double-layer structures (lamellar aggregates) with a thickness of80-100 Å, as evaluated by SANS measurement and Cryo-TEM imaging. A transition to micelle formation was observed when the amount of (C18)2DTPAGlu-Gd in the aggregate was increased. These were rod-like micelles 40 Å in radius and >200 Å in length. The proton relaxivities for both lamellar aggregates and rod-like micelles were the same (17.2 mM-1 s-1), although the values were the results of different combinations of local and global contributions. The in vitro target selectivity of aggregates that contained the CCK-8 peptide was demonstrated by using nuclear medicine techniques.},
  author       = {Tesauro, Diego and Accardo, Antonella and Gianolio, Eliana and Luigi, Paduano and Teixera, Jos and Schillén, Karin and Aime, Silvio and Morelli, Giancarlo},
  issn         = {1439-4227},
  keyword      = {complexes,imaging agents,SANS,peptides,lamellar aggregates},
  language     = {eng},
  number       = {8},
  pages        = {950--955},
  publisher    = {John Wiley & Sons},
  series       = {ChemBioChem},
  title        = {Peptide Derivatized Lamellar Aggregates as Target-Specific MRI Contrast Agents},
  url          = {http://dx.doi.org/10.1002/cbic.200700077},
  volume       = {8},
  year         = {2007},
}