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On the Enzymatic Activation of NADH

Meijers, Rob ; Morris, Richard J ; Adolph, Hans W ; Merli, Angelo ; Lamzin, Victor S and Cedergren, Eila LU (2001) In Journal of Biological Chemistry 276(12). p.9316-9321
Abstract
Atomic (1 Å) resolution x-ray structures of horse liver alcohol dehydrogenase in complex with NADH revealed the formation of an adduct in the active site between a metal-bound water and NADH. Furthermore, a pronounced distortion of the pyridine ring of NADH was observed. A series of quantum chemical calculations on the water-nicotinamide adduct showed that the puckering of the pyridine ring in the crystal structures can only be reproduced when the water is considered a hydroxide ion. These observations provide fundamental insight into the enzymatic activation of NADH for hydride transfer.
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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Biological Chemistry
volume
276
issue
12
pages
9316 - 9321
publisher
American Society for Biochemistry and Molecular Biology
external identifiers
  • scopus:0035937753
  • pmid:11134046
ISSN
1083-351X
DOI
10.1074/jbc.M010870200
language
English
LU publication?
yes
id
69733d3b-43a8-41b9-96c2-b16ec8d829b4 (old id 125030)
date added to LUP
2016-04-01 12:38:44
date last changed
2025-10-14 11:18:25
@article{69733d3b-43a8-41b9-96c2-b16ec8d829b4,
  abstract     = {{Atomic (1 Å) resolution x-ray structures of horse liver alcohol dehydrogenase in complex with NADH revealed the formation of an adduct in the active site between a metal-bound water and NADH. Furthermore, a pronounced distortion of the pyridine ring of NADH was observed. A series of quantum chemical calculations on the water-nicotinamide adduct showed that the puckering of the pyridine ring in the crystal structures can only be reproduced when the water is considered a hydroxide ion. These observations provide fundamental insight into the enzymatic activation of NADH for hydride transfer.}},
  author       = {{Meijers, Rob and Morris, Richard J and Adolph, Hans W and Merli, Angelo and Lamzin, Victor S and Cedergren, Eila}},
  issn         = {{1083-351X}},
  language     = {{eng}},
  number       = {{12}},
  pages        = {{9316--9321}},
  publisher    = {{American Society for Biochemistry and Molecular Biology}},
  series       = {{Journal of Biological Chemistry}},
  title        = {{On the Enzymatic Activation of NADH}},
  url          = {{http://dx.doi.org/10.1074/jbc.M010870200}},
  doi          = {{10.1074/jbc.M010870200}},
  volume       = {{276}},
  year         = {{2001}},
}