A large-scale purification of paclitaxel from cell cultures of Taxus chinensis
(2004) In Process Biochemistry 39(12). p.1985-1991- Abstract
- A large-scale purification method was developed for producing paclitaxel, to guarantee high purity and yield from plant cell cultures. The complete method for mass production was a simple and efficient procedure, for the isolation and purification of paclitaxel from the biomass of Taxus chinensis, consisting of solvent extraction, synthetic adsorbent treatment, and two steps of precipitation, followed by two steps of high performance liquid chromatography (HPLC). The organic solvent extraction of biomass obtained crude extract containing paclitaxel. The use of synthetic adsorbent treatment and precipitation in the prepurification process allows for rapid and efficient separation of paclitaxel from interfering compounds and dramatically... (More)
- A large-scale purification method was developed for producing paclitaxel, to guarantee high purity and yield from plant cell cultures. The complete method for mass production was a simple and efficient procedure, for the isolation and purification of paclitaxel from the biomass of Taxus chinensis, consisting of solvent extraction, synthetic adsorbent treatment, and two steps of precipitation, followed by two steps of high performance liquid chromatography (HPLC). The organic solvent extraction of biomass obtained crude extract containing paclitaxel. The use of synthetic adsorbent treatment and precipitation in the prepurification process allows for rapid and efficient separation of paclitaxel from interfering compounds and dramatically increases the yield and purity of crude paclitaxel for HPLC purification steps compared to alternative processes. This prepurification process serves to minimise solvent usage, size, and complexity of the HPLC operations for paclitaxel purification. The paclitaxel of over 99.5% purity can be simply obtained with high yield from crude paclitaxel by HPLC using reverse-phase separation on C18 as the first step and normal-phase separation on silica as the second step. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/69acb6df-efe6-4374-b10b-18990661871a
- author
- Pyo, Sang-Hyun LU ; Park, Heung-Bok ; Song, Bong-Kyu and Han, Byung-Hee
- publishing date
- 2004-10-29
- type
- Contribution to journal
- publication status
- published
- keywords
- Plant cell culture, Paclitaxel
- in
- Process Biochemistry
- volume
- 39
- issue
- 12
- pages
- 1985 - 1991
- publisher
- Elsevier
- external identifiers
-
- scopus:4344631816
- ISSN
- 1359-5113
- DOI
- 10.1016/j.procbio.2003.09.028
- language
- English
- LU publication?
- no
- id
- 69acb6df-efe6-4374-b10b-18990661871a
- date added to LUP
- 2024-12-26 16:19:46
- date last changed
- 2025-01-16 12:44:02
@article{69acb6df-efe6-4374-b10b-18990661871a,
abstract = {{A large-scale purification method was developed for producing paclitaxel, to guarantee high purity and yield from plant cell cultures. The complete method for mass production was a simple and efficient procedure, for the isolation and purification of paclitaxel from the biomass of Taxus chinensis, consisting of solvent extraction, synthetic adsorbent treatment, and two steps of precipitation, followed by two steps of high performance liquid chromatography (HPLC). The organic solvent extraction of biomass obtained crude extract containing paclitaxel. The use of synthetic adsorbent treatment and precipitation in the prepurification process allows for rapid and efficient separation of paclitaxel from interfering compounds and dramatically increases the yield and purity of crude paclitaxel for HPLC purification steps compared to alternative processes. This prepurification process serves to minimise solvent usage, size, and complexity of the HPLC operations for paclitaxel purification. The paclitaxel of over 99.5% purity can be simply obtained with high yield from crude paclitaxel by HPLC using reverse-phase separation on C18 as the first step and normal-phase separation on silica as the second step.}},
author = {{Pyo, Sang-Hyun and Park, Heung-Bok and Song, Bong-Kyu and Han, Byung-Hee}},
issn = {{1359-5113}},
keywords = {{Plant cell culture; Paclitaxel}},
language = {{eng}},
month = {{10}},
number = {{12}},
pages = {{1985--1991}},
publisher = {{Elsevier}},
series = {{Process Biochemistry}},
title = {{A large-scale purification of paclitaxel from cell cultures of <i>Taxus chinensis</i>}},
url = {{http://dx.doi.org/10.1016/j.procbio.2003.09.028}},
doi = {{10.1016/j.procbio.2003.09.028}},
volume = {{39}},
year = {{2004}},
}