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Reprogramming cell fates : Insights from combinatorial approaches

Pereira, Carlos-Filipe LU ; Lemischka, Ihor R. and Moore, Kateri (2012) In Annals of the New York Academy of Sciences 1266(1). p.7-17
Abstract

Epigenetic reprogramming can be achieved in different ways, including nuclear transfer, cell fusion, or the expression of transcription factors (TFs). Combinatorial overexpression provides an opportunity to define the minimal core network of TFs that instructs specific cell fates. This approach has been employed to induce mouse and human pluripotency and differentiated cell types from cells that can be also as distant as cells from different germ layers. This suggests the possibility that any specific cell type may be directly converted into another if the appropriate reprogramming TF core is determined. Herein, we review the factors used for reprogramming multiple cell identities and raise the question of whether there is a common... (More)

Epigenetic reprogramming can be achieved in different ways, including nuclear transfer, cell fusion, or the expression of transcription factors (TFs). Combinatorial overexpression provides an opportunity to define the minimal core network of TFs that instructs specific cell fates. This approach has been employed to induce mouse and human pluripotency and differentiated cell types from cells that can be also as distant as cells from different germ layers. This suggests the possibility that any specific cell type may be directly converted into another if the appropriate reprogramming TF core is determined. Herein, we review the factors used for reprogramming multiple cell identities and raise the question of whether there is a common underlying blueprint for reprogramming factors. In addition to the generation of human cell types of interest for cell-replacement therapies, we propose that the TF-mediated conversion of differentiated cell types, especially somatic stem cells, will have an impact on our understanding of their biological development.

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Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
keywords
Direct conversion, Induced pluripotency, IPS, Reprogramming, Transcription factor, Transdetermination
in
Annals of the New York Academy of Sciences
volume
1266
issue
1
pages
11 pages
publisher
New York Academy of Sciences
external identifiers
  • scopus:84865351152
ISSN
0077-8923
DOI
10.1111/j.1749-6632.2012.06508.x
language
English
LU publication?
no
id
69b16d33-edae-45bb-b362-526ed0f3fede
date added to LUP
2017-10-02 17:27:16
date last changed
2017-10-13 09:21:16
@article{69b16d33-edae-45bb-b362-526ed0f3fede,
  abstract     = {<p>Epigenetic reprogramming can be achieved in different ways, including nuclear transfer, cell fusion, or the expression of transcription factors (TFs). Combinatorial overexpression provides an opportunity to define the minimal core network of TFs that instructs specific cell fates. This approach has been employed to induce mouse and human pluripotency and differentiated cell types from cells that can be also as distant as cells from different germ layers. This suggests the possibility that any specific cell type may be directly converted into another if the appropriate reprogramming TF core is determined. Herein, we review the factors used for reprogramming multiple cell identities and raise the question of whether there is a common underlying blueprint for reprogramming factors. In addition to the generation of human cell types of interest for cell-replacement therapies, we propose that the TF-mediated conversion of differentiated cell types, especially somatic stem cells, will have an impact on our understanding of their biological development.</p>},
  author       = {Pereira, Carlos-Filipe and Lemischka, Ihor R. and Moore, Kateri},
  issn         = {0077-8923},
  keyword      = {Direct conversion,Induced pluripotency,IPS,Reprogramming,Transcription factor,Transdetermination},
  language     = {eng},
  number       = {1},
  pages        = {7--17},
  publisher    = {New York Academy of Sciences},
  series       = {Annals of the New York Academy of Sciences},
  title        = {Reprogramming cell fates : Insights from combinatorial approaches},
  url          = {http://dx.doi.org/10.1111/j.1749-6632.2012.06508.x},
  volume       = {1266},
  year         = {2012},
}