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Cyclosporin A does not Inhibit IL‐lα‐Induced Epithelial Cell IL‐6 Secretion

HEDGES, S. ; AGACE, W. LU ; SVENSSON, M. and SVANBORG, C. LU (1993) In Scandinavian Journal of Immunology 37(5). p.581-586
Abstract

Trauma and infection activated a murine mucosal IL‐6 response in different ways: the IL‐6 response to bacteria was sensitive to Cyclosporin A (CsA); the IL‐6 response to trauma was not. The aim of the present study was to identify possible activators of the CsA‐insensitive IL‐6 secretion at the epithelial cell level. Two human epithelial cell lines from the kidney (A498) and bladder (J82) were exposed to Escherichia coli Hu734, interleukin‐lα (IL‐lα) and tumour necrosis factor a (TNF‐α). The E. coli strain had been used for the in vivo experiments which led to this study, and IL‐lα and TNF‐α were likely to be released during infections and trauma. The secretion of IL‐6 into the supernatants was compared between cells stimulated in the... (More)

Trauma and infection activated a murine mucosal IL‐6 response in different ways: the IL‐6 response to bacteria was sensitive to Cyclosporin A (CsA); the IL‐6 response to trauma was not. The aim of the present study was to identify possible activators of the CsA‐insensitive IL‐6 secretion at the epithelial cell level. Two human epithelial cell lines from the kidney (A498) and bladder (J82) were exposed to Escherichia coli Hu734, interleukin‐lα (IL‐lα) and tumour necrosis factor a (TNF‐α). The E. coli strain had been used for the in vivo experiments which led to this study, and IL‐lα and TNF‐α were likely to be released during infections and trauma. The secretion of IL‐6 into the supernatants was compared between cells stimulated in the presence or absence of CsA. E. coli Hu734, IL‐lα and TNF‐α stimulated an IL‐6 response in the two epithelial cell lines. The IL‐lα‐induced IL‐6 response was rapid, and the secreted IL‐6 levels were significantly higher than those induced by E. coli Hu734 or TNF‐α. The IL‐6 response to IL‐ lα was insensitive to CsA. By contrast, the IL‐6 response to E. coli Hu734 and TNF‐α was inhibited by CsA. These results demonstrated that the inhibitory effect of CsA depends on the stimulus triggering the IL‐6 response. IL‐lα may play a role in the induction of trauma‐associated CsA‐insensitive IL‐6 secretion.

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published
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Scandinavian Journal of Immunology
volume
37
issue
5
pages
6 pages
publisher
Wiley-Blackwell
external identifiers
  • pmid:8484104
  • scopus:0027155178
ISSN
0300-9475
DOI
10.1111/j.1365-3083.1993.tb02575.x
language
English
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yes
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69c09fa7-ac3c-409e-bf78-da82c3c233cd
date added to LUP
2019-05-30 14:03:49
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2020-01-13 01:56:35
@article{69c09fa7-ac3c-409e-bf78-da82c3c233cd,
  abstract     = {<p>Trauma and infection activated a murine mucosal IL‐6 response in different ways: the IL‐6 response to bacteria was sensitive to Cyclosporin A (CsA); the IL‐6 response to trauma was not. The aim of the present study was to identify possible activators of the CsA‐insensitive IL‐6 secretion at the epithelial cell level. Two human epithelial cell lines from the kidney (A498) and bladder (J82) were exposed to Escherichia coli Hu734, interleukin‐lα (IL‐lα) and tumour necrosis factor a (TNF‐α). The E. coli strain had been used for the in vivo experiments which led to this study, and IL‐lα and TNF‐α were likely to be released during infections and trauma. The secretion of IL‐6 into the supernatants was compared between cells stimulated in the presence or absence of CsA. E. coli Hu734, IL‐lα and TNF‐α stimulated an IL‐6 response in the two epithelial cell lines. The IL‐lα‐induced IL‐6 response was rapid, and the secreted IL‐6 levels were significantly higher than those induced by E. coli Hu734 or TNF‐α. The IL‐6 response to IL‐ lα was insensitive to CsA. By contrast, the IL‐6 response to E. coli Hu734 and TNF‐α was inhibited by CsA. These results demonstrated that the inhibitory effect of CsA depends on the stimulus triggering the IL‐6 response. IL‐lα may play a role in the induction of trauma‐associated CsA‐insensitive IL‐6 secretion.</p>},
  author       = {HEDGES, S. and AGACE, W. and SVENSSON, M. and SVANBORG, C.},
  issn         = {0300-9475},
  language     = {eng},
  month        = {01},
  number       = {5},
  pages        = {581--586},
  publisher    = {Wiley-Blackwell},
  series       = {Scandinavian Journal of Immunology},
  title        = {Cyclosporin A does not Inhibit IL‐lα‐Induced Epithelial Cell IL‐6 Secretion},
  url          = {http://dx.doi.org/10.1111/j.1365-3083.1993.tb02575.x},
  doi          = {10.1111/j.1365-3083.1993.tb02575.x},
  volume       = {37},
  year         = {1993},
}