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The isothiocyanate produced from glucomoringin inhibits NF-kB and reduces myeloma growth in nude mice in vivo

Brunelli, Dario; Tavecchio, Michele LU ; Falcioni, Cristiano; Frapolli, Roberta; Erba, Eugenio; Iori, Renato; Rollin, Patrick; Barillari, Jessica; Manzotti, Carla and Morazzoni, Paolo, et al. (2010) In Biochemical Pharmacology 79(8). p.8-1141
Abstract

Glucosinolates (GLs), natural compounds extracted from Brassicaceae and precursors of isothiocyanates (ITCs), have been studied in the last decades mostly due to their chemopreventive activity and, more recently, for their potential use as novel chemotherapeutics. The aim of the present study was to investigate the in vitro and in vivo activity of glucomoringin (GMG), an uncommon member of the GLs family, and to compare it with glucoraphanin (GRA), one of the most studied GL. We have evaluated the potency of both compounds in inducing cell death, cell cycle perturbations, apoptosis, NF-kB inhibition and GST-pi activity in human carcinoma cells with different GST-pi contents as well as in human multiple myeloma and leukaemia cell lines.... (More)

Glucosinolates (GLs), natural compounds extracted from Brassicaceae and precursors of isothiocyanates (ITCs), have been studied in the last decades mostly due to their chemopreventive activity and, more recently, for their potential use as novel chemotherapeutics. The aim of the present study was to investigate the in vitro and in vivo activity of glucomoringin (GMG), an uncommon member of the GLs family, and to compare it with glucoraphanin (GRA), one of the most studied GL. We have evaluated the potency of both compounds in inducing cell death, cell cycle perturbations, apoptosis, NF-kB inhibition and GST-pi activity in human carcinoma cells with different GST-pi contents as well as in human multiple myeloma and leukaemia cell lines. GMG-derived ITC (GMG-ITC) showed to be more effective compared to GRA-derived ITC (Sulforaphane), especially in inhibiting NF-kB activity and inducing apoptosis through a caspase-dependent pathway; these effects were more pronounced in myeloma cells, in which we could also observe a long lasting growth inhibitory effect, probably due to NF-kB inhibition, which is considered essential for myeloma cell survival. Both GLs were able to induce cell death in the muM range in all tested cell lines but caused cell cycle perturbations only in myeloma cells; they were also able to modulate the GST/GSH pathway by causing a 3-fold increase in GST-pi activity in MCF7 cells. In vivo study showed that pure GMG-ITC was only slightly active in a carcinoma mice model, whereas it had significant antitumoral activity in a myeloma model, causing little toxicity.

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keywords
Animals, Cell Cycle, Cell Line, Tumor, Cell Proliferation, Female, Glucosinolates, Glutathione S-Transferase pi, Glutathione Transferase, Humans, Imidoesters, Isothiocyanates, Mice, Mice, Nude, Multiple Myeloma, NF-kappa B, Journal Article, Research Support, Non-U.S. Gov't
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Biochemical Pharmacology
volume
79
issue
8
pages
8 pages
publisher
Elsevier
external identifiers
  • scopus:77249125260
ISSN
0006-2952
DOI
10.1016/j.bcp.2009.12.008
language
English
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no
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69d34603-ea4a-4cc4-87f5-2ed9c2c09be9
date added to LUP
2017-03-07 09:12:24
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2018-07-01 04:43:50
@article{69d34603-ea4a-4cc4-87f5-2ed9c2c09be9,
  abstract     = {<p>Glucosinolates (GLs), natural compounds extracted from Brassicaceae and precursors of isothiocyanates (ITCs), have been studied in the last decades mostly due to their chemopreventive activity and, more recently, for their potential use as novel chemotherapeutics. The aim of the present study was to investigate the in vitro and in vivo activity of glucomoringin (GMG), an uncommon member of the GLs family, and to compare it with glucoraphanin (GRA), one of the most studied GL. We have evaluated the potency of both compounds in inducing cell death, cell cycle perturbations, apoptosis, NF-kB inhibition and GST-pi activity in human carcinoma cells with different GST-pi contents as well as in human multiple myeloma and leukaemia cell lines. GMG-derived ITC (GMG-ITC) showed to be more effective compared to GRA-derived ITC (Sulforaphane), especially in inhibiting NF-kB activity and inducing apoptosis through a caspase-dependent pathway; these effects were more pronounced in myeloma cells, in which we could also observe a long lasting growth inhibitory effect, probably due to NF-kB inhibition, which is considered essential for myeloma cell survival. Both GLs were able to induce cell death in the muM range in all tested cell lines but caused cell cycle perturbations only in myeloma cells; they were also able to modulate the GST/GSH pathway by causing a 3-fold increase in GST-pi activity in MCF7 cells. In vivo study showed that pure GMG-ITC was only slightly active in a carcinoma mice model, whereas it had significant antitumoral activity in a myeloma model, causing little toxicity.</p>},
  author       = {Brunelli, Dario and Tavecchio, Michele and Falcioni, Cristiano and Frapolli, Roberta and Erba, Eugenio and Iori, Renato and Rollin, Patrick and Barillari, Jessica and Manzotti, Carla and Morazzoni, Paolo and D'Incalci, Maurizio},
  issn         = {0006-2952},
  keyword      = {Animals,Cell Cycle,Cell Line, Tumor,Cell Proliferation,Female,Glucosinolates,Glutathione S-Transferase pi,Glutathione Transferase,Humans,Imidoesters,Isothiocyanates,Mice,Mice, Nude,Multiple Myeloma,NF-kappa B,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  month        = {04},
  number       = {8},
  pages        = {8--1141},
  publisher    = {Elsevier},
  series       = {Biochemical Pharmacology},
  title        = {The isothiocyanate produced from glucomoringin inhibits NF-kB and reduces myeloma growth in nude mice in vivo},
  url          = {http://dx.doi.org/10.1016/j.bcp.2009.12.008},
  volume       = {79},
  year         = {2010},
}