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Mineralization of cortical bone during maturation and growth in rabbits

Törnquist, Elin LU ; Isaksson, Hanna LU and Turunen, Mikael J. LU (2019) In Journal of Bone and Mineral Metabolism
Abstract

Introduction: The composite nature of bone as a material governs its structure and mechanical behavior. How the collagenous matrix mineralizes, in terms of both mineral deposition and structure of the mineral crystals, is highly interesting when trying to elucidate the complex structural changes that occur during bone growth and maturation. We have previously looked at mineral deposition and structural evolution of the collagenous matrix, linking both to changes in mechanics. The purpose of this study was to provide specific information on changes in crystal size and organization as a function of growth and maturation. 

Materials and Methods: Using micro-computed tomography (µCT) and micro-focused scanning small-angle X-ray... (More)

Introduction: The composite nature of bone as a material governs its structure and mechanical behavior. How the collagenous matrix mineralizes, in terms of both mineral deposition and structure of the mineral crystals, is highly interesting when trying to elucidate the complex structural changes that occur during bone growth and maturation. We have previously looked at mineral deposition and structural evolution of the collagenous matrix, linking both to changes in mechanics. The purpose of this study was to provide specific information on changes in crystal size and organization as a function of growth and maturation. 

Materials and Methods: Using micro-computed tomography (µCT) and micro-focused scanning small-angle X-ray scattering (SAXS) we investigated cortical bone in two orthogonal directions relative to the long axis of the humeri of New Zealand White rabbits spanning from new-born to 6-months of age. We also investigated the changes with tissue age by looking at radial profiles of osteonal structures in the 6-months old rabbits. The findings were compared to our previous compositional, structural and mechanical data on the same sample cohort. 

Results: µCT showed a continuous mineral deposition up until 3-months of age, whilst the SAXS data showed an increase in both crystal thickness and degree of orientation up until 6-months of age. The osteonal profiles showed no statistically significant changes in crystal thickness. 

Conclusions: Comparison to previously collected mechanical data suggests that changes are not only explained by amount of mineral in the tissue but also by the crystal dimensions.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bone, Micro-CT, Mineral crystal thickness, Osteon, Small-angle X-ray scattering
in
Journal of Bone and Mineral Metabolism
publisher
Springer
external identifiers
  • scopus:85075989536
  • pmid:31807903
ISSN
0914-8779
DOI
10.1007/s00774-019-01068-y
language
English
LU publication?
yes
id
6a1024ca-36b7-4a7a-a745-448eb44eb67f
date added to LUP
2020-01-02 14:37:25
date last changed
2020-01-03 03:11:56
@article{6a1024ca-36b7-4a7a-a745-448eb44eb67f,
  abstract     = {<p>Introduction: The composite nature of bone as a material governs its structure and mechanical behavior. How the collagenous matrix mineralizes, in terms of both mineral deposition and structure of the mineral crystals, is highly interesting when trying to elucidate the complex structural changes that occur during bone growth and maturation. We have previously looked at mineral deposition and structural evolution of the collagenous matrix, linking both to changes in mechanics. The purpose of this study was to provide specific information on changes in crystal size and organization as a function of growth and maturation. </p><p>Materials and Methods: Using micro-computed tomography (µCT) and micro-focused scanning small-angle X-ray scattering (SAXS) we investigated cortical bone in two orthogonal directions relative to the long axis of the humeri of New Zealand White rabbits spanning from new-born to 6-months of age. We also investigated the changes with tissue age by looking at radial profiles of osteonal structures in the 6-months old rabbits. The findings were compared to our previous compositional, structural and mechanical data on the same sample cohort. </p><p>Results: µCT showed a continuous mineral deposition up until 3-months of age, whilst the SAXS data showed an increase in both crystal thickness and degree of orientation up until 6-months of age. The osteonal profiles showed no statistically significant changes in crystal thickness. </p><p>Conclusions: Comparison to previously collected mechanical data suggests that changes are not only explained by amount of mineral in the tissue but also by the crystal dimensions.</p>},
  author       = {Törnquist, Elin and Isaksson, Hanna and Turunen, Mikael J.},
  issn         = {0914-8779},
  language     = {eng},
  month        = {12},
  publisher    = {Springer},
  series       = {Journal of Bone and Mineral Metabolism},
  title        = {Mineralization of cortical bone during maturation and growth in rabbits},
  url          = {http://dx.doi.org/10.1007/s00774-019-01068-y},
  doi          = {10.1007/s00774-019-01068-y},
  year         = {2019},
}