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Method for lipidomic analysis : p53 expression modulation of sulfatide, ganglioside, and phospholipid composition of U87 MG glioblastoma cells

He, Huan; Conrad, Charles A.; Nilsson, Carol L LU ; Ji, Yongjie; Schaub, Tanner M; Marshall, Alan G and Emmett, Mark R (2007) In Analytical Chemistry 79(22). p.30-8423
Abstract

Lipidomics can complement genomics and proteomics by providing new insight into dynamic changes in biomembranes; however, few reports in the literature have explored, on an organism-wide scale, the functional link between nonenzymatic proteins and cellular lipids. Here, we report changes induced by adenovirus-delivered wild-type p53 gene and chemotherapy of U87 MG glioblastoma cells, a treatment known to trigger apoptosis and cell cycle arrest. We compare polar lipid changes in treated cells and control cells by use of a novel, sensitive method that employs lipid extraction, one-step liquid chromatography separation, high-resolution mass analysis, and Kendrick mass defect analysis. Nano-LC FT-ICR MS and quadrupole linear ion trap MS/MS... (More)

Lipidomics can complement genomics and proteomics by providing new insight into dynamic changes in biomembranes; however, few reports in the literature have explored, on an organism-wide scale, the functional link between nonenzymatic proteins and cellular lipids. Here, we report changes induced by adenovirus-delivered wild-type p53 gene and chemotherapy of U87 MG glioblastoma cells, a treatment known to trigger apoptosis and cell cycle arrest. We compare polar lipid changes in treated cells and control cells by use of a novel, sensitive method that employs lipid extraction, one-step liquid chromatography separation, high-resolution mass analysis, and Kendrick mass defect analysis. Nano-LC FT-ICR MS and quadrupole linear ion trap MS/MS analysis of polar lipids yields hundreds of unique assignments of glyco- and phospholipids at sub-ppm mass accuracy and high resolving power (m/Deltam50% = 200 000 at m/z 400) at 1 s/scan. MS/MS data confirm molecular structures in many instances. Sulfatides are most highly modulated by wild-type p53 treatment. The treatment also leads to an increase in phospholipids such as phosphatidyl inositols, phosphatidyl serines, phosphatidyl glycerols, and phosphatidyl ethanolamines. An increase in hydroxylated phospholipids is especially noteworthy. Also, a decrease in the longer chain gangliosides, GD1 and GM1b, is observed in wild-type p53 (treated) cells.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Cell Line, Tumor, Chromatography, Liquid, Gangliosides, Glioblastoma, Humans, Molecular Structure, Phospholipids, Spectrometry, Mass, Electrospray Ionization, Spectroscopy, Fourier Transform Infrared, Sulfoglycosphingolipids, Tumor Suppressor Protein p53, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.
in
Analytical Chemistry
volume
79
issue
22
pages
8 pages
publisher
The American Chemical Society
external identifiers
  • scopus:36448939111
ISSN
0003-2700
DOI
10.1021/ac071413m
language
English
LU publication?
no
id
6a166b5d-827e-4286-8624-95cb7e5de4c5
date added to LUP
2017-05-16 10:32:22
date last changed
2017-07-30 05:25:12
@article{6a166b5d-827e-4286-8624-95cb7e5de4c5,
  abstract     = {<p>Lipidomics can complement genomics and proteomics by providing new insight into dynamic changes in biomembranes; however, few reports in the literature have explored, on an organism-wide scale, the functional link between nonenzymatic proteins and cellular lipids. Here, we report changes induced by adenovirus-delivered wild-type p53 gene and chemotherapy of U87 MG glioblastoma cells, a treatment known to trigger apoptosis and cell cycle arrest. We compare polar lipid changes in treated cells and control cells by use of a novel, sensitive method that employs lipid extraction, one-step liquid chromatography separation, high-resolution mass analysis, and Kendrick mass defect analysis. Nano-LC FT-ICR MS and quadrupole linear ion trap MS/MS analysis of polar lipids yields hundreds of unique assignments of glyco- and phospholipids at sub-ppm mass accuracy and high resolving power (m/Deltam50% = 200 000 at m/z 400) at 1 s/scan. MS/MS data confirm molecular structures in many instances. Sulfatides are most highly modulated by wild-type p53 treatment. The treatment also leads to an increase in phospholipids such as phosphatidyl inositols, phosphatidyl serines, phosphatidyl glycerols, and phosphatidyl ethanolamines. An increase in hydroxylated phospholipids is especially noteworthy. Also, a decrease in the longer chain gangliosides, GD1 and GM1b, is observed in wild-type p53 (treated) cells.</p>},
  author       = {He, Huan and Conrad, Charles A. and Nilsson, Carol L and Ji, Yongjie and Schaub, Tanner M and Marshall, Alan G and Emmett, Mark R},
  issn         = {0003-2700},
  keyword      = {Cell Line, Tumor,Chromatography, Liquid,Gangliosides,Glioblastoma,Humans,Molecular Structure,Phospholipids,Spectrometry, Mass, Electrospray Ionization,Spectroscopy, Fourier Transform Infrared,Sulfoglycosphingolipids,Tumor Suppressor Protein p53,Journal Article,Research Support, Non-U.S. Gov't,Research Support, U.S. Gov't, Non-P.H.S.},
  language     = {eng},
  month        = {11},
  number       = {22},
  pages        = {30--8423},
  publisher    = {The American Chemical Society},
  series       = {Analytical Chemistry},
  title        = {Method for lipidomic analysis : p53 expression modulation of sulfatide, ganglioside, and phospholipid composition of U87 MG glioblastoma cells},
  url          = {http://dx.doi.org/10.1021/ac071413m},
  volume       = {79},
  year         = {2007},
}