Expression and Regulation of a Novel Decidual Cells-Derived Estrogen Target during Decidualization
Lu, Lin ; Chen, Yingni ; Yang, Zhenshan LU
; Liang, Shijin
; Zhu, Songqi
and Liang, Xiaohuan
(2023)
In International Journal of Molecular Sciences
24(1).
- Abstract
During decidualization in rodents, uterine stromal cells undergo extensive reprogramming to differentiate into distinct cell types, forming primary decidual zones (PDZs), secondary decidual zones (SDZs), and layers of undifferentiated stromal cells. The formation of secondary decidual zones is accompanied by extensive angiogenesis. During early pregnancy, besides ovarian estrogen, de novo synthesis of estrogen in the uterus is essential for the progress of decidualization. However, the molecular mechanisms are not fully understood. Studies have shown that Cystatin B (Cstb) is highly expressed in the decidual tissue of the uterus, but the regulation and mechanism of Cstb in the process of decidualization have not been reported. Our... (More)
During decidualization in rodents, uterine stromal cells undergo extensive reprogramming to differentiate into distinct cell types, forming primary decidual zones (PDZs), secondary decidual zones (SDZs), and layers of undifferentiated stromal cells. The formation of secondary decidual zones is accompanied by extensive angiogenesis. During early pregnancy, besides ovarian estrogen, de novo synthesis of estrogen in the uterus is essential for the progress of decidualization. However, the molecular mechanisms are not fully understood. Studies have shown that Cystatin B (Cstb) is highly expressed in the decidual tissue of the uterus, but the regulation and mechanism of Cstb in the process of decidualization have not been reported. Our results showed that Cstb was highly expressed in mouse decidua and artificially induced deciduoma via in situ hybridization and immunofluorescence. Estrogen stimulates the expression of Cstb through the Estrogen receptor (ER)α. Moreover, in situ synthesis of estrogen in the uterus during decidualization regulates the expression of Cstb. Silencing the expression of Cstb affects the migration ability of stromal cells. Knockdown Cstb by siRNA significantly inhibits the expression of Dtprp, a marker for mouse decidualization. Our study identifies a novel estrogen target, Cstb, during decidualization and reveals that Cstb may play a pivotal role in angiogenesis during mouse decidualization via the Angptl7.
(Less)
- author
- Lu, Lin
; Chen, Yingni
; Yang, Zhenshan
LU
; Liang, Shijin
; Zhu, Songqi
and Liang, Xiaohuan
- publishing date
- 2023-01
- type
- Contribution to journal
- publication status
- published
- keywords
- Cstb, decidualization, embryo implantation, estrogen, uterus
- in
- International Journal of Molecular Sciences
- volume
- 24
- issue
- 1
- article number
- 302
- publisher
- MDPI AG
- external identifiers
-
- pmid:36613747
- scopus:85145979262
- ISSN
- 1661-6596
- DOI
- 10.3390/ijms24010302
- language
- English
- LU publication?
- no
- additional info
- Funding Information: This study was supported by the Guangdong Basic and Applied Basic Research Foundation (2019A1515010954 and 2021A1515012089). Publisher Copyright: © 2022 by the authors.
- id
- 6a7cbec4-cc9d-48a5-b2e8-c43c7e02e9fe
- date added to LUP
- 2024-02-28 14:56:47
- date last changed
- 2024-06-22 18:22:55
@article{6a7cbec4-cc9d-48a5-b2e8-c43c7e02e9fe,
abstract = {{<p>During decidualization in rodents, uterine stromal cells undergo extensive reprogramming to differentiate into distinct cell types, forming primary decidual zones (PDZs), secondary decidual zones (SDZs), and layers of undifferentiated stromal cells. The formation of secondary decidual zones is accompanied by extensive angiogenesis. During early pregnancy, besides ovarian estrogen, de novo synthesis of estrogen in the uterus is essential for the progress of decidualization. However, the molecular mechanisms are not fully understood. Studies have shown that Cystatin B (Cstb) is highly expressed in the decidual tissue of the uterus, but the regulation and mechanism of Cstb in the process of decidualization have not been reported. Our results showed that Cstb was highly expressed in mouse decidua and artificially induced deciduoma via in situ hybridization and immunofluorescence. Estrogen stimulates the expression of Cstb through the Estrogen receptor (ER)α. Moreover, in situ synthesis of estrogen in the uterus during decidualization regulates the expression of Cstb. Silencing the expression of Cstb affects the migration ability of stromal cells. Knockdown Cstb by siRNA significantly inhibits the expression of Dtprp, a marker for mouse decidualization. Our study identifies a novel estrogen target, Cstb, during decidualization and reveals that Cstb may play a pivotal role in angiogenesis during mouse decidualization via the Angptl7.</p>}},
author = {{Lu, Lin and Chen, Yingni and Yang, Zhenshan and Liang, Shijin and Zhu, Songqi and Liang, Xiaohuan}},
issn = {{1661-6596}},
keywords = {{Cstb; decidualization; embryo implantation; estrogen; uterus}},
language = {{eng}},
number = {{1}},
publisher = {{MDPI AG}},
series = {{International Journal of Molecular Sciences}},
title = {{Expression and Regulation of a Novel Decidual Cells-Derived Estrogen Target during Decidualization}},
url = {{http://dx.doi.org/10.3390/ijms24010302}},
doi = {{10.3390/ijms24010302}},
volume = {{24}},
year = {{2023}},
}