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Development of two-photon polymerised scaffolds for optical interrogation and neurite guidance of human iPSC-derived cortical neuronal networks

Crowe, J. A. LU orcid ; El-tamer, A. ; Nagel, D. ; Koroleva, A. V. ; Madrid-Wolff, J. ; Olarte, O. E. ; Sokolovsky, S. ; Estevez-priego, E. ; Ludl, A.-a. and Soriano, J. , et al. (2020) In Lab on a Chip 20(10). p.1792-1806
Abstract
Recent progress in the field of human induced pluripotent stem cells (iPSCs) has led to the efficient production of human neuronal cell models for in vitro study. This has the potential to enable the understanding of live human cellular and network function which is otherwise not possible. However, a major challenge is the generation of reproducible neural networks together with the ability to interrogate and record at the single cell level. A promising aid is the use of biomaterial scaffolds that would enable the development and guidance of neuronal networks in physiologically relevant architectures and dimensionality. The optimal scaffold material would need to be precisely fabricated with submicron resolution, be optically transparent,... (More)
Recent progress in the field of human induced pluripotent stem cells (iPSCs) has led to the efficient production of human neuronal cell models for in vitro study. This has the potential to enable the understanding of live human cellular and network function which is otherwise not possible. However, a major challenge is the generation of reproducible neural networks together with the ability to interrogate and record at the single cell level. A promising aid is the use of biomaterial scaffolds that would enable the development and guidance of neuronal networks in physiologically relevant architectures and dimensionality. The optimal scaffold material would need to be precisely fabricated with submicron resolution, be optically transparent, and biocompatible. Two-photon polymerisation (2PP) enables precise microfabrication of three-dimensional structures. In this study, we report the identification of two biomaterials that support the growth and differentiation of human iPSC-derived neural progenitors into functional neuronal networks. Furthermore, these materials can be patterned to induce alignment of neuronal processes and enable the optical interrogation of individual cells. 2PP scaffolds with tailored topographies therefore provide an effective method of producing defined in vitro human neural networks for application in influencing neurite guidance and complex network activity. (Less)
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publishing date
type
Contribution to journal
publication status
published
keywords
iPSCs, biomaterial, neuroscience
in
Lab on a Chip
volume
20
issue
10
pages
1792 - 1806
publisher
Royal Society of Chemistry
external identifiers
  • scopus:85084943122
ISSN
1473-0189
DOI
10.1039/C9LC01209E
language
English
LU publication?
no
id
6aaff473-4ed4-4711-a98b-705fe1390331
date added to LUP
2021-11-26 14:30:16
date last changed
2022-04-27 06:05:47
@article{6aaff473-4ed4-4711-a98b-705fe1390331,
  abstract     = {{Recent progress in the field of human induced pluripotent stem cells (iPSCs) has led to the efficient production of human neuronal cell models for in vitro study. This has the potential to enable the understanding of live human cellular and network function which is otherwise not possible. However, a major challenge is the generation of reproducible neural networks together with the ability to interrogate and record at the single cell level. A promising aid is the use of biomaterial scaffolds that would enable the development and guidance of neuronal networks in physiologically relevant architectures and dimensionality. The optimal scaffold material would need to be precisely fabricated with submicron resolution, be optically transparent, and biocompatible. Two-photon polymerisation (2PP) enables precise microfabrication of three-dimensional structures. In this study, we report the identification of two biomaterials that support the growth and differentiation of human iPSC-derived neural progenitors into functional neuronal networks. Furthermore, these materials can be patterned to induce alignment of neuronal processes and enable the optical interrogation of individual cells. 2PP scaffolds with tailored topographies therefore provide an effective method of producing defined in vitro human neural networks for application in influencing neurite guidance and complex network activity.}},
  author       = {{Crowe, J. A. and El-tamer, A. and Nagel, D. and Koroleva, A. V. and Madrid-Wolff, J. and Olarte, O. E. and Sokolovsky, S. and Estevez-priego, E. and Ludl, A.-a. and Soriano, J. and Loza-alvarez, P. and Chichkov, B. N. and Hill, E. J. and Parri, H. R. and Rafailov, E. U.}},
  issn         = {{1473-0189}},
  keywords     = {{iPSCs; biomaterial; neuroscience}},
  language     = {{eng}},
  number       = {{10}},
  pages        = {{1792--1806}},
  publisher    = {{Royal Society of Chemistry}},
  series       = {{Lab on a Chip}},
  title        = {{Development of two-photon polymerised scaffolds for optical interrogation and neurite guidance of human iPSC-derived cortical neuronal networks}},
  url          = {{http://dx.doi.org/10.1039/C9LC01209E}},
  doi          = {{10.1039/C9LC01209E}},
  volume       = {{20}},
  year         = {{2020}},
}