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Ecotoxicological assessment of propiconazole using soil bacterial and fungal growth assays

Fernández-Calviño, David LU ; Rousk, Johannes LU ; Bååth, Erland LU ; Bollmann, Ulla Elisabeth; Bester, Kai and Brandt, Kristian Koefoed (2017) In Applied Soil Ecology 115. p.27-30
Abstract

Effects of the fungicide propiconazole on soil microorganisms were tested using [3H] leucine incorporation and [14C] acetate in ergosterol incorporation to measure bacterial and fungal growth inhibition, respectively. Growth was compared to basal respiration (BR) and substrate-induced respiration (SIR) in soil microcosms established according to the OECD 217 guideline. Fungal growth was most sensitive with IC50 values remaining around 300 mg kg−1 during 40 days of incubation. SIR was initially less sensitive (IC50 1300 mg kg−1), but IC50 values progressively decreased over time to reach 380 mg kg−1 after 40 days. Bacterial growth was affected at... (More)

Effects of the fungicide propiconazole on soil microorganisms were tested using [3H] leucine incorporation and [14C] acetate in ergosterol incorporation to measure bacterial and fungal growth inhibition, respectively. Growth was compared to basal respiration (BR) and substrate-induced respiration (SIR) in soil microcosms established according to the OECD 217 guideline. Fungal growth was most sensitive with IC50 values remaining around 300 mg kg−1 during 40 days of incubation. SIR was initially less sensitive (IC50 1300 mg kg−1), but IC50 values progressively decreased over time to reach 380 mg kg−1 after 40 days. Bacterial growth was affected at concentrations ≥200 mg kg−1, but exhibited more complex dose-response relationships possibly due to a combination of direct toxicity, bacterial community adaptation, and competitive release from the more severely affected fungi. BR was either stimulated or not affected by propiconazole. Our results indicate that group-specific endpoints targeting microbial growth will improve ecotoxicological assessment of toxicants for environmental risk assessment.

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organization
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Contribution to journal
publication status
published
subject
keywords
Biocides, Ecotoxicology, Fungicides, Microbial activity, Soil microorganisms
in
Applied Soil Ecology
volume
115
pages
4 pages
publisher
Elsevier
external identifiers
  • scopus:85016498566
  • wos:000401881200004
ISSN
0929-1393
DOI
10.1016/j.apsoil.2017.03.009
language
English
LU publication?
yes
id
6afe5b77-23b6-41c9-bf3b-01f7c5cf661d
date added to LUP
2017-04-11 09:51:14
date last changed
2017-09-18 13:32:04
@article{6afe5b77-23b6-41c9-bf3b-01f7c5cf661d,
  abstract     = {<p>Effects of the fungicide propiconazole on soil microorganisms were tested using [<sup>3</sup>H] leucine incorporation and [<sup>14</sup>C] acetate in ergosterol incorporation to measure bacterial and fungal growth inhibition, respectively. Growth was compared to basal respiration (BR) and substrate-induced respiration (SIR) in soil microcosms established according to the OECD 217 guideline. Fungal growth was most sensitive with IC<sub>50</sub> values remaining around 300 mg kg<sup>−1</sup> during 40 days of incubation. SIR was initially less sensitive (IC<sub>50</sub> 1300 mg kg<sup>−1</sup>), but IC<sub>50</sub> values progressively decreased over time to reach 380 mg kg<sup>−1</sup> after 40 days. Bacterial growth was affected at concentrations ≥200 mg kg<sup>−1</sup>, but exhibited more complex dose-response relationships possibly due to a combination of direct toxicity, bacterial community adaptation, and competitive release from the more severely affected fungi. BR was either stimulated or not affected by propiconazole. Our results indicate that group-specific endpoints targeting microbial growth will improve ecotoxicological assessment of toxicants for environmental risk assessment.</p>},
  author       = {Fernández-Calviño, David and Rousk, Johannes and Bååth, Erland and Bollmann, Ulla Elisabeth and Bester, Kai and Brandt, Kristian Koefoed},
  issn         = {0929-1393},
  keyword      = {Biocides,Ecotoxicology,Fungicides,Microbial activity,Soil microorganisms},
  language     = {eng},
  month        = {07},
  pages        = {27--30},
  publisher    = {Elsevier},
  series       = {Applied Soil Ecology},
  title        = {Ecotoxicological assessment of propiconazole using soil bacterial and fungal growth assays},
  url          = {http://dx.doi.org/10.1016/j.apsoil.2017.03.009},
  volume       = {115},
  year         = {2017},
}