Ecotoxicological assessment of propiconazole using soil bacterial and fungal growth assays
(2017) In Applied Soil Ecology 115. p.27-30- Abstract
Effects of the fungicide propiconazole on soil microorganisms were tested using [3H] leucine incorporation and [14C] acetate in ergosterol incorporation to measure bacterial and fungal growth inhibition, respectively. Growth was compared to basal respiration (BR) and substrate-induced respiration (SIR) in soil microcosms established according to the OECD 217 guideline. Fungal growth was most sensitive with IC50 values remaining around 300 mg kg−1 during 40 days of incubation. SIR was initially less sensitive (IC50 1300 mg kg−1), but IC50 values progressively decreased over time to reach 380 mg kg−1 after 40 days. Bacterial growth was affected at... (More)
Effects of the fungicide propiconazole on soil microorganisms were tested using [3H] leucine incorporation and [14C] acetate in ergosterol incorporation to measure bacterial and fungal growth inhibition, respectively. Growth was compared to basal respiration (BR) and substrate-induced respiration (SIR) in soil microcosms established according to the OECD 217 guideline. Fungal growth was most sensitive with IC50 values remaining around 300 mg kg−1 during 40 days of incubation. SIR was initially less sensitive (IC50 1300 mg kg−1), but IC50 values progressively decreased over time to reach 380 mg kg−1 after 40 days. Bacterial growth was affected at concentrations ≥200 mg kg−1, but exhibited more complex dose-response relationships possibly due to a combination of direct toxicity, bacterial community adaptation, and competitive release from the more severely affected fungi. BR was either stimulated or not affected by propiconazole. Our results indicate that group-specific endpoints targeting microbial growth will improve ecotoxicological assessment of toxicants for environmental risk assessment.
(Less)
- author
- Fernández-Calviño, David LU ; Rousk, Johannes LU ; Bååth, Erland LU ; Bollmann, Ulla Elisabeth ; Bester, Kai and Brandt, Kristian Koefoed
- organization
- publishing date
- 2017-07-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Biocides, Ecotoxicology, Fungicides, Microbial activity, Soil microorganisms
- in
- Applied Soil Ecology
- volume
- 115
- pages
- 4 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:85016498566
- wos:000401881200004
- ISSN
- 0929-1393
- DOI
- 10.1016/j.apsoil.2017.03.009
- language
- English
- LU publication?
- yes
- id
- 6afe5b77-23b6-41c9-bf3b-01f7c5cf661d
- date added to LUP
- 2017-04-11 09:51:14
- date last changed
- 2024-04-14 09:13:35
@article{6afe5b77-23b6-41c9-bf3b-01f7c5cf661d,
abstract = {{<p>Effects of the fungicide propiconazole on soil microorganisms were tested using [<sup>3</sup>H] leucine incorporation and [<sup>14</sup>C] acetate in ergosterol incorporation to measure bacterial and fungal growth inhibition, respectively. Growth was compared to basal respiration (BR) and substrate-induced respiration (SIR) in soil microcosms established according to the OECD 217 guideline. Fungal growth was most sensitive with IC<sub>50</sub> values remaining around 300 mg kg<sup>−1</sup> during 40 days of incubation. SIR was initially less sensitive (IC<sub>50</sub> 1300 mg kg<sup>−1</sup>), but IC<sub>50</sub> values progressively decreased over time to reach 380 mg kg<sup>−1</sup> after 40 days. Bacterial growth was affected at concentrations ≥200 mg kg<sup>−1</sup>, but exhibited more complex dose-response relationships possibly due to a combination of direct toxicity, bacterial community adaptation, and competitive release from the more severely affected fungi. BR was either stimulated or not affected by propiconazole. Our results indicate that group-specific endpoints targeting microbial growth will improve ecotoxicological assessment of toxicants for environmental risk assessment.</p>}},
author = {{Fernández-Calviño, David and Rousk, Johannes and Bååth, Erland and Bollmann, Ulla Elisabeth and Bester, Kai and Brandt, Kristian Koefoed}},
issn = {{0929-1393}},
keywords = {{Biocides; Ecotoxicology; Fungicides; Microbial activity; Soil microorganisms}},
language = {{eng}},
month = {{07}},
pages = {{27--30}},
publisher = {{Elsevier}},
series = {{Applied Soil Ecology}},
title = {{Ecotoxicological assessment of propiconazole using soil bacterial and fungal growth assays}},
url = {{http://dx.doi.org/10.1016/j.apsoil.2017.03.009}},
doi = {{10.1016/j.apsoil.2017.03.009}},
volume = {{115}},
year = {{2017}},
}