A solid state transition in the tetragonal lipid bilayer structure at the lung alveolar surface
(2003) In Solid State Sciences 5(1). p.109-114- Abstract
- According to our recent results the alveolar surface is formed by a coherent phase, not a monolayer as has been assumed earlier. This surface phase is a tetragonal organization of the lipid bilayer which seems to follow the CLP minimal surface structure. As lipid bilayers at cooling will undergo a transition from the liquid-crystalline type of structure into a solid state structure, we have followed the changes in the X-ray scattering curves versus temperature of a sample of lung lavage from rabbit. There are significant changes in the range 15-25 degreesC indicating a solid/liquid bilayer transition. The size of the X-ray scattering changes indicate that only parts of the bilayers are involved. As indicated by similar studies of lung... (More)
- According to our recent results the alveolar surface is formed by a coherent phase, not a monolayer as has been assumed earlier. This surface phase is a tetragonal organization of the lipid bilayer which seems to follow the CLP minimal surface structure. As lipid bilayers at cooling will undergo a transition from the liquid-crystalline type of structure into a solid state structure, we have followed the changes in the X-ray scattering curves versus temperature of a sample of lung lavage from rabbit. There are significant changes in the range 15-25 degreesC indicating a solid/liquid bilayer transition. The size of the X-ray scattering changes indicate that only parts of the bilayers are involved. As indicated by similar studies of lung surfactant extracts, there are cholesterol-rich regions that remain in the liquid-like disordered conformation at cooling through this transition. The bilayer-embedded proteins in the alveolar CLP; structure are proposed to be located in the "corners" of the CLP-structure. This surface-phase structure was also examined by conventional electron microscopy with fixation at room temperature, and it was found to exhibit quite planar bilayer regions. On the other side, the same-sample seen in cryo-transmission electron microscopy vitrified from 40 degreesC was more disordered. These observations are consistent with a partial solid state transition of the bilayer on cooling, with segregation of lipids within the bilayers into cholesterol-rich regions remaining in a liquid-like disordered state and cholesterol-poor regions crystallizing in the range 25-15 degreesC. Physiological consequences-of such a partial solidification are finally discussed. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/312372
- author
- Larsson, Marcus LU ; Terasaki, O and Larsson, Kåre LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- solid/liquid transition, lipid bilayer transition, lung surfactant, tubular myelin
- in
- Solid State Sciences
- volume
- 5
- issue
- 1
- pages
- 109 - 114
- publisher
- Elsevier
- external identifiers
-
- wos:000182558200010
- scopus:0037279623
- ISSN
- 1873-3085
- DOI
- 10.1016/S1293-2558(02)00084-5
- language
- English
- LU publication?
- yes
- id
- 6b4cc0da-94db-4570-b5a9-c909f5d3b552 (old id 312372)
- date added to LUP
- 2016-04-01 12:28:36
- date last changed
- 2022-01-27 05:37:37
@article{6b4cc0da-94db-4570-b5a9-c909f5d3b552,
abstract = {{According to our recent results the alveolar surface is formed by a coherent phase, not a monolayer as has been assumed earlier. This surface phase is a tetragonal organization of the lipid bilayer which seems to follow the CLP minimal surface structure. As lipid bilayers at cooling will undergo a transition from the liquid-crystalline type of structure into a solid state structure, we have followed the changes in the X-ray scattering curves versus temperature of a sample of lung lavage from rabbit. There are significant changes in the range 15-25 degreesC indicating a solid/liquid bilayer transition. The size of the X-ray scattering changes indicate that only parts of the bilayers are involved. As indicated by similar studies of lung surfactant extracts, there are cholesterol-rich regions that remain in the liquid-like disordered conformation at cooling through this transition. The bilayer-embedded proteins in the alveolar CLP; structure are proposed to be located in the "corners" of the CLP-structure. This surface-phase structure was also examined by conventional electron microscopy with fixation at room temperature, and it was found to exhibit quite planar bilayer regions. On the other side, the same-sample seen in cryo-transmission electron microscopy vitrified from 40 degreesC was more disordered. These observations are consistent with a partial solid state transition of the bilayer on cooling, with segregation of lipids within the bilayers into cholesterol-rich regions remaining in a liquid-like disordered state and cholesterol-poor regions crystallizing in the range 25-15 degreesC. Physiological consequences-of such a partial solidification are finally discussed.}},
author = {{Larsson, Marcus and Terasaki, O and Larsson, Kåre}},
issn = {{1873-3085}},
keywords = {{solid/liquid transition; lipid bilayer transition; lung surfactant; tubular myelin}},
language = {{eng}},
number = {{1}},
pages = {{109--114}},
publisher = {{Elsevier}},
series = {{Solid State Sciences}},
title = {{A solid state transition in the tetragonal lipid bilayer structure at the lung alveolar surface}},
url = {{http://dx.doi.org/10.1016/S1293-2558(02)00084-5}},
doi = {{10.1016/S1293-2558(02)00084-5}},
volume = {{5}},
year = {{2003}},
}