IFN-α primes T- and NK-cells for IL-15-mediated signaling and cytotoxicity
Hansen, Mikkel L ; Woetmann, Anders ; Krejsgaard, Thorbjørn ; Kopp, Katharina L M ; Søkilde, Rolf LU
; Litman, Thomas
; Straten, Per Th
; Geisler, Carsten
; Wasik, Mariusz A.
and Odum, Niels
, et al.
(2011)
In Molecular Immunology
48(15-16).
p.93-2087
- Abstract
Recently it has become clear that interferon (IFN)-α, a type I interferon produced rapidly in response to infection, not only plays a key role in innate immunity, but also promotes adaptive immune responses by influencing the production or function of other cytokines. During infections IFN-α fosters the production of IL-15, which plays a pivotal role in the development, survival and function of NK cells and recruitment and activation of T cells. Since these two cytokines exert overlapping functions during infections, this investigation was undertaken to study the priming effect of IFN-α on the effect of IL-15 on human T and NK cells. We show that IFN-α induces an increased expression of IL-15Rα in human activated peripheral T cells, and... (More)
Recently it has become clear that interferon (IFN)-α, a type I interferon produced rapidly in response to infection, not only plays a key role in innate immunity, but also promotes adaptive immune responses by influencing the production or function of other cytokines. During infections IFN-α fosters the production of IL-15, which plays a pivotal role in the development, survival and function of NK cells and recruitment and activation of T cells. Since these two cytokines exert overlapping functions during infections, this investigation was undertaken to study the priming effect of IFN-α on the effect of IL-15 on human T and NK cells. We show that IFN-α induces an increased expression of IL-15Rα in human activated peripheral T cells, and in CD8(+) and CD4(+) T-cell lines. Functionally, the IFN-α-enhanced IL-15Rα expression resulted in an enhanced IL-15-mediated phosphorylation of STAT5 and STAT3 followed by a further increase in IL-15Rα expression. Moreover, IFN-α significantly increased the IL-15-induced cytotoxic activity of freshly isolated T and NK cells. Taken together, our data show that IFN-α boosts signaling and functional effects of IL-15, at least in part by fostering the increased IL-15R expression, thus add new facet to the emerging role of IFN-α as an important primer of adaptive immune responses.
(Less)
- author
- Hansen, Mikkel L
; Woetmann, Anders
; Krejsgaard, Thorbjørn
; Kopp, Katharina L M
; Søkilde, Rolf
LU
; Litman, Thomas
; Straten, Per Th
; Geisler, Carsten
; Wasik, Mariusz A.
and Odum, Niels
, et al. (More)
- Hansen, Mikkel L
; Woetmann, Anders
; Krejsgaard, Thorbjørn
; Kopp, Katharina L M
; Søkilde, Rolf
LU
; Litman, Thomas
; Straten, Per Th
; Geisler, Carsten
; Wasik, Mariusz A.
; Odum, Niels
and Eriksen, Karsten W
(Less)
- publishing date
- 2011-09
- type
- Contribution to journal
- publication status
- published
- keywords
- Blotting, Western, Cell Separation, Cells, Cultured, Cytotoxicity, Immunologic, Flow Cytometry, Humans, Interferon-alpha, Interleukin-15, Killer Cells, Natural, Lymphocyte Activation, Lymphocyte Culture Test, Mixed, Receptors, Interleukin-15, Recombinant Proteins, Signal Transduction, T-Lymphocytes, Journal Article, Research Support, Non-U.S. Gov't
- in
- Molecular Immunology
- volume
- 48
- issue
- 15-16
- pages
- 7 pages
- publisher
- Pergamon Press Ltd.
- external identifiers
-
- pmid:21813181
- scopus:80051918473
- ISSN
- 1872-9142
- DOI
- 10.1016/j.molimm.2011.07.008
- language
- English
- LU publication?
- no
- id
- 6b93e609-ff90-402c-9bd1-f41b72d8c171
- date added to LUP
- 2017-09-01 14:30:40
- date last changed
- 2024-06-09 22:48:40
@article{6b93e609-ff90-402c-9bd1-f41b72d8c171,
abstract = {{<p>Recently it has become clear that interferon (IFN)-α, a type I interferon produced rapidly in response to infection, not only plays a key role in innate immunity, but also promotes adaptive immune responses by influencing the production or function of other cytokines. During infections IFN-α fosters the production of IL-15, which plays a pivotal role in the development, survival and function of NK cells and recruitment and activation of T cells. Since these two cytokines exert overlapping functions during infections, this investigation was undertaken to study the priming effect of IFN-α on the effect of IL-15 on human T and NK cells. We show that IFN-α induces an increased expression of IL-15Rα in human activated peripheral T cells, and in CD8(+) and CD4(+) T-cell lines. Functionally, the IFN-α-enhanced IL-15Rα expression resulted in an enhanced IL-15-mediated phosphorylation of STAT5 and STAT3 followed by a further increase in IL-15Rα expression. Moreover, IFN-α significantly increased the IL-15-induced cytotoxic activity of freshly isolated T and NK cells. Taken together, our data show that IFN-α boosts signaling and functional effects of IL-15, at least in part by fostering the increased IL-15R expression, thus add new facet to the emerging role of IFN-α as an important primer of adaptive immune responses.</p>}},
author = {{Hansen, Mikkel L and Woetmann, Anders and Krejsgaard, Thorbjørn and Kopp, Katharina L M and Søkilde, Rolf and Litman, Thomas and Straten, Per Th and Geisler, Carsten and Wasik, Mariusz A. and Odum, Niels and Eriksen, Karsten W}},
issn = {{1872-9142}},
keywords = {{Blotting, Western; Cell Separation; Cells, Cultured; Cytotoxicity, Immunologic; Flow Cytometry; Humans; Interferon-alpha; Interleukin-15; Killer Cells, Natural; Lymphocyte Activation; Lymphocyte Culture Test, Mixed; Receptors, Interleukin-15; Recombinant Proteins; Signal Transduction; T-Lymphocytes; Journal Article; Research Support, Non-U.S. Gov't}},
language = {{eng}},
number = {{15-16}},
pages = {{93--2087}},
publisher = {{Pergamon Press Ltd.}},
series = {{Molecular Immunology}},
title = {{IFN-α primes T- and NK-cells for IL-15-mediated signaling and cytotoxicity}},
url = {{http://dx.doi.org/10.1016/j.molimm.2011.07.008}},
doi = {{10.1016/j.molimm.2011.07.008}},
volume = {{48}},
year = {{2011}},
}