Community Structure Evolution and Enrichment of Glycogen-Accumulating Organisms Producing Polyhydroxyalkanoates from Fermented Molasses
(2009) In Applied and Environmental Microbiology 75(14). p.4676-4686- Abstract
- An open mixed culture was enriched with glycogen-accumulating organisms (GAOs) by using a sequencing batch reactor and treating an agroindustrial waste (sugar cane molasses) under cyclic anaerobic-aerobic conditions. Over a 1-year operating period, the culture exhibited a very stable GAO phenotype with an average polyhydroxyalkanoate (PHA) content of 17% total suspended solids. However, the GAO microbial community evolved over the course of operation to a culture exhibiting unusual characteristics in producing PHAs comprised of short-chain-length monomers, namely, 3-hydroxybutyrate, 3-hydroxy-2- methylbutyrate, 3-hydroxyvalerate, and 3-hydroxy-2- methylvalerate, and also, up to 31 mol% of the medium-chain-length (MCL) monomer... (More)
- An open mixed culture was enriched with glycogen-accumulating organisms (GAOs) by using a sequencing batch reactor and treating an agroindustrial waste (sugar cane molasses) under cyclic anaerobic-aerobic conditions. Over a 1-year operating period, the culture exhibited a very stable GAO phenotype with an average polyhydroxyalkanoate (PHA) content of 17% total suspended solids. However, the GAO microbial community evolved over the course of operation to a culture exhibiting unusual characteristics in producing PHAs comprised of short-chain-length monomers, namely, 3-hydroxybutyrate, 3-hydroxy-2- methylbutyrate, 3-hydroxyvalerate, and 3-hydroxy-2- methylvalerate, and also, up to 31 mol% of the medium-chain-length (MCL) monomer 3-hydroxyhexanoate (3HHx). Microbial community analysis by fluorescence in situ hybridization revealed a concurrent long-term drift in the GAO community balance, from mainly "Candidatus Competibacter phosphatis" to mainly Defluviicoccus vanus-related organisms. The production of 3HHx was confirmed by C-13 nuclear magnetic resonance (NMR) and appeared to be related to the increased presence of D. vanus-related GAOs. These results suggest a broadened spectrum of material, chemical, and mechanical properties that can be achieved for biopolymers produced by open mixed cultures from fermented waste. The increased spectrum of polymer properties brings a wider scope of potential applications. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1412059
- author
- Pisco, Ana R. ; Bengtsson, Simon LU ; Werker, Alan ; Reis, Maria A. M. and Lemos, Paulo C.
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Applied and Environmental Microbiology
- volume
- 75
- issue
- 14
- pages
- 4676 - 4686
- publisher
- American Society for Microbiology
- external identifiers
-
- pmid:19465533
- scopus:67650498162
- wos:000267847000003
- ISSN
- 0099-2240
- DOI
- 10.1128/AEM.02486-08
- language
- English
- LU publication?
- yes
- id
- 6ba5aa5e-e165-4cb1-b21c-57e535223b9f (old id 1412059)
- date added to LUP
- 2016-04-01 11:33:28
- date last changed
- 2022-04-20 18:41:00
@article{6ba5aa5e-e165-4cb1-b21c-57e535223b9f,
abstract = {{An open mixed culture was enriched with glycogen-accumulating organisms (GAOs) by using a sequencing batch reactor and treating an agroindustrial waste (sugar cane molasses) under cyclic anaerobic-aerobic conditions. Over a 1-year operating period, the culture exhibited a very stable GAO phenotype with an average polyhydroxyalkanoate (PHA) content of 17% total suspended solids. However, the GAO microbial community evolved over the course of operation to a culture exhibiting unusual characteristics in producing PHAs comprised of short-chain-length monomers, namely, 3-hydroxybutyrate, 3-hydroxy-2- methylbutyrate, 3-hydroxyvalerate, and 3-hydroxy-2- methylvalerate, and also, up to 31 mol% of the medium-chain-length (MCL) monomer 3-hydroxyhexanoate (3HHx). Microbial community analysis by fluorescence in situ hybridization revealed a concurrent long-term drift in the GAO community balance, from mainly "Candidatus Competibacter phosphatis" to mainly Defluviicoccus vanus-related organisms. The production of 3HHx was confirmed by C-13 nuclear magnetic resonance (NMR) and appeared to be related to the increased presence of D. vanus-related GAOs. These results suggest a broadened spectrum of material, chemical, and mechanical properties that can be achieved for biopolymers produced by open mixed cultures from fermented waste. The increased spectrum of polymer properties brings a wider scope of potential applications.}},
author = {{Pisco, Ana R. and Bengtsson, Simon and Werker, Alan and Reis, Maria A. M. and Lemos, Paulo C.}},
issn = {{0099-2240}},
language = {{eng}},
number = {{14}},
pages = {{4676--4686}},
publisher = {{American Society for Microbiology}},
series = {{Applied and Environmental Microbiology}},
title = {{Community Structure Evolution and Enrichment of Glycogen-Accumulating Organisms Producing Polyhydroxyalkanoates from Fermented Molasses}},
url = {{http://dx.doi.org/10.1128/AEM.02486-08}},
doi = {{10.1128/AEM.02486-08}},
volume = {{75}},
year = {{2009}},
}