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Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation

Kurek, Magdalena ; Åkesson, Elisabet ; Yoshihara, Masahito ; Oliver, Elizabeth ; Cui, Yanhua ; Becker, Martin ; Alves-Lopes, João Pedro ; Bjarnason, Ragnar ; Romerius, Patrik LU and Sundin, Mikael , et al. (2021) In Cells 10(2).
Abstract

Fertility preservation for male childhood cancer survivors not yet capable of producing mature spermatozoa, relies on experimental approaches such as testicular explant culture. Although the first steps in somatic maturation can be observed in human testicular explant cultures, germ cell depletion is a common obstacle. Hence, understanding the spermatogonial stem cell (SSC) niche environment and in particular, specific components such as the seminiferous basement membrane (BM) will allow progression of testicular explant cultures. Here, we revealed that the seminiferous BM is established from 6 weeks post conception with the expression of laminin alpha 1 (LAMA 1) and type IV collagen, which persist as key components throughout... (More)

Fertility preservation for male childhood cancer survivors not yet capable of producing mature spermatozoa, relies on experimental approaches such as testicular explant culture. Although the first steps in somatic maturation can be observed in human testicular explant cultures, germ cell depletion is a common obstacle. Hence, understanding the spermatogonial stem cell (SSC) niche environment and in particular, specific components such as the seminiferous basement membrane (BM) will allow progression of testicular explant cultures. Here, we revealed that the seminiferous BM is established from 6 weeks post conception with the expression of laminin alpha 1 (LAMA 1) and type IV collagen, which persist as key components throughout development. With prepubertal testicular explant culture we found that seminiferous LAMA 1 expression is disrupted and depleted with culture time correlating with germ cell loss. These findings highlight the importance of LAMA 1 for the human SSC niche and its sensitivity to culture conditions.

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publishing date
type
Contribution to journal
publication status
published
subject
keywords
basal membrane, germ cells, infertility, late effects, seminiferous tubules, Sertoli cells, spermatogonia, stem cell niche
in
Cells
volume
10
issue
2
article number
241
publisher
MDPI AG
external identifiers
  • scopus:85100612669
  • pmid:33513766
ISSN
2073-4409
DOI
10.3390/cells10020241
language
English
LU publication?
yes
id
6bb406da-9186-4c5d-9605-9c26b36c2811
date added to LUP
2021-02-23 10:14:39
date last changed
2024-04-18 02:27:01
@article{6bb406da-9186-4c5d-9605-9c26b36c2811,
  abstract     = {{<p>Fertility preservation for male childhood cancer survivors not yet capable of producing mature spermatozoa, relies on experimental approaches such as testicular explant culture. Although the first steps in somatic maturation can be observed in human testicular explant cultures, germ cell depletion is a common obstacle. Hence, understanding the spermatogonial stem cell (SSC) niche environment and in particular, specific components such as the seminiferous basement membrane (BM) will allow progression of testicular explant cultures. Here, we revealed that the seminiferous BM is established from 6 weeks post conception with the expression of laminin alpha 1 (LAMA 1) and type IV collagen, which persist as key components throughout development. With prepubertal testicular explant culture we found that seminiferous LAMA 1 expression is disrupted and depleted with culture time correlating with germ cell loss. These findings highlight the importance of LAMA 1 for the human SSC niche and its sensitivity to culture conditions.</p>}},
  author       = {{Kurek, Magdalena and Åkesson, Elisabet and Yoshihara, Masahito and Oliver, Elizabeth and Cui, Yanhua and Becker, Martin and Alves-Lopes, João Pedro and Bjarnason, Ragnar and Romerius, Patrik and Sundin, Mikael and Norén Nyström, Ulrika and Langenskiöld, Cecilia and Vogt, Hartmut and Henningsohn, Lars and Petersen, Cecilia and Söder, Olle and Guo, Jingtao and Mitchell, Rod T. and Jahnukainen, Kirsi and Stukenborg, Jan Bernd}},
  issn         = {{2073-4409}},
  keywords     = {{basal membrane; germ cells; infertility; late effects; seminiferous tubules; Sertoli cells; spermatogonia; stem cell niche}},
  language     = {{eng}},
  number       = {{2}},
  publisher    = {{MDPI AG}},
  series       = {{Cells}},
  title        = {{Spermatogonia Loss Correlates with LAMA 1 Expression in Human Prepubertal Testes Stored for Fertility Preservation}},
  url          = {{http://dx.doi.org/10.3390/cells10020241}},
  doi          = {{10.3390/cells10020241}},
  volume       = {{10}},
  year         = {{2021}},
}