Biochemical and functional correlates of an increased membrane density of caveolae in hypertrophic rat urinary bladder.
(2010) In European Journal of Pharmacology 649. p.362-368- Abstract
- Organ hypertrophy is often found to be associated with changes in the expression of caveolins and altered density of caveolae in the membrane. A plethora of signalling intermediaries are associated with caveolae and loss of caveolae has profound effects on contractility of the urinary bladder. We hypothesized that smooth muscle hypertrophy caused by bladder outflow obstruction (BOO) might lead to an altered caveola density with consequences for contractile regulation. Rat BOO for 6weeks caused a 2.56-fold increase in the number of smooth muscle caveolae per μm membrane. No changes in the expression of caveolin-1 or cavin-1, normalized to β-actin were seen, but membrane area per unit muscle volume dropped to 0.346. Hypertrophy was... (More)
- Organ hypertrophy is often found to be associated with changes in the expression of caveolins and altered density of caveolae in the membrane. A plethora of signalling intermediaries are associated with caveolae and loss of caveolae has profound effects on contractility of the urinary bladder. We hypothesized that smooth muscle hypertrophy caused by bladder outflow obstruction (BOO) might lead to an altered caveola density with consequences for contractile regulation. Rat BOO for 6weeks caused a 2.56-fold increase in the number of smooth muscle caveolae per μm membrane. No changes in the expression of caveolin-1 or cavin-1, normalized to β-actin were seen, but membrane area per unit muscle volume dropped to 0.346. Hypertrophy was associated with altered contraction in response to carbachol. The effect on contraction of cholesterol desorption, which disrupts lipid rafts and caveolae, was however not changed. Contraction in response to bradykinin resisted mβcd in control destrusor, but was inhibited by it after 6weeks of obstruction. It is concluded that rat detrusor hypertrophy leads to an increased number of caveolae per unit membrane area. This change is due to a reduction of membrane area per volume muscle and it does not play a role for cholinergic activation, but promotes contraction in response to bradykinin after long-term obstruction. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1687821
- author
- Shakirova, Yulia LU ; Swärd, Karl LU ; Uvelius, Bengt LU and Ekman, Mari LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- European Journal of Pharmacology
- volume
- 649
- pages
- 362 - 368
- publisher
- Elsevier
- external identifiers
-
- wos:000284294000050
- pmid:20868660
- scopus:78049282840
- pmid:20868660
- ISSN
- 1879-0712
- DOI
- 10.1016/j.ejphar.2010.09.050
- language
- English
- LU publication?
- yes
- id
- 6bd8e7dc-e5ca-4afd-ab63-3922a88f751b (old id 1687821)
- alternative location
- http://lup.lub.lu.se/record/1687821/file/1693747.pdf
- http://www.ncbi.nlm.nih.gov/pubmed/20868660?dopt=Abstract
- date added to LUP
- 2016-04-04 08:53:54
- date last changed
- 2022-01-29 07:28:03
@article{6bd8e7dc-e5ca-4afd-ab63-3922a88f751b,
abstract = {{Organ hypertrophy is often found to be associated with changes in the expression of caveolins and altered density of caveolae in the membrane. A plethora of signalling intermediaries are associated with caveolae and loss of caveolae has profound effects on contractility of the urinary bladder. We hypothesized that smooth muscle hypertrophy caused by bladder outflow obstruction (BOO) might lead to an altered caveola density with consequences for contractile regulation. Rat BOO for 6weeks caused a 2.56-fold increase in the number of smooth muscle caveolae per μm membrane. No changes in the expression of caveolin-1 or cavin-1, normalized to β-actin were seen, but membrane area per unit muscle volume dropped to 0.346. Hypertrophy was associated with altered contraction in response to carbachol. The effect on contraction of cholesterol desorption, which disrupts lipid rafts and caveolae, was however not changed. Contraction in response to bradykinin resisted mβcd in control destrusor, but was inhibited by it after 6weeks of obstruction. It is concluded that rat detrusor hypertrophy leads to an increased number of caveolae per unit membrane area. This change is due to a reduction of membrane area per volume muscle and it does not play a role for cholinergic activation, but promotes contraction in response to bradykinin after long-term obstruction.}},
author = {{Shakirova, Yulia and Swärd, Karl and Uvelius, Bengt and Ekman, Mari}},
issn = {{1879-0712}},
language = {{eng}},
pages = {{362--368}},
publisher = {{Elsevier}},
series = {{European Journal of Pharmacology}},
title = {{Biochemical and functional correlates of an increased membrane density of caveolae in hypertrophic rat urinary bladder.}},
url = {{http://dx.doi.org/10.1016/j.ejphar.2010.09.050}},
doi = {{10.1016/j.ejphar.2010.09.050}},
volume = {{649}},
year = {{2010}},
}