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Dual function of the tRNA(m(5)U54)methyltransferase in tRNA maturation

Johansson, Marcus J O LU and Byström, Anders S (2002) In RNA 8(3). p.35-324
Abstract

A 5-methyluridine (m(5)U) residue at position 54 is a conserved feature of bacterial and eukaryotic tRNAs. The methylation of U54 is catalyzed by the tRNA(m5U54)methyltransferase, which in Saccharomyces cerevisiae is encoded by the nonessential TRM2 gene. In this study, we identified four different strains with mutant forms of tRNA(Ser)CGA. The absence of the TRM2 gene in these strains decreased the stability of tRNA(Ser)CGA and induced lethality. Two alleles of TRM2 encoding catalytically inactive tRNA(m5U54)methyltransferases were able to stabilize tRNA(Ser)CGA in one of the mutants, revealing a role for the Trm2 protein per se in tRNA maturation. Other tRNA modification enzymes interacting with tRNA(Ser)CGA in the maturation process,... (More)

A 5-methyluridine (m(5)U) residue at position 54 is a conserved feature of bacterial and eukaryotic tRNAs. The methylation of U54 is catalyzed by the tRNA(m5U54)methyltransferase, which in Saccharomyces cerevisiae is encoded by the nonessential TRM2 gene. In this study, we identified four different strains with mutant forms of tRNA(Ser)CGA. The absence of the TRM2 gene in these strains decreased the stability of tRNA(Ser)CGA and induced lethality. Two alleles of TRM2 encoding catalytically inactive tRNA(m5U54)methyltransferases were able to stabilize tRNA(Ser)CGA in one of the mutants, revealing a role for the Trm2 protein per se in tRNA maturation. Other tRNA modification enzymes interacting with tRNA(Ser)CGA in the maturation process, such as Pus4p, Trm1 p, and Trm3p were essential or important for growth of the tRNA(Ser)CGA mutants. Moreover, Lhp1p, a protein binding RNA polymerase III transcripts, was required to stabilize the mutant tRNAs. Based on our results, we suggest that tRNA modification enzymes might have a role in tRNA maturation not necessarily linked to their known catalytic activity.

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publication status
published
keywords
Antigens, Viral/metabolism, Base Sequence, Blotting, Northern, Cell Survival/physiology, Chromatography, High Pressure Liquid, Cloning, Molecular, DNA Primers/chemistry, Fungal Proteins/genetics, Molecular Sequence Data, Mutation/physiology, Phenotype, Plasmids, Polymerase Chain Reaction, RNA, Transfer, Ser/analysis, Saccharomyces cerevisiae/enzymology, Uridine/analogs & derivatives, tRNA Methyltransferases/genetics
in
RNA
volume
8
issue
3
pages
35 - 324
publisher
Cold Spring Harbor Laboratory Press (CSHL)
external identifiers
  • scopus:0036226647
  • pmid:12003492
ISSN
1355-8382
DOI
10.1017/s1355838202027851
language
English
LU publication?
no
id
6c3e46ae-27d2-4dcb-b0ee-b4ede008759d
date added to LUP
2024-03-05 17:03:45
date last changed
2024-03-12 11:47:41
@article{6c3e46ae-27d2-4dcb-b0ee-b4ede008759d,
  abstract     = {{<p>A 5-methyluridine (m(5)U) residue at position 54 is a conserved feature of bacterial and eukaryotic tRNAs. The methylation of U54 is catalyzed by the tRNA(m5U54)methyltransferase, which in Saccharomyces cerevisiae is encoded by the nonessential TRM2 gene. In this study, we identified four different strains with mutant forms of tRNA(Ser)CGA. The absence of the TRM2 gene in these strains decreased the stability of tRNA(Ser)CGA and induced lethality. Two alleles of TRM2 encoding catalytically inactive tRNA(m5U54)methyltransferases were able to stabilize tRNA(Ser)CGA in one of the mutants, revealing a role for the Trm2 protein per se in tRNA maturation. Other tRNA modification enzymes interacting with tRNA(Ser)CGA in the maturation process, such as Pus4p, Trm1 p, and Trm3p were essential or important for growth of the tRNA(Ser)CGA mutants. Moreover, Lhp1p, a protein binding RNA polymerase III transcripts, was required to stabilize the mutant tRNAs. Based on our results, we suggest that tRNA modification enzymes might have a role in tRNA maturation not necessarily linked to their known catalytic activity.</p>}},
  author       = {{Johansson, Marcus J O and Byström, Anders S}},
  issn         = {{1355-8382}},
  keywords     = {{Antigens, Viral/metabolism; Base Sequence; Blotting, Northern; Cell Survival/physiology; Chromatography, High Pressure Liquid; Cloning, Molecular; DNA Primers/chemistry; Fungal Proteins/genetics; Molecular Sequence Data; Mutation/physiology; Phenotype; Plasmids; Polymerase Chain Reaction; RNA, Transfer, Ser/analysis; Saccharomyces cerevisiae/enzymology; Uridine/analogs & derivatives; tRNA Methyltransferases/genetics}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{35--324}},
  publisher    = {{Cold Spring Harbor Laboratory Press (CSHL)}},
  series       = {{RNA}},
  title        = {{Dual function of the tRNA(m(5)U54)methyltransferase in tRNA maturation}},
  url          = {{http://dx.doi.org/10.1017/s1355838202027851}},
  doi          = {{10.1017/s1355838202027851}},
  volume       = {{8}},
  year         = {{2002}},
}