Formate Utilization by the Crenarchaeon Desulfurococcus amylolyticus
Ergal, Ipek ; Reischl, Barbara ; Hasibar, Benedikt ; Manoharan, Lokeshwaran LU
; Zipperle, Aaron
; Bochmann, Günther
; Fuchs, Werner
and Rittmann, Simon K-M R
(2020)
In Microorganisms
8(3).
- Abstract
Formate is one of the key compounds of the microbial carbon and/or energy metabolism. It owes a significant contribution to various anaerobic syntrophic associations, and may become one of the energy storage compounds of modern energy biotechnology. Microbial growth on formate was demonstrated for different bacteria and archaea, but not yet for species of the archaeal phylum Crenarchaeota. Here, we show that Desulfurococcus amylolyticus DSM 16532, an anaerobic and hyperthermophilic Crenarchaeon, metabolises formate without the production of molecular hydrogen. Growth, substrate uptake, and production kinetics on formate, glucose, and glucose/formate mixtures exhibited similar specific growth rates and similar final cell densities. A... (More)
Formate is one of the key compounds of the microbial carbon and/or energy metabolism. It owes a significant contribution to various anaerobic syntrophic associations, and may become one of the energy storage compounds of modern energy biotechnology. Microbial growth on formate was demonstrated for different bacteria and archaea, but not yet for species of the archaeal phylum Crenarchaeota. Here, we show that Desulfurococcus amylolyticus DSM 16532, an anaerobic and hyperthermophilic Crenarchaeon, metabolises formate without the production of molecular hydrogen. Growth, substrate uptake, and production kinetics on formate, glucose, and glucose/formate mixtures exhibited similar specific growth rates and similar final cell densities. A whole cell conversion experiment on formate revealed that D. amylolyticus converts formate into carbon dioxide, acetate, citrate, and ethanol. Using bioinformatic analysis, we examined whether one of the currently known and postulated formate utilisation pathways could be operative in D. amylolyticus. This analysis indicated the possibility that D. amylolyticus uses formaldehyde producing enzymes for the assimilation of formate. Therefore, we propose that formate might be assimilated into biomass through formaldehyde dehydrogenase and the oxidative pentose phosphate pathway. These findings shed new light on the metabolic versatility of the archaeal phylum Crenarchaeota.
(Less)
- author
- Ergal, Ipek
; Reischl, Barbara
; Hasibar, Benedikt
; Manoharan, Lokeshwaran
LU
; Zipperle, Aaron
; Bochmann, Günther
; Fuchs, Werner
and Rittmann, Simon K-M R
- publishing date
- 2020-03-23
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Microorganisms
- volume
- 8
- issue
- 3
- article number
- 454
- publisher
- MDPI AG
- external identifiers
-
- scopus:85083440513
- pmid:32210133
- ISSN
- 2076-2607
- DOI
- 10.3390/microorganisms8030454
- language
- English
- LU publication?
- no
- id
- 6c411a30-1f76-4cba-8d66-765a1dd62f5a
- date added to LUP
- 2020-03-31 09:56:34
- date last changed
- 2024-09-18 21:18:21
@article{6c411a30-1f76-4cba-8d66-765a1dd62f5a,
abstract = {{<p>Formate is one of the key compounds of the microbial carbon and/or energy metabolism. It owes a significant contribution to various anaerobic syntrophic associations, and may become one of the energy storage compounds of modern energy biotechnology. Microbial growth on formate was demonstrated for different bacteria and archaea, but not yet for species of the archaeal phylum Crenarchaeota. Here, we show that Desulfurococcus amylolyticus DSM 16532, an anaerobic and hyperthermophilic Crenarchaeon, metabolises formate without the production of molecular hydrogen. Growth, substrate uptake, and production kinetics on formate, glucose, and glucose/formate mixtures exhibited similar specific growth rates and similar final cell densities. A whole cell conversion experiment on formate revealed that D. amylolyticus converts formate into carbon dioxide, acetate, citrate, and ethanol. Using bioinformatic analysis, we examined whether one of the currently known and postulated formate utilisation pathways could be operative in D. amylolyticus. This analysis indicated the possibility that D. amylolyticus uses formaldehyde producing enzymes for the assimilation of formate. Therefore, we propose that formate might be assimilated into biomass through formaldehyde dehydrogenase and the oxidative pentose phosphate pathway. These findings shed new light on the metabolic versatility of the archaeal phylum Crenarchaeota.</p>}},
author = {{Ergal, Ipek and Reischl, Barbara and Hasibar, Benedikt and Manoharan, Lokeshwaran and Zipperle, Aaron and Bochmann, Günther and Fuchs, Werner and Rittmann, Simon K-M R}},
issn = {{2076-2607}},
language = {{eng}},
month = {{03}},
number = {{3}},
publisher = {{MDPI AG}},
series = {{Microorganisms}},
title = {{Formate Utilization by the Crenarchaeon Desulfurococcus amylolyticus}},
url = {{http://dx.doi.org/10.3390/microorganisms8030454}},
doi = {{10.3390/microorganisms8030454}},
volume = {{8}},
year = {{2020}},
}