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Mapping molecular complexes with super-resolution microscopy and single-particle analysis

Mendes, Afonso ; Heil, Hannah S LU orcid ; Coelho, Simao ; Leterrier, Christophe and Henriques, Ricardo (2022) In Open biology 12(7).
Abstract

Understanding the structure of supramolecular complexes provides insight into their functional capabilities and how they can be modulated in the context of disease. Super-resolution microscopy (SRM) excels in performing this task by resolving ultrastructural details at the nanoscale with molecular specificity. However, technical limitations, such as underlabelling, preclude its ability to provide complete structures. Single-particle analysis (SPA) overcomes this limitation by combining information from multiple images of identical structures and producing an averaged model, effectively enhancing the resolution and coverage of image reconstructions. This review highlights important studies using SRM-SPA, demonstrating how it broadens our... (More)

Understanding the structure of supramolecular complexes provides insight into their functional capabilities and how they can be modulated in the context of disease. Super-resolution microscopy (SRM) excels in performing this task by resolving ultrastructural details at the nanoscale with molecular specificity. However, technical limitations, such as underlabelling, preclude its ability to provide complete structures. Single-particle analysis (SPA) overcomes this limitation by combining information from multiple images of identical structures and producing an averaged model, effectively enhancing the resolution and coverage of image reconstructions. This review highlights important studies using SRM-SPA, demonstrating how it broadens our knowledge by elucidating features of key biological structures with unprecedented detail.

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Please use this url to cite or link to this publication:
author
; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Microscopy, Fluorescence/methods
in
Open biology
volume
12
issue
7
article number
220079
publisher
Royal Society Publishing
external identifiers
  • pmid:35892200
  • scopus:85135126050
ISSN
2046-2441
DOI
10.1098/rsob.220079
language
English
LU publication?
no
id
6ca333c7-a7f4-4cd7-a861-efdfe41c100b
date added to LUP
2025-04-26 11:49:00
date last changed
2025-06-22 08:13:37
@article{6ca333c7-a7f4-4cd7-a861-efdfe41c100b,
  abstract     = {{<p>Understanding the structure of supramolecular complexes provides insight into their functional capabilities and how they can be modulated in the context of disease. Super-resolution microscopy (SRM) excels in performing this task by resolving ultrastructural details at the nanoscale with molecular specificity. However, technical limitations, such as underlabelling, preclude its ability to provide complete structures. Single-particle analysis (SPA) overcomes this limitation by combining information from multiple images of identical structures and producing an averaged model, effectively enhancing the resolution and coverage of image reconstructions. This review highlights important studies using SRM-SPA, demonstrating how it broadens our knowledge by elucidating features of key biological structures with unprecedented detail.</p>}},
  author       = {{Mendes, Afonso and Heil, Hannah S and Coelho, Simao and Leterrier, Christophe and Henriques, Ricardo}},
  issn         = {{2046-2441}},
  keywords     = {{Microscopy, Fluorescence/methods}},
  language     = {{eng}},
  number       = {{7}},
  publisher    = {{Royal Society Publishing}},
  series       = {{Open biology}},
  title        = {{Mapping molecular complexes with super-resolution microscopy and single-particle analysis}},
  url          = {{http://dx.doi.org/10.1098/rsob.220079}},
  doi          = {{10.1098/rsob.220079}},
  volume       = {{12}},
  year         = {{2022}},
}