Advanced

Improved high-capacity adenoviral vectors for high-level neuron-restricted gene transfer to the CNS

Hermening, Stephan LU ; Kuegler, Sebastian ; Baehr, Mathias and Isenmann, Stefan (2006) In Journal of Virological Methods 136(1-2). p.30-37
Abstract
Adenovirus-based (Ad) vectors are used widely for experimental gene transfer to the CNS. Ad transduce many cell types including postmitotic neurons. However, their use for, CNS gene transfer is limited due to the host immune response elicited. Furthermore, the extensive distribution of the primary cellular receptor for Ad, the coxsackievirus and adenovirus receptor (CAR), allows adenoviral vectors to infect a broad range of host cells which may be disadvantageous in tissues with various different cell types, like the CNS. The use of tissue-specific promoters allows for neuron-restricted gene expression, even though gene expression driven by these promoters is often very weak. Accordingly, increased transgene expression levels from viral... (More)
Adenovirus-based (Ad) vectors are used widely for experimental gene transfer to the CNS. Ad transduce many cell types including postmitotic neurons. However, their use for, CNS gene transfer is limited due to the host immune response elicited. Furthermore, the extensive distribution of the primary cellular receptor for Ad, the coxsackievirus and adenovirus receptor (CAR), allows adenoviral vectors to infect a broad range of host cells which may be disadvantageous in tissues with various different cell types, like the CNS. The use of tissue-specific promoters allows for neuron-restricted gene expression, even though gene expression driven by these promoters is often very weak. Accordingly, increased transgene expression levels from viral transcription units are needed in order to improve the overall performance of Ad vectors. We designed a high-capacity Ad vector (HC-Ad) that allows for high-level, neuron-restricted transgene expression and shows no obvious signs of immumogenicity or toxicity in the mouse brain. (c) 2006 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
mice, neurodegeneration, genetic, gene transfer techniques, CNS diseases, adenoviridae, animal, vectors
in
Journal of Virological Methods
volume
136
issue
1-2
pages
30 - 37
publisher
Elsevier
external identifiers
  • pmid:16672163
  • wos:000239735200005
  • scopus:33745883543
  • pmid:16672163
ISSN
1879-0984
DOI
10.1016/j.jviromet.2006.03.031
language
English
LU publication?
yes
id
6ca36b61-fcf6-4b6b-9415-9e87aadfa767 (old id 397323)
date added to LUP
2016-04-01 11:34:07
date last changed
2020-06-10 02:16:43
@article{6ca36b61-fcf6-4b6b-9415-9e87aadfa767,
  abstract     = {Adenovirus-based (Ad) vectors are used widely for experimental gene transfer to the CNS. Ad transduce many cell types including postmitotic neurons. However, their use for, CNS gene transfer is limited due to the host immune response elicited. Furthermore, the extensive distribution of the primary cellular receptor for Ad, the coxsackievirus and adenovirus receptor (CAR), allows adenoviral vectors to infect a broad range of host cells which may be disadvantageous in tissues with various different cell types, like the CNS. The use of tissue-specific promoters allows for neuron-restricted gene expression, even though gene expression driven by these promoters is often very weak. Accordingly, increased transgene expression levels from viral transcription units are needed in order to improve the overall performance of Ad vectors. We designed a high-capacity Ad vector (HC-Ad) that allows for high-level, neuron-restricted transgene expression and shows no obvious signs of immumogenicity or toxicity in the mouse brain. (c) 2006 Elsevier B.V. All rights reserved.},
  author       = {Hermening, Stephan and Kuegler, Sebastian and Baehr, Mathias and Isenmann, Stefan},
  issn         = {1879-0984},
  language     = {eng},
  number       = {1-2},
  pages        = {30--37},
  publisher    = {Elsevier},
  series       = {Journal of Virological Methods},
  title        = {Improved high-capacity adenoviral vectors for high-level neuron-restricted gene transfer to the CNS},
  url          = {http://dx.doi.org/10.1016/j.jviromet.2006.03.031},
  doi          = {10.1016/j.jviromet.2006.03.031},
  volume       = {136},
  year         = {2006},
}