Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

[7] NMR studies of complex DNA structures : The holliday junction intermediate in genetic recombination

Carlström, Göran LU orcid ; Chen, Shiow-Meei ; Muck, Siobhan and Chazin, Walter J. (1995) In Methods in Enzymology 261. p.163-182
Abstract
Publisher Summary This chapter discusses the current status, of using nuclear magnetic resonance (NMR), to study the structure and dynamics of the holliday junction (HJ). Complex deoxyribonucleic acid (DNA) structures (e.g., triplexes, quadruplexes, junctions) pose difficult problems for study, by NMR, relative to the typical DNA duplexes, because they have nonstandard or distorted local conformations and higher molecular weights that give rise to large resonance linewidths and severe 1H spectral overlap. With more atoms in the system, both assignment and structure calculation become more challenging. The HJ, a four-arm DNA crossover structure, is a transient intermediate formed in the course of genetic recombination as well as during... (More)
Publisher Summary This chapter discusses the current status, of using nuclear magnetic resonance (NMR), to study the structure and dynamics of the holliday junction (HJ). Complex deoxyribonucleic acid (DNA) structures (e.g., triplexes, quadruplexes, junctions) pose difficult problems for study, by NMR, relative to the typical DNA duplexes, because they have nonstandard or distorted local conformations and higher molecular weights that give rise to large resonance linewidths and severe 1H spectral overlap. With more atoms in the system, both assignment and structure calculation become more challenging. The HJ, a four-arm DNA crossover structure, is a transient intermediate formed in the course of genetic recombination as well as during other cellular processes, such as replication and telomere resolution. A significant body of evidence has accumulated, indicating that the structure at the junction has a central role, in determining the outcome of these cellular events. For NMR studies, the titration of the four component 16-mer strands to create an equimolar mixture is critical. Gel electrophoresis has shown that titrations based on the standard ultraviolet (UV) estimates of strand concentrations result in significant amounts of residual single-strand, half-complementary duplex, and three-arm structures. (Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
host publication
Methods in Enzymology : Nuclear Magnetic Resonance and Nucleic Acids - Nuclear Magnetic Resonance and Nucleic Acids
series title
Methods in Enzymology
volume
261
pages
20 pages
publisher
Academic Press
external identifiers
  • scopus:0028872024
ISSN
0076-6879
ISBN
978-0-12-182162-3
DOI
10.1016/S0076-6879(95)61009-X
language
English
LU publication?
yes
id
6cc756c5-68bb-4005-92d8-77bd144d6dbf
alternative location
http://www.sciencedirect.com/science/article/pii/S007668799561009X
date added to LUP
2019-07-25 21:52:00
date last changed
2022-03-29 02:34:16
@inbook{6cc756c5-68bb-4005-92d8-77bd144d6dbf,
  abstract     = {{Publisher Summary This chapter discusses the current status, of using nuclear magnetic resonance (NMR), to study the structure and dynamics of the holliday junction (HJ). Complex deoxyribonucleic acid (DNA) structures (e.g., triplexes, quadruplexes, junctions) pose difficult problems for study, by NMR, relative to the typical DNA duplexes, because they have nonstandard or distorted local conformations and higher molecular weights that give rise to large resonance linewidths and severe 1H spectral overlap. With more atoms in the system, both assignment and structure calculation become more challenging. The HJ, a four-arm DNA crossover structure, is a transient intermediate formed in the course of genetic recombination as well as during other cellular processes, such as replication and telomere resolution. A significant body of evidence has accumulated, indicating that the structure at the junction has a central role, in determining the outcome of these cellular events. For NMR studies, the titration of the four component 16-mer strands to create an equimolar mixture is critical. Gel electrophoresis has shown that titrations based on the standard ultraviolet (UV) estimates of strand concentrations result in significant amounts of residual single-strand, half-complementary duplex, and three-arm structures.}},
  author       = {{Carlström, Göran and Chen, Shiow-Meei and Muck, Siobhan and Chazin, Walter J.}},
  booktitle    = {{Methods in Enzymology : Nuclear Magnetic Resonance and Nucleic Acids}},
  isbn         = {{978-0-12-182162-3}},
  issn         = {{0076-6879}},
  language     = {{eng}},
  pages        = {{163--182}},
  publisher    = {{Academic Press}},
  series       = {{Methods in Enzymology}},
  title        = {{[7] NMR studies of complex DNA structures : The holliday junction intermediate in genetic recombination}},
  url          = {{http://dx.doi.org/10.1016/S0076-6879(95)61009-X}},
  doi          = {{10.1016/S0076-6879(95)61009-X}},
  volume       = {{261}},
  year         = {{1995}},
}