A recent intermezzo at the Ribosome Club
(2017) In Philosophical Transactions of the Royal Society B: Biological Sciences 372(1716).- Abstract
Two sets of ribosome structures have recently led to two different interpretations of what limits the accuracy of codon translation by transfer RNAs. In this review, inspired by this intermezzo at the Ribosome Club, we briefly discuss accuracy amplification by energy driven proofreading and its implementation in genetic code translation. We further discuss general ways by which the monitoring bases of 16S rRNA may enhance the ultimate accuracy (d-values) and how the codon translation accuracy is reduced by the actions of Mg2+ ions and the presence of error inducing aminoglycoside antibiotics. We demonstrate that complete freezing-in of cognate-like tautomeric states of ribosome-bound nucleotide bases in transfer RNA or... (More)
Two sets of ribosome structures have recently led to two different interpretations of what limits the accuracy of codon translation by transfer RNAs. In this review, inspired by this intermezzo at the Ribosome Club, we briefly discuss accuracy amplification by energy driven proofreading and its implementation in genetic code translation. We further discuss general ways by which the monitoring bases of 16S rRNA may enhance the ultimate accuracy (d-values) and how the codon translation accuracy is reduced by the actions of Mg2+ ions and the presence of error inducing aminoglycoside antibiotics. We demonstrate that complete freezing-in of cognate-like tautomeric states of ribosome-bound nucleotide bases in transfer RNA or messenger RNA is not compatible with recent experiments on initial codon selection by transfer RNA in ternary complex with elongation factor Tu and GTP. From these considerations, we suggest that the sets of 30S subunit structures from the Ramakrishnan group and 70S structures from the Yusupov/Yusupova group may, after all, reflect two sides of the same coin and how the structurally based intermezzo at the Ribosome Club may be resolved simply by taking the dynamic aspects of ribosome function into account. This article is part of the themed issue ‘Perspectives on the ribosome’.
(Less)
- author
- Pavlov, Michael Y. ; Liljas, Anders LU and Ehrenberg, Måns
- organization
- publishing date
- 2017-03-19
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Initial transfer RNA selection, Proofreading, Ribosome, Tautomers, Translation accuracy
- in
- Philosophical Transactions of the Royal Society B: Biological Sciences
- volume
- 372
- issue
- 1716
- article number
- 20160185
- publisher
- Royal Society Publishing
- external identifiers
-
- scopus:85011697921
- pmid:28138071
- wos:000393403600008
- ISSN
- 0962-8436
- DOI
- 10.1098/rstb.2016.0185
- language
- English
- LU publication?
- yes
- id
- 6d4f7e9c-2b3b-458b-ad44-8736248c78a4
- date added to LUP
- 2017-02-20 08:53:20
- date last changed
- 2025-01-07 07:33:41
@article{6d4f7e9c-2b3b-458b-ad44-8736248c78a4,
abstract = {{<p>Two sets of ribosome structures have recently led to two different interpretations of what limits the accuracy of codon translation by transfer RNAs. In this review, inspired by this intermezzo at the Ribosome Club, we briefly discuss accuracy amplification by energy driven proofreading and its implementation in genetic code translation. We further discuss general ways by which the monitoring bases of 16S rRNA may enhance the ultimate accuracy (d-values) and how the codon translation accuracy is reduced by the actions of Mg<sup>2+</sup> ions and the presence of error inducing aminoglycoside antibiotics. We demonstrate that complete freezing-in of cognate-like tautomeric states of ribosome-bound nucleotide bases in transfer RNA or messenger RNA is not compatible with recent experiments on initial codon selection by transfer RNA in ternary complex with elongation factor Tu and GTP. From these considerations, we suggest that the sets of 30S subunit structures from the Ramakrishnan group and 70S structures from the Yusupov/Yusupova group may, after all, reflect two sides of the same coin and how the structurally based intermezzo at the Ribosome Club may be resolved simply by taking the dynamic aspects of ribosome function into account. This article is part of the themed issue ‘Perspectives on the ribosome’.</p>}},
author = {{Pavlov, Michael Y. and Liljas, Anders and Ehrenberg, Måns}},
issn = {{0962-8436}},
keywords = {{Initial transfer RNA selection; Proofreading; Ribosome; Tautomers; Translation accuracy}},
language = {{eng}},
month = {{03}},
number = {{1716}},
publisher = {{Royal Society Publishing}},
series = {{Philosophical Transactions of the Royal Society B: Biological Sciences}},
title = {{A recent intermezzo at the Ribosome Club}},
url = {{http://dx.doi.org/10.1098/rstb.2016.0185}},
doi = {{10.1098/rstb.2016.0185}},
volume = {{372}},
year = {{2017}},
}