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A recent intermezzo at the Ribosome Club

Pavlov, Michael Y.; Liljas, Anders LU and Ehrenberg, Måns (2017) In Royal Society of London. Philosophical Transactions B. Biological Sciences 372(1716).
Abstract

Two sets of ribosome structures have recently led to two different interpretations of what limits the accuracy of codon translation by transfer RNAs. In this review, inspired by this intermezzo at the Ribosome Club, we briefly discuss accuracy amplification by energy driven proofreading and its implementation in genetic code translation. We further discuss general ways by which the monitoring bases of 16S rRNA may enhance the ultimate accuracy (d-values) and how the codon translation accuracy is reduced by the actions of Mg2+ ions and the presence of error inducing aminoglycoside antibiotics. We demonstrate that complete freezing-in of cognate-like tautomeric states of ribosome-bound nucleotide bases in transfer RNA or... (More)

Two sets of ribosome structures have recently led to two different interpretations of what limits the accuracy of codon translation by transfer RNAs. In this review, inspired by this intermezzo at the Ribosome Club, we briefly discuss accuracy amplification by energy driven proofreading and its implementation in genetic code translation. We further discuss general ways by which the monitoring bases of 16S rRNA may enhance the ultimate accuracy (d-values) and how the codon translation accuracy is reduced by the actions of Mg2+ ions and the presence of error inducing aminoglycoside antibiotics. We demonstrate that complete freezing-in of cognate-like tautomeric states of ribosome-bound nucleotide bases in transfer RNA or messenger RNA is not compatible with recent experiments on initial codon selection by transfer RNA in ternary complex with elongation factor Tu and GTP. From these considerations, we suggest that the sets of 30S subunit structures from the Ramakrishnan group and 70S structures from the Yusupov/Yusupova group may, after all, reflect two sides of the same coin and how the structurally based intermezzo at the Ribosome Club may be resolved simply by taking the dynamic aspects of ribosome function into account. This article is part of the themed issue ‘Perspectives on the ribosome’.

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publication status
published
subject
keywords
Initial transfer RNA selection, Proofreading, Ribosome, Tautomers, Translation accuracy
in
Royal Society of London. Philosophical Transactions B. Biological Sciences
volume
372
issue
1716
publisher
Royal Society
external identifiers
  • scopus:85011697921
  • wos:000393403600008
ISSN
0962-8436
DOI
10.1098/rstb.2016.0185
language
English
LU publication?
yes
id
6d4f7e9c-2b3b-458b-ad44-8736248c78a4
date added to LUP
2017-02-20 08:53:20
date last changed
2018-05-06 04:31:03
@article{6d4f7e9c-2b3b-458b-ad44-8736248c78a4,
  abstract     = {<p>Two sets of ribosome structures have recently led to two different interpretations of what limits the accuracy of codon translation by transfer RNAs. In this review, inspired by this intermezzo at the Ribosome Club, we briefly discuss accuracy amplification by energy driven proofreading and its implementation in genetic code translation. We further discuss general ways by which the monitoring bases of 16S rRNA may enhance the ultimate accuracy (d-values) and how the codon translation accuracy is reduced by the actions of Mg<sup>2+</sup> ions and the presence of error inducing aminoglycoside antibiotics. We demonstrate that complete freezing-in of cognate-like tautomeric states of ribosome-bound nucleotide bases in transfer RNA or messenger RNA is not compatible with recent experiments on initial codon selection by transfer RNA in ternary complex with elongation factor Tu and GTP. From these considerations, we suggest that the sets of 30S subunit structures from the Ramakrishnan group and 70S structures from the Yusupov/Yusupova group may, after all, reflect two sides of the same coin and how the structurally based intermezzo at the Ribosome Club may be resolved simply by taking the dynamic aspects of ribosome function into account. This article is part of the themed issue ‘Perspectives on the ribosome’.</p>},
  articleno    = {20160185},
  author       = {Pavlov, Michael Y. and Liljas, Anders and Ehrenberg, Måns},
  issn         = {0962-8436},
  keyword      = {Initial transfer RNA selection,Proofreading,Ribosome,Tautomers,Translation accuracy},
  language     = {eng},
  month        = {03},
  number       = {1716},
  publisher    = {Royal Society},
  series       = {Royal Society of London. Philosophical Transactions B. Biological Sciences},
  title        = {A recent intermezzo at the Ribosome Club},
  url          = {http://dx.doi.org/10.1098/rstb.2016.0185},
  volume       = {372},
  year         = {2017},
}