X-ray and neutron μCT of biomedical samples: from image acquisition to quantification
(2021)- Abstract
- Even though the validity of x-ray computed tomography in the analysis of biomedical samples is nowadays undisputed, the more recent imaging techniques and more advanced instruments (such as synchrotrons) are still relatively unknown to many medical doctors that could benefit from them.
The doctoral work presented in this thesis joins a collective effort from the imaging community to demonstrate potential applications of advanced x-ray and neutron imaging methods to preclinical medical research, with the hope of contributing to reach a “critical mass” in the medical community and in the public opinion as well.
Two main lines of work are detailed, one focused on the ex vivo evaluation of corrosion processes of magnesium-based... (More) - Even though the validity of x-ray computed tomography in the analysis of biomedical samples is nowadays undisputed, the more recent imaging techniques and more advanced instruments (such as synchrotrons) are still relatively unknown to many medical doctors that could benefit from them.
The doctoral work presented in this thesis joins a collective effort from the imaging community to demonstrate potential applications of advanced x-ray and neutron imaging methods to preclinical medical research, with the hope of contributing to reach a “critical mass” in the medical community and in the public opinion as well.
Two main lines of work are detailed, one focused on the ex vivo evaluation of corrosion processes of magnesium-based biodegradable implants for osteosynthesis, the other dedicated to the assessment of neuropathy in human gastroenteric dysmotility. The aimed endpoint was to develop pipelines, from image acquisition all the way to data quantification, that could be used by other research groups with similar questions and may inspire future interdisciplinary collaborations between medicine, natural science and engineering.
In the first line of work, we have attempted to employ synchrotron-radiation micro-computed tomography (µCT) coupled with in situ loading tests to assess the mechanical properties of the bone-implant interface (Paper I). We have revealed the crucial importance of the radiation dose deposited on the sample, and that the mechanical loading geometry should be accurately determined in the planning steps of the experiment. Moving away from the mechanical testing, we have also explored a novel three-dimensional analysis of the corrosion by-products of biodegradable implants by combining x-ray µCT, neutron µCT and x-ray fluorescence mapping (Papers IV and V).
The second line of work has assessed the potential of x-ray phase-contrast µCT and nano-resolution holotomography as ways to perform virtual histology of unstained peripheral and autonomic neural tissue. In full-thickness biopsies of the myenteric nervous system, qualitative and potentially quantitative differences have been shown between controls and patients affected by gastrointestinal dysmotility (Paper II). In unstained skin biopsies, the methods have failed to visualise peripheral nerves, but we could identify structural changes in the connective tissue of some patients when compared to controls and other patients (Paper III). (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/6d58ced8-c1a6-418c-a2d3-8b4b98cc670d
- author
- Peruzzi, Niccoló LU
- supervisor
- opponent
-
- Prof. Dr. Müller, Bert, Biomaterials Science Center (BMC), Department of Biomedical Engineering, University of Basel, Basel, Switzerland
- organization
- publishing date
- 2021-11-22
- type
- Thesis
- publication status
- published
- subject
- keywords
- x-ray imaging, neutron imaging, μCT, synchrotron radiation, x-ray phase-contrast, nanoholotomography, x-ray elemental mapping, biomedical applications, virtual histology, biodegradable implants, Mg-alloys, enteric nervous system, peripheral neuropathy, gastroenteric dysmotility, Fysicumarkivet A:2021:Peruzzi
- pages
- 180 pages
- publisher
- Media-Tryck, Lund University, Sweden
- defense location
- Föreläsningssalen 2, Plan 1, Alwallhuset, Barngatan 2, Skånes universitetssjukhus, Lund. Join via zoom: https://www.msf.lu.se/evenemang/disputation-niccolo-peruzzi
- defense date
- 2021-12-16 09:00:00
- ISBN
- 978-91-8039-092-7
- 978-91-8039-091-0
- project
- X-ray and neutron μCT of biomedical samples: from image acquisition to quantification
- language
- English
- LU publication?
- yes
- id
- 6d58ced8-c1a6-418c-a2d3-8b4b98cc670d
- date added to LUP
- 2021-11-19 15:09:43
- date last changed
- 2022-08-18 10:38:57
@phdthesis{6d58ced8-c1a6-418c-a2d3-8b4b98cc670d,
abstract = {{Even though the validity of x-ray computed tomography in the analysis of biomedical samples is nowadays undisputed, the more recent imaging techniques and more advanced instruments (such as synchrotrons) are still relatively unknown to many medical doctors that could benefit from them.<br/>The doctoral work presented in this thesis joins a collective effort from the imaging community to demonstrate potential applications of advanced x-ray and neutron imaging methods to preclinical medical research, with the hope of contributing to reach a “critical mass” in the medical community and in the public opinion as well.<br/>Two main lines of work are detailed, one focused on the ex vivo evaluation of corrosion processes of magnesium-based biodegradable implants for osteosynthesis, the other dedicated to the assessment of neuropathy in human gastroenteric dysmotility. The aimed endpoint was to develop pipelines, from image acquisition all the way to data quantification, that could be used by other research groups with similar questions and may inspire future interdisciplinary collaborations between medicine, natural science and engineering.<br/>In the first line of work, we have attempted to employ synchrotron-radiation micro-computed tomography (µCT) coupled with in situ loading tests to assess the mechanical properties of the bone-implant interface (Paper I). We have revealed the crucial importance of the radiation dose deposited on the sample, and that the mechanical loading geometry should be accurately determined in the planning steps of the experiment. Moving away from the mechanical testing, we have also explored a novel three-dimensional analysis of the corrosion by-products of biodegradable implants by combining x-ray µCT, neutron µCT and x-ray fluorescence mapping (Papers IV and V). <br/>The second line of work has assessed the potential of x-ray phase-contrast µCT and nano-resolution holotomography as ways to perform virtual histology of unstained peripheral and autonomic neural tissue. In full-thickness biopsies of the myenteric nervous system, qualitative and potentially quantitative differences have been shown between controls and patients affected by gastrointestinal dysmotility (Paper II). In unstained skin biopsies, the methods have failed to visualise peripheral nerves, but we could identify structural changes in the connective tissue of some patients when compared to controls and other patients (Paper III).}},
author = {{Peruzzi, Niccoló}},
isbn = {{978-91-8039-092-7}},
keywords = {{x-ray imaging; neutron imaging; μCT; synchrotron radiation; x-ray phase-contrast; nanoholotomography; x-ray elemental mapping; biomedical applications; virtual histology; biodegradable implants; Mg-alloys; enteric nervous system; peripheral neuropathy; gastroenteric dysmotility; Fysicumarkivet A:2021:Peruzzi}},
language = {{eng}},
month = {{11}},
publisher = {{Media-Tryck, Lund University, Sweden}},
school = {{Lund University}},
title = {{X-ray and neutron μCT of biomedical samples: from image acquisition to quantification}},
url = {{https://lup.lub.lu.se/search/files/109917139/Niccolo_Peruzzi_summary_thesis_nopapers.pdf}},
year = {{2021}},
}